Evaluation of Extracts and Isolated Fraction from <i>Capparis spinosa</i> L. Buds as an Antioxidant Source

Germanò, Maria Paola; Pasquale, R. De; D’Angelo, Valeria; Catania, Stefania; Silvari, Virginia; Costa, Chiara

doi:10.1021/jf010678d

Cited by 142 publications

(104 citation statements)

References 17 publications

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“…DPPH free radical scavenging activity was determined according to Germano et al [18]. The extracts of stevia leaves, calli and roots were evaluated in terms of their radical scavenging ability using DPPH radical.…”

Section: Sample Preparation and Analytical Determinationsmentioning

confidence: 99%

Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert)

et al. 2016

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Background and purpose: Beside being rich with sweet glycosides, Stevia rebaudiana Bertoni is an interesting source of flavonoids and phenols. The current study aimed to assess the potential for increasing total phenols and flavonoids in S. rebaudiana tissue by varying concentrations of plant growth regulators (PGR) and pH levels of media. Materials and methods:The culture was established from seeds and propagated shoots were cultured on media of different pH levels (4.6, 5.8 and 7.4) and PGR. Total polyphenolics and free radical scavenging capability of leaf, callus and root extracts were determined. Shoot height, root length, shoot and root number were also recorded. Results: Shoot elongation and root development was stimulated by singly applied growth regulators though the values of both parameters were also satisfactory in PGR-free media. In the latter media, pH value of 4.6 was a main factor for increasing leaf metabolite levels. A most significant rise in phenols and flavonoids was evident in response to combination of BA either with GA3 or IAA compared to singly applied regulators indicating synergistic effects of PGR (especially of auxins and cytokinins). However, polyphenolics levels and their distribution between different tissues were also influenced by medium pH value. A positive correlation was found between antioxidant activity of S. rebaudiana extracts and phenols and flavonoids. Conclusions: The results show that PGR and medium pH value strongly affect accumulation of secondary metabolites and can lead to significant enhancement in productivity of bioactive polyphenolics in S. rebaudiana plant cultures. INTRODUCTION S tevia rebaudianaBertoni is a small perennial shrub of the Asteraceae family, native to South and Central America. S. rebaudiana occurs naturally on infertile, acid (pH 4 to 5) soils, but thrives with soil pH as high as 7.5 [1,2]. The plant is known worldwide principally for its high content of sweet diterpene glycosides, rebaudioside A and stevioside (the most prevalent glycoside) in leaf tissue and is being used as a low-calorie and non-toxic sweetener. Moreover, antimicrobial, antiviral, anticarcinogenic and antioxidant activity of S. rebaudiana leaf extracts was demonstrated by a number of studies. S. rebaudiana is also a good source of inulin-type carbohydrates, proteins, essential oils, minerals, vitamins as S. Radić et al. Influence of pH and plant growth regulators on Stevia rebaudiana 10Period biol, Vol 118, No 1, 2016.well as flavonoids and phenolic compounds [3]. Similar to other plant secondary metabolites, polyphenols have roles in protection against infections, attraction of pollinators and seed-dispersing animals, as allelopathic agents and are generally involved in plant defense against ultraviolet radiation. However, these bioactive compounds have attracted much scientific attention due to their beneficial effects on human health, notably in the prevention of various diseases associated with oxidative stress. The antioxidant effects of polyphenols are co...

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Section: Sample Preparation and Analytical Determinationsmentioning

confidence: 99%

Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert)

et al. 2016

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“…In addition, this observation is certainly associated with the low content of phenolic, isothyocyanate and nitrogen constituents in the three investigated oils (Germano et al 2002;Khoobchandani et al 2010;Ramandeep et al 2011). In addition, it is known that the synergistic or antagonistic effect of a compound present in minor percentage in a mixture has to be considered as well (Germano et al 2002). In the Brassicaceae family, the polyphenol compounds have been studied in relation to their content and antioxidant capacity.…”

Section: Radical Cation Abts +á Scavenging Activitymentioning

confidence: 88%

Chemical composition and biological activities ofEruca vesicariasubsp. longirostrisessential oils

Hichri

Mosbah

Majouli

et al. 2016

Pharmaceutical Biology

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Context To date, there are no reports to validate the Tunisian traditional and folklore claims of Eruca vesicaria (L) Cav. subsp. longirostris (Brassicaceae) for the treatment of disease. Objective Investigation of the chemical composition antimicrobial and antioxidant activity of essential oils from Eruca longirostris leaves, stems, roots and fruits. Materials and methods The essential oils of E. longirostris from leaves, stems, roots and fruits were obtained after 4 h of hydrodistillation. Chemical compositions were determined using a combination of GC/FID and GC/MS. The in vitro antimicrobial activity of the volatile constituents of E. longirostris was performed in sterile 96-well microplates against three Gram-positive, four Gram-negative bacteria and one strain as yeast. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration values were reported. Furthermore, the antioxidant activity was evaluated by DPPH and ABTS assays. Results The main compound for fruits, stems and roots was the erucin (96.6%, 85.3% and 83.7%, respectively), while b-elemene (35.7%), hexahydrofarnesylacetone (23.9%), (E)-b-damascone (15.4%), erucin (10.6%) and a-longipinene (9.6%) constituted the major compounds in the essential oil of the leaves. The experimental results showed that in all tests, essential oil of fruits showed the better antioxidant activity than the others. On the other hand, the oils of stems, fruits and roots showed significant antimicrobial activity with MIC values ranging from 0.125 to 0.31 mg/mL against Candida species, Gram-positive and Gram-negative bacteria, mainly Salmonella enterica. Conclusions The present results indicate that essential oils of E. longirostris can be used as a source of erucin. ARTICLE HISTORY

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“…The antioxidant potential was evaluated by spectrophotometric assay according to the method of Germano et al, 2002 in the visible region, using a solution of 0.004% DPPH in methanol, which was added to the solution of test samples. Stock solutions of extracts and standards were prepared to obtain a final concentration of 250 µg/ml (methanolic solution 20% Tween 80).…”

Section: Determination Of Antioxidant Activity With 1 1 Diphenyl 2-mentioning

confidence: 99%

Antiulcer Effects of Ethyl acetate Fraction of Carpolobia lutea Leaf

Nwidu¹

2012

J. App. Pharm. Sci.

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Carpolobia lutea leaves (CLL) (Polygalaceae) were earlier screened and the antiulcer ethnomedicinal claim established. This article seeks to quantitatively isolate, elucidate the active compounds from most active CLL fraction. Fractionation was by semi-preparative HPLC; the active fraction was subjected to radical scavenging assays (RSA) and quantification of the total phenolic content (TPC) were also executed. Results: Ethyl acetate fraction (EAF) was observed to be the most pharmacologically active antiulcer fraction when screened using acute ulcer models induced in rodents. The EAF demonstrated significant (p < 0.05-0.001) antiulcer activity in various in vivo induced ulceration models by reducing the ulcer index and increasing the preventive ratio. The EAF demonstrated > 70% in TPC and < 20 % in RSA. Cinnamic and coumaric acids derivatives were isolated from EAF. Cinnamic acids have been implicated and patented as antiulcer agent. Isolated compounds could in part mediate the observed pharmacological activities which lend credence to its ethnobotanical uses.

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Evaluation of Extracts and Isolated Fraction from Capparis spinosa L. Buds as an Antioxidant Source

Cited by 142 publications

References 17 publications

Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert)

Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert)

Chemical composition and biological activities ofEruca vesicariasubsp. longirostrisessential oils

Antiulcer Effects of Ethyl acetate Fraction of Carpolobia lutea Leaf

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