Evaluation of extraction methods for recovery of fatty acids from Botryococcus braunii LB 572 and Synechocystis sp. PCC 6803

Abstract

^Äëíê~Åí= Microalgae are a very diverse group of organisms that consist in both prokaryotic and eukaryotic forms. Some species of microalgae can be induced to overproduce particular fatty acids through simple manipulations of the physical and chemical properties of the culture medium. In this paper, the effect of different extraction techniques on the recovery of fatty acids from the freeze-dried biomass from two lipid-producing microalgal strains: _çíêóçÅçÅÅìë= Äê~ìåáá LB 572 (green algae) and póåÉÅÜçÅóëíáë sp. PCC 6803 (cyanobacteria) was examined. Five procedures were used: after conversion of the lipid material into the corresponding fatty acid methyl esters (FAMEs) îá~ suitable derivatization reactions (extraction-transesterification) and direct transesterification of biomass to produce FAMEs (without the initial extraction step) that used differential types of catalysts and processing conditions. This study has shown that procedure 3, a one step practical procedure for lipid extraction and áå=ëáíì methyl ester derivation could be used successfully for the determination of the fatty acid compositions of microalgae and cyanobacteria. © KSBB hÉóïçêÇëW=Ñ~ííó=~ÅáÇëI=íê~åëÉëíÉêáÑáÅ~íáçåI=ãáÅêç~äÖ~ÉI=Botryococcus braunii i_=RTOI=Synechocystis=ëéK=m``=SUMP=

Suitability of Soxhlet Extraction to Quantify Microalgal Fatty Acids as Determined by Comparison with In Situ Transesterification

“…Standard transesterification procedures [8] and recent investigations into ISTE of microalgal biomass [12,40] were used to develop lipid derivatization protocols. For two-step lipid derivatization (TE), a maximum of 20 mg of lipids extracted with the Soxtec apparatus were dried under nitrogen at room temperature to constant weight in pre-weighed Pyrex tubes (previously baked at 450°C overnight to minimize lipid contamination).…”

“…Other examples include spectrofluorometric determinations where lipid bodies are directly stained and quantified [6], supercritical CO 2 extraction [13] and Soxhlet extraction [30,17,22]. It is clear that whichever method is chosen, careful attention is required to optimize the extraction conditions-pre-processing is in some cases essential [27], as is reaction time and reagent concentration [12,40].…”

“…With these advantages in mind, a standardized ISTE protocol was developed in this laboratory, which was based on previously published standard transesterification procedures [8] as well as information from several recent investigations into ISTE of microalgal lipids [12,40].…”

Screening and evaluation of non-heterocystous filamentous cyanobacteria for lipid and commercially viable fatty acids

“…Microalgae accumulate large amounts of lipids as reserve material, with especially in conditions of stress and slow growth [9]. The fatty acid composition of marine microalgae has been studied more extensively [10]; [11]; [12]; [13] than the freshwater forms [14]; [15]; [16]. Thus there is need to screen fresh water cyanobacteria for lipids of commercial value.…”

Optimalization of Extraction Conditions for Increasing Microalgal Lipid Yield by Using Accelerated Solvent Extraction Method (ASE) Based on the Orthogonal Array Design

“…Traditionally, there could be several methods used in extraction of microalgal lipid to get information on lipid content of biological samples, for instance, the Soxhlet extraction method by using organic solvents for biological sample treatment and sample-heating treatment with some strong inorganic acids and so on. [71][72][73][74] .…”