Evaluation of extenders and cryopreservatives for cooling and cryopreservation of spermatozoa from the western spotted skunk (<i>Spilogale gracilis</i>)

Kaplan, Joyce B.; Mead, Rodney A.

doi:10.1002/zoo.1430110606

Cited by 5 publications

(2 citation statements)

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“…Equex STM (also called Orvus ES paste) has been used successfully in the pig [27,28] and with mixed results in a number of exotic animals [29,30]. When used in egg yolk-contain- ing diluents, Equex STM appears to enhance resistance of spermatozoa to cooling/freezing damage, possibly by solubilizing and making available to spermatozoa the protective egg yolk-derived lipids and lipoproteins [30,31].…”

Section: Discussionmentioning

confidence: 98%

Effect of Cryopreservation Methods and Precryopreservation Storage on Bottlenose Dolphin (Tursiops truncatus) Spermatozoa1

Robeck

O’Brien

2004

Biology of Reproduction

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Research was conducted to develop an effective method for cryopreserving bottlenose dolphin (Tursiops truncatus) semen processed immediately after collection or after 24-h liquid storage. In each of two experiments, four ejaculates were collected from three males. In experiment 1, three cryopreservation methods (CM1, CM2, and CM3), two straw sizes (0.25 and 0.5 ml), and three thawing rates (slow, medium, and fast) were evaluated. Evaluations were conducted at collection, prefreeze, and 0-, 3-, and 6-h postthaw. A sperm motility index (SMI; total motility [TM] x % progressive motility [PPM] x kinetic rating [KR, scale of 0-5]) was calculated and expressed as a percentage MI of the initial ejaculate. For all ejaculates, initial TM and PPM were greater than 85%, and KR was five. At 0-h postthaw, differences in SMI among cryopreservation methods and thaw rates were observed (P < 0.05), but no effect of straw size was observed. In experiment 2, ejaculates were divided into four aliquots for dilution (1:1) and storage at 4 degrees C with a skim milk- glucose or a N-tris(hydroxymethyl)methyl-2-aminoethane sulfonic acid (TES)-TRIS egg yolk solution and at 21 degrees C with a Hepes-Tyrode balanced salt solution (containing bovine albumin and HEPES) (TALP) medium or no dilution. After 24 h, samples were frozen and thawed (CM3, 0.5-ml straws, fast thawing rate) at 20 x 10(6) spermatozoa ml(-1) (low concentration) or at 100 x 10(6) spermatozoa ml(-1) (standard concentration). The SMI at 0-h postthaw was higher for samples stored at 4 degrees C than for samples stored at 21 degrees C (P < 0.001), and at 6-h postthaw, the SMI was higher for samples frozen at the standard concentration than for samples frozen at the low concentration (P < 0.05). For both experiments, acrosome integrity was similar across treatments. In summary, a semen cryopreservation protocol applied to fresh or liquid-stored semen maintained high levels of initial ejaculate sperm characteristics.

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Section: Discussionmentioning

confidence: 98%

Effect of Cryopreservation Methods and Precryopreservation Storage on Bottlenose Dolphin (Tursiops truncatus) Spermatozoa1

Robeck

O’Brien

2004

Biology of Reproduction

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“…Attempts to apply a semen cryopreservation protocol to diapausing mustelid species such as the western spotted skunk (Kaplan and Mead 1992) and stoat ( Mustela erminea ) have been undertaken (LaFalci and Molinia 2007), but no live kids have been reported so far.…”

Section: Artificial Insemination and Semen Cryobanking In Mustelidaementioning

confidence: 99%

Reproductive Technologies Relevant to the Genome Resource Bank in Carnivora

Amstislavsky

Lindeberg

Luvoni

2011

Reprod Domestic Animals

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Carnivora is one of the most species-rich order of Mammalia. Some species, e.g. domestic cats, dogs and ferrets, are among the most popular pets; others, such as minks and farmed foxes, have economic value for the fur breeding industry. Still others, such as tigers, bears and other top predators, have great impact on the health of natural ecosystems. Most if not all Carnivora species have great cultural and aesthetic importance for man. There are enormous differences between mammalian species in reproductive physiology, and it is not surprising that reproductive technologies can be used with high efficiency with some animal groups, e.g. most farm animals and laboratory rodents, but are very laborious when used with Carnivora species, which often possess unique reproductive traits. The efficiency of assisted reproductive technology (ART) applied to semi-domestic, non-domestic and especially to endangered species of Carnivora remains extremely low in most cases, and often the first positive result reported is the only instance when ART has been successful with that species. Although there are approximately 270 species in the Carnivora order, to the best of our knowledge, successful published attempts to apply ART have been reported for only four families: Mustelidae, Felidae, Canidae and Ursidae. The main achievements in ART, embryo technology in particular, for these families of Carnivora, together with challenges and problems, are reviewed in the relevant sections.

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