Evaluation of Expression of 4 Mirnas in Cytological Samples as an Additional Method of Cervical Cancer Diagnosis

Архангельская, П. А.; Samsonov, Roman; Shtam, Tatiana; Князева, М. С.; Иванов, М. Ф.; Титов, С. Е.; Колесников, Н. Н.; Бахидзе, Е. В.; Berlev, Igor; Михетько, А. А.; Воробьев, С. Л.; Malek, Anastasia

doi:10.17650/1994-4098-2017-13-3-63-72

Cited by 3 publications

(2 citation statements)

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“…Potentially marker miRNAs were selected on the basis of the results of earlier published investigations and analysis of the scientific literature. Thus, the over-regulations of miR-145 and miR-1246 are associated with cervical dysplasia development, as reported previously [18,19]. The miR-1290 is a molecule involved in the development of squamous epithelial malignancies of various localizations, including oral and laryngeal squamous cell carcinoma [39].…”

Section: Novaprep-mir-cervix Performancesupporting

confidence: 67%

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NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

Kniazeva¹,

Zabegina²,

Shalaev³

et al. 2023

IJMS

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Cervical cancer is one of the most common gynecological malignancies and it is preventable through the yearly diagnosis and management of pre-cancerous cervical disease. The profile of miRNA expression in cervical epithelium cells is altered with cervical dysplasia development and further progression. The NOVAprep-miR-CERVIX is a new approach for the assessment of cervical dysplasia through the analysis of six marker miRNAs. This study aims to evaluate theperformance and diagnostic potency of the new method. Cytological smears from 226 women (NILM, n.114; HSIL, n.112) were included in the study. A VPH test was performed with RealBest DNAHPV HR screen Kit, six marker miRNAs (miR-21, -29b, -145, -451a, -1246, -1290) were assayed using NOVAprep-miR-CERVIX kit. Obtained data were analyzed using the Delta Ct method and random forest machine learning algorithm. The results of the quantitative analysis of six microRNAs were expressed as a miR-CERVIX parameter, which ranged from 0 to 1, where “0” corresponded to the healthy cervical epithelium, while “1” corresponded to high-grade squamous intraepithelial dysplasia. The average value of miR-CERVIX differed in groups of NILM and HSIL samples (0.34 vs. 0.72; p < 0.000005). An estimation of miR-CERVIX allowed for the differentiation between healthy and pre-cancerous samples with sensitivity of 0.79 and specificity of 0.79, as well as to confirm HSIL with specificity of 0.98. Interestingly, the HSIL group included HPV(+) and HPV(−) samples, which were statistically significantly different in terms of miR-CERVIX value. Analysis of CC-associated miRNAs in material of cervical smear might serve as an additional method for the evaluation of cervical dysplasia severity.

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Section: Novaprep-mir-cervix Performancesupporting

confidence: 67%

“…In our previous studies, we identified CC-associated miRNAs [17][18][19]. These results were combined with original published study analyses to select the potencial miRNA markers of cervical carcinogenesis (Table 1).…”

Section: Introductionmentioning

confidence: 99%

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

Kniazeva¹,

Zabegina²,

Shalaev³

et al. 2023

IJMS

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Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia

Князева

Присяжная

Zabegina

et al. 2021

Opuholi ženskoj reproduktivnoj sistemy

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Background. Currently, there are no reliable markers for the prognosis of the low-grade squamous intraepithelial lesion (LSIL) of the cervical epithelium. Scientific literature provides with inconsistent recommendations regarding the management of the young patients with a cytological diagnosis of LSIL. The progression of cervical dysplasia and the development of cervical cancer are associated with characteristic alterations of the microRNA expression profile.Objective: to assess the prognostic value of microRNA in LSIL. Materials and methods. Samples (cytological smears) obtained from patients diagnosed with LSIL (n = 36), but with a different course of the disease over the next 6–12 months. Analysis of miRNA expression was carried out by the method of “two-tailed” reverse transcription and subsequent PCR.Results. The expression level of miR-126, miR-21, miR-1246, miR-182 was statistically significantly different in the compared groups, but the predictive value of the analysis of individual molecules was low (AUC <0.65). Calculation of the concentration ratios of the “reciprocal” pairs of microRNAs made it possible to obtain a more effective prognostic marker. ROC analysis of such ratios (miR-126/miR-182, miR-21/miR-182, miR-1246/miR-182) yielded AUC values: 0.82–0.89, sensitivity: 0.71–0.92; specificity: 0.86.Conclusions. Analysis of a panel of microRNA marker molecules in the material of the cervical epithelium and calculation of the concentration ratios of “reciprocal” pairs is a promising method for prognosis of LSIL course.

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Diagnostic Value of Exosomal Mirnas for Colorectal Cancer

et al. 2018

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OBJECTIVE. Assessment of diagnostic significance of exosomal microRNAs analysis in colorectal cancer (CRC). MATERIALS AND METHODS. Plasma samples from 100 patients with colon tumors and 20 healthy donors. Exosomes were isolated by differential ultracentrif ugation the analysis was performed by dynamic light scattering (DLS), cryo-EM, flow cytometry. Quantitative analysis of exosomal microRNAs was performed by RT-PCR. To evaluate the results obtained, the Kraskel-Wallis statistical test and ROC analysis were used. RESULTS. It is shown that CRC causes characteristic changes in the concentration of a number of exosomal microRNAs. Analysis of «reciprocal miRNAs pairs» was proposed as algorithmfor personalized diagnostic of CRC. The optimal parameters of diagnostic values were obtained for miRNA pair«miR-223 / miR-181a» (sensitivity =0.93, specificity =0.88). CONCLUSION. The analysis of exosomal microRNAs presents a promising method for early diagnostics / screening of colorectal cancer.

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Evaluation of Expression of 4 Mirnas in Cytological Samples as an Additional Method of Cervical Cancer Diagnosis

Cited by 3 publications

References 42 publications

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia

Diagnostic Value of Exosomal Mirnas for Colorectal Cancer

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