Evaluation of existing control measures in reducing health and safety risks of engineered nanomaterials

Oksel, Ceyda; Subramanian, Vrishali; Semenzin, Elena; Yun, Cai; Hristozov, Danail; Wang, Xue Z.; Hunt, Neil; Costa, Anna Luisa; Fransman, Wouter; Marcomini, Antonio; Wilkins, Terry

doi:10.1039/c6en00122j

Cited by 13 publications

(8 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They can be tailor-made for specific medical and technological applications, and some of them have already found widespread use in consumer products. , Their concomitant release into the environment is unavoidable, leading to unintended human exposure to NPs. Serious risks to human health may result upon NP uptake through the lung, gut, or skin. − Important examples of intended NP exposure in biomedical applications (mainly through intravenous injection) are contrast agents, for example, iron oxide NPs in magnetic resonance imaging, or drug carriers, such as lipid NPs in drug delivery. , …”

Section: Introductionmentioning

confidence: 99%

In Situ Characterization of Protein Adsorption onto Nanoparticles by Fluorescence Correlation Spectroscopy

2017

View full text Add to dashboard Cite

Nanotechnology holds great promise for applications in many fields including biology and medicine. Unfortunately, the processes occurring at the interface between nanomaterials and living systems are exceedingly complex and not yet well understood, which has significantly hampered the realization of many nanobiotechnology applications. Whenever nanoparticles (NPs) are incorporated by a living organism, a protein adsorption layer, also known as the "protein corona", forms on the NP surface. Accordingly, living organisms interact with protein-coated rather than bare NPs, and their biological responses depend on the nature of the protein corona. In recent years, a wide variety of biophysical techniques have been employed to elucidate mechanistic aspects of NP-protein interactions. In most studies, NPs are immersed in protein or biofluid (e.g., blood serum) solutions and then separated from the liquid for analysis. Because this approach may modify the composition and structure of the protein corona, our group has pioneered the use of fluorescence correlation spectroscopy (FCS) as an in situ technique, capable of examining NP-protein interactions while the NPs are suspended in biological fluids. FCS allows us to measure, with subnanometer precision and as a function of protein concentration, the increase in hydrodynamic radius of the NPs due to protein adsorption. This Account aims at reviewing recent progress in the exploration of NP-protein interactions by using FCS. In vitro FCS studies of the adsorption of important serum proteins onto water-solubilized luminescent NPs always showed a stepwise increase of the NP radius upon protein binding in the form of a binding isotherm, regardless of the type of NP and its specific surface functionalization. This observation indicates formation of a protein monolayer on the NP. Structure-based calculations of protein surface potentials revealed that positively charged patches on the proteins interact electrostatically with negatively charged NP surfaces, and the observed protein layer thickness always matched the known molecular dimensions of the proteins binding in certain orientations. Temperature and NP surface functionalization have also been identified as important parameters controlling protein corona formation. Notably, while the corona formed from a single type of serum protein was reversible, protein adsorption from complex biological media such as blood serum was entirely irreversible. These quantitative in vitro studies are of great relevance to the bio-nano community and especially to researchers developing engineered nanomaterials for biological and biomedical applications. Future efforts will be directed toward elucidating kinetic aspects of protein corona formation and the detailed structure of the adsorbed proteins at the molecular level. To better appreciate the biological responses triggered by NP exposure, more efforts will be devoted to the exploration of the biomolecular corona as it forms on NPs in contact with living cells, tissues, and even entire model...

show abstract

Section: Introductionmentioning

confidence: 99%

In Situ Characterization of Protein Adsorption onto Nanoparticles by Fluorescence Correlation Spectroscopy

2017

View full text Add to dashboard Cite

show abstract

“…In addition, in this step, suitable risk management measures (RMMs) for the relevant scenarios are selected from those showing efficacy in controlling nanomaterials (e.g. gloves, filtering masks and suitable ventilation as reported in Oksel et al 2016) and are communicated to product developers and restorers for a safe manufacturing and application of the selected products (stage 3).…”

Section: Methodsmentioning

confidence: 99%

Guiding the development of sustainable nano-enabled products for the conservation of works of art: proposal for a framework implementing the Safe by Design concept

Semenzin

Giubilato

Badetti

et al. 2019

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Nanotechnology provides innovative and promising solutions for the conservation of cultural heritage, but the development and application of new nano-enabled products pose concerns regarding their human health and environmental risks. To address these issues, we propose a sustainability framework implementing the Safe by Design concept to support product developers in the early steps of product development, with the aim to provide safer nano-formulations for conservation, while retaining their functionality. In addition, this framework can support the assessment of sustainability of new products and their comparison to their conventional chemical counterparts if any. The goal is to promote the selection and use of safer and more sustainable nano-based products in different conservation contexts. The application of the proposed framework is illustrated through a hypothetical case which provides a realistic example of the methodological steps to be followed, tailored and iterated along the decision-making process.

show abstract

“…37 With the aim of controlling nanomaterial exposure at the workplace, various existing exposure control measures have recently been evaluated. 38 the use of suitable protective clothing such as a Tyvek suit with a hood, nitrile gloves, and eye/face protection such as goggles.…”

Section: T H Imentioning

confidence: 99%

“…37 With the aim of controlling nanomaterial exposure at the workplace, various existing exposure control measures have recently been evaluated. 38 This review includes a number of technical reports and guidelines published by (inter)national organisations and standardization bodies including NIOSH, EPA, OECD, and ISO that are aimed at controlling nanomaterial exposure at the workplace.…”

mentioning

confidence: 99%

Considerations for Safe Innovation: The Case of Graphene

et al. 2017

View full text Add to dashboard Cite

The terms "Safe innovation" and "Safe(r)-by-design" are currently popular in the field of nanotechnology. These terms are used to describe approaches that advocate the consideration of safety aspects already at an early stage of the innovation process of (nano)materials and nanoenabled products. Here, we investigate the possibilities of considering safety aspects during various stages of the innovation process of graphene, outlining what information is already available for assessing potential hazard, exposure, and risks. In addition, we recommend further steps to be taken by various stakeholders to promote the safe production and safe use of graphene.

show abstract

Evaluation of existing control measures in reducing health and safety risks of engineered nanomaterials

Abstract: While the risk management of engineered nanomaterials (ENMS) receives significant attention, there is still a limited understanding of how to select optimal risk management measures for reducing the risks of ENMs.

Cited by 13 publications

References 45 publications

In Situ Characterization of Protein Adsorption onto Nanoparticles by Fluorescence Correlation Spectroscopy

In Situ Characterization of Protein Adsorption onto Nanoparticles by Fluorescence Correlation Spectroscopy

Guiding the development of sustainable nano-enabled products for the conservation of works of art: proposal for a framework implementing the Safe by Design concept

Considerations for Safe Innovation: The Case of Graphene

Contact Info

Product

Resources

About