Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing

L'Huillier, Arnaud G; Hamid-Allie, Anne; Kristjanson, Erik; Papageorgiou, Louis; Hung, Sam; Wong, Chun Fai; Stein, Derek; Olsha, Romy; Goneau, Lee W.; Dimitrova, Kristina; Drebot, Mike; Safronetz, David; Gubbay, Jonathan B.

doi:10.1128/jcm.00442-17

Cited by 92 publications

(83 citation statements)

References 16 publications

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“…The assessment of ZIKV in urine or whole blood would have been a valuable addition to the study protocol and should be considered in future studies 28. The lack of detected IgM responses may be due to the limited sensitivity of the serological method used for IgM detection (Euroimmun ZIKV ELISA) 29, 30. Furthermore, IgM responses can be attenuated in infected individuals with flavivirus infections in the past31, 32, 33 and ZIKV serology is further complicated by extensive cross‐reactivity with other endemic flaviviruses 21, 34.…”

Section: Discussionmentioning

confidence: 99%

Zika virus and Guillain–Barré syndrome in Bangladesh

GeurtsvanKessel

Islam

et al. 2018

Ann Clin Transl Neurol

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ObjectivePrevious studies have associated Guillain–Barré syndrome (GBS) with Zika virus (ZIKV) outbreaks in South America and Oceania. In Asia, ZIKV is known to circulate widely, but the association with Guillain–Barré syndrome is unclear. We investigated whether endemic ZIKV infection is associated with the development of GBS.MethodsA prospective study was conducted from 2011 to 2015 in Bangladesh. A total of 418 patients and 418 healthy family controls were included in the study. Patients were diagnosed with GBS prior to inclusion according to established criteria. Detailed information on the epidemiology, clinical presentation, electrophysiology, diagnosis, disease severity, and clinical course were obtained during a follow‐up of 1 year using a predefined protocol.Results ZIKV‐neutralizing antibodies were detected in our study from 2013 onwards. The prevalence of ZIKV‐neutralizing antibodies was not significantly higher in patients with GBS compared to healthy controls (OR 2.23, P = 0.14, 95% CI 0.77–6.53). Serological evidence for prior ZIKV infection in patients with GBS was associated with more frequent cranial, sensory, and autonomic nerve involvement compared to GBS patients with Campylobacter jejuni, the predominant preceding infection in GBS worldwide. Nerve‐conduction studies revealed that ZIKV antibodies were associated with a demyelinating subtype of GBS, while C. jejuni infections were related to an axonal subtype.InterpretationNo significant association was found between ZIKV infection and GBS in Bangladesh, but GBS following ZIKV infection was characterized by a distinct clinical and electrophysiological subtype compared to C. jejuni infection. These findings indicate that ZIKV may precede a specific GBS subtype but the risk is low.

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Section: Discussionmentioning

confidence: 99%

Zika virus and Guillain–Barré syndrome in Bangladesh

GeurtsvanKessel

Islam

et al. 2018

Ann Clin Transl Neurol

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“…IgM and IgG antibodies specific for ZIKV nonstructural protein 1 (NS1) in serum, saliva, and urine were detected using a commercial Anti‐Zika virus IgM and IgG ELISA kit which used ZIKV NS1 recombinant protein (Euroimmun, Lübeck, Germany) as coating antigen. The assays were performed according to the manufacturer's instructions . The optical density (OD) values were read at 450 and 630 nm.…”

Section: Methodsmentioning

confidence: 99%

Kinetics of antigen‐specific IgM/IgG/IgA antibody responses during Zika virus natural infection in two patients

Zhao

Hong

Wang

et al. 2018

Journal of Medical Virology

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Understanding of kinetics of antibody responses is crucial for developing rapid serological tests and studying the mechanisms of Zika virus (ZIKV) infection. Most of the serological diagnostic assays previously published are based on either IgM or IgG titer, little is known on the level of IgA antibody in saliva and urine. In this study, we investigated the kinetics of IgM/IgG/IgA antibody responses in serum, saliva, and urine obtained from two ZIKV infected individuals from as early as the second day of onset of symptoms to as long as 2 years postinfection. Other than detecting robust early IgM response, long lasting IgG response, we discovered strong early IgA response specific for ZIKV in saliva in both patients. This unique observation provides a novel strategy and scientific basis for the development of noninvasive rapid tests for ZIKV infection.

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“…20 Our laboratory used the RealStar Zika Virus RT-PCR Kit by Altona that has a sensitivity and specificity of 91% and 97%, respectively. The ZIKV serology used in our study was EUROIMMUN Anti-ZIKV enzyme-linked immunosorbent assay (ELISA) IGM and IGG which is commercially available through Thermo Fisher Scientific, Inc., and it was validated in our laboratory to be performed manually, and it has a specificity of 93% and a sensitivity of 40%.…”

Section: Rates Of Positive Zikv Serology and Pcrmentioning

confidence: 99%

“…The ZIKV serology used in our study was EUROIMMUN Anti-ZIKV enzyme-linked immunosorbent assay (ELISA) IGM and IGG which is commercially available through Thermo Fisher Scientific, Inc., and it was validated in our laboratory to be performed manually, and it has a specificity of 93% and a sensitivity of 40%. 20 Our laboratory used the RealStar Zika Virus RT-PCR Kit by Altona that has a sensitivity and specificity of 91% and 97%, respectively. 21 The extraction method was either by NucliSENS, easyMag, or manual extraction using the Qiagen RNA Blood Mini Kit.…”

Section: Rates Of Positive Zikv Serology and Pcrmentioning

confidence: 99%

Screening for Zika virus in deceased organ donors in Florida

Simkins

Anjan

Morillas‐Rodriguez

et al. 2018

American Journal of Transplantation

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Zika virus (ZIKV) cases have been detected across the United States (US) and locally acquired cases have been reported in Florida. Currently, there are no ZIKV screening guidelines and no data on the incidence among organ donors in the US. This retrospective study was conducted at Jackson Memorial-Miami Transplant Institute. Positive ZIKV tests in local deceased organ donors were investigated from 6/2016 to 1/2017. We evaluated demographics and risk factors for ZIKV infection among organ donors and transplant outcomes among recipients of donors with positive ZIKV testing. One hundred forty-two donors were analyzed. Ten percent had traveled to ZIKV-endemic countries and 19% had outdoor occupations. Only 3% had positive ZIKV IGG. None had a positive ZIKV IGM or PCR. ZIKV-positive donors were more likely to have traveled to ZIKV-endemic countries (50% vs. 9%, P = .05). The kidneys from a ZIKV-positive donor were transplanted in our hospital with no 6-month rejection, graft failure, or death in the recipients. Our study demonstrated a low prevalence of ZIKV among deceased donors in our community. Despite local ZIKV transmission, ZIKV was more common in donors who traveled to ZIKV-endemic countries. This cohort demonstrated excellent outcomes in recipients of ZIKV IGG-positive donors. However, larger studies are needed.

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Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing

Cited by 92 publications

References 16 publications

Zika virus and Guillain–Barré syndrome in Bangladesh

Zika virus and Guillain–Barré syndrome in Bangladesh

Kinetics of antigen‐specific IgM/IgG/IgA antibody responses during Zika virus natural infection in two patients

Screening for Zika virus in deceased organ donors in Florida

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