Evaluation of estrogenic effects of polychlorinated biphenyls and organochlorinated pesticides using immature rat uterotrophic assay

Uslu, Ünal; Sandal, Süleyman; Cumbul, Alev; Yıldız, Sedat; Aydın, Mehmet; Yılmaz, Bayram

doi:10.1177/0960327112472999

Cited by 29 publications

(7 citation statements)

References 44 publications

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“…In addition, EPA guideline recommends analysis of phytoestrogen content in the diet in the case of high uterine control weight relative to historic controls or an inadequate response to the reference estrogen, which did not occur in the present study. Finally, the relation mean uterus weight (mg)/mean body weight (g) in the control group of this work was 0.64, whereas in studies conducted with immature Wistar rats a relation of 0.67 to 0.82 was present, 16 –19 supporting that the rats from our laboratory do not seem to be under exogenous estrogenic influence.…”

Section: Discussionsupporting

confidence: 64%

Evaluation of Escitalopram, Sertraline, and Methylphenidate in the Immature Rat Uterotrophic Assay

et al. 2013

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Psychotropics are among the most prescribed medications. There are indications in the literature that fluoxetine (FLX; selective serotonin reuptake inhibitor [SSRI] antidepressant) and methylphenidate (MPH) could have a hormonal mode of action. This study was designed to evaluate the immature rat uterotrophic assay Substitute by (1) if sertraline (SER) and escitalopram (ESC) 2 other SSRI antidepressants would share the estrogenicity described for FLX and (2) MPH for estrogenicity and antiestrogenicity. The 18-day-old Wistar rats with were divided into olive oil, estradiol (0.3 mg/kg), estradiol + tamoxifen (10 mg/kg), SER (5, 15, or 45 mg/kg), ESC (2, 6, or 18 mg/kg), MPH (2.5 or 5 mg/kg), and estradiol + MPH groups. As expected, estradiol increased the weight of uterus, and this effect was counterbalanced by concomitant treatment with tamoxifen. The SER, ESC, and MPH had no effect on the uterus weight. The results suggest that ESC and SER do not share the estrogenicity described for FLX and that MPH does not disrupt estrogenic signaling.

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Section: Discussionsupporting

confidence: 64%

Evaluation of Escitalopram, Sertraline, and Methylphenidate in the Immature Rat Uterotrophic Assay

et al. 2013

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“…However, the CPF group had the lowest FSH concentration when compared to other groups. This observation agreed with the previous work that pesticides, including CPF lowered the FSH level (Zidan, 2009;Mandal and Das, 2011;Uslu et al, 2013;Shittu et al, 2013). FSH, a member of pituitary glycoprotein, plays a dual role in spermatogenesis by directly affecting the sertoli cells to stimulate and initiate germ cell number and indirectly, enhances androgen production by the Leydig cells (O'Shaughnessy et al, 2010).…”

Section: Discussionsupporting

confidence: 92%

AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

Shittu

Aisha

Ambali

et al. 2014

American Journal of Pharmacology and Toxicology

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Studies have shown that Chlorpyrifos (CPF), an Organophosphate (OP) insecticide alters both sex and thyroid hormones. Apart from inhibiting Acetylcholinesterase (AChE) activity, CPF has been shown to cause oxidative stress. The antioxidant potentials of many flavonoid-containing plants are increasingly being exploited in the therapy of many infectious and non-infectious diseases. Hibiscus Sabdariffa (HS) is one of the most widely used nutraceuticals that has been used traditionally to combat various illnesses due to its high flavonoid contents. The present study was therefore aimed at evaluating the ameliorative potentials of HS on subchronic chlorpyrifos-evoked alterations in sex and thyroid hormones in male Wistar rats. Forty-two (42) young adult male Wistar rats were divided at random into six groups containing seven (7) rats per group. Group I was administered distilled water (2 mL kg −1 ) only while group II received soya oil (2 mL kg −1 ), Group III was dosed with only aqueous extract of HS (500 mL kg −1 ~ 1/10 th of the LD 50 ), while group IV was given CPF (10.6 mL kg −1 ~ 1/8 th of the LD 50 ). Group V was pretreated with low dose of HS (250 mg kg −1 ~ 1/20 th of the LD 50 ) and then administered reconstituted CPF (10.6 mg kg −1 ), 30 min later. Group VI was pretreated with high dose of the HS (500 mg kg −1 ) and then administered CPF (10.6 mg kg −1 ), 30 min later. The regimens were administered orally by gavage once daily for a period of 11 weeks. At the end of the treatment period, sera obtained from the blood samples were analyzed for the levels of Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), testosterone, thyroid hormones (T 3 , T 4 and TSH) and AChE. Thyroid and pituitary glands of each rat were also evaluated for Malondialdehyde (MDA) concentration. Results showed a non-significant (p>0.05) decrease in the concentrations of FSH, LH and testosterone in the CPF group relative to the other groups. There was significant decrease (p<0.05) in the concentrations of T 3, T 4 and an increase in TSH in the CPF group relative to the other groups. There were significant increases (p<0.05) in MDA concentrations in the thyroid and pituitary glands in the CPF group compared to the other groups. Pretreatment with aqueous extract of HS demonstrated a dose-dependent amelioration of CPF-induced alterations in the levels of testosterone, LH, FSH, AChE, T 3 , T 4 and TSH in the serum and that of pituitary and thyroid glands MDA. This may be partly due to its high level of polyphenolic compounds that confer its antioxidant and possibly AChE restoration AJPTactivities. It is therefore concluded that pretreatment of individuals who are occupationally exposed to CPF and probably other OPs with the extract of HS may result in protection from the insecticideinduced adverse reproductive health outcomes.

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“…Association between EDC exposures and diabetes has also been demonstrated in experimental studies. Reduced insulin levels were observed in adult mice exposed to DDT during the perinatal window of development [57]. A similar study in which mice were exposed to BPA in utero resulted in the impairment of insulin secretion and glucose tolerance [58].…”

Section: Diabetesmentioning

confidence: 89%

Endocrine-Disrupting Chemicals and Infectious Diseases: From Endocrine Disruption to Immunosuppression

Adegoke

Rahman

Park

et al. 2021

IJMS

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Endocrine-disrupting chemicals (EDCs) are hormonally active compounds in the environment that interfere with the body’s endocrine system and consequently produce adverse health effects. Despite persistent public health concerns, EDCs remain important components of common consumer products, thus representing ubiquitous contaminants to humans. While scientific evidence confirmed their contribution to the severity of Influenza A virus (H1N1) in the animal model, their roles in susceptibility and clinical outcome of the coronavirus disease (COVID-19) cannot be underestimated. Since its emergence in late 2019, clinical reports on COVID-19 have confirmed that severe disease and death occur in persons aged ≥65 years and those with underlying comorbidities. Major comorbidities of COVID-19 include diabetes, obesity, cardiovascular disease, hypertension, cancer, and kidney and liver diseases. Meanwhile, long-term exposure to EDCs contributes significantly to the onset and progression of these comorbid diseases. Besides, EDCs play vital roles in the disruption of the body’s immune system. Here, we review the recent literature on the roles of EDCs in comorbidities contributing to COVID-19 mortality, impacts of EDCs on the immune system, and recent articles linking EDCs to COVID-19 risks. We also recommend methodologies that could be adopted to comprehensively study the role of EDCs in COVID-19 risk.

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Evaluation of estrogenic effects of polychlorinated biphenyls and organochlorinated pesticides using immature rat uterotrophic assay

Cited by 29 publications

References 44 publications

Evaluation of Escitalopram, Sertraline, and Methylphenidate in the Immature Rat Uterotrophic Assay

Evaluation of Escitalopram, Sertraline, and Methylphenidate in the Immature Rat Uterotrophic Assay

AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

Endocrine-Disrupting Chemicals and Infectious Diseases: From Endocrine Disruption to Immunosuppression

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