Evaluation of estrogen in endometriosis patients: Regulation of GATA‐3 in endometrial cells and effects on Th2 cytokines

Chen, Peng; Wang, Danbo; Liang, Yanming

doi:10.1111/jog.12957

Cited by 31 publications

(23 citation statements)

References 46 publications

(81 reference statements)

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“…Endometriosis is an inflammatory disease involving a possible shift towards the Th2-mediated immune response [13,14]. As a Th2 cell-specific transcription factor, GATA-3 has been found to play important roles in cell proliferation and differentiation of the mammary glands [15], nephric duct [16] and nervous system [17].…”

Section: Discussionmentioning

confidence: 99%

GATA-3 Stimulates Proliferation of Endometriotic Cells

2019

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Objectives: This study aimed to investigate whether there was a correlation between the expression of GATA binding protein 3 (GATA-3) and the proliferation of endometrium cells from women with endometriosis. Material and methods: Endometrial tissue was collected from 20 women with endometriosis (experimental group) and 10 women without endometriosis (control group). The primary endometrial cells were then cultured and identified. Each group of cells were further divided into four groups. The control group was divided into control-no treatment (C-N), control-negative control (C-NC, transfected with a scrambled siRNA negative control), control-lentiviral transfection control (C-LC) and control-GATA-3 lentiviral transfection (C-G3); the experimental group was divided into experiment-no treatment (E-N), experiment-negative control (E-NC), experiment-lentiviral transfection control (E-LC) and experiment-siGATA-3 lentiviral transfection (E-siG3). RT-PCR and western blot were used respectively to detect the endometrium cells' GATA-3 mRNA and protein expression. Further, Cell Counting Kit-8 was used to measure the endometrium cells' proliferation. Results: The relative expression of GATA-3 mRNA and protein in the E-N group was significantly higher than in the D-N group and E-siG3 group (P < 0.05). The relative cell proliferation rate in the C-G3 group at 48 h, 72 h and 96 h was significantly higher than in the C-N, C-NC and C-LC groups (P < 0.05), while in the experimental group, the proliferation rate of the E-siG3 group was significantly lower than in the E-N, E-NC and E-LC groups (P < 0.05). Conclusions: This study suggests the role of GATA-3 in promoting proliferation of endometrial cells; therefore, GATA-3 may play an essential role in the development of endometriosis.

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Section: Discussionmentioning

confidence: 99%

GATA-3 Stimulates Proliferation of Endometriotic Cells

2019

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“…Endometriosis202122235474 is an oestrogen-dependent disorder defined by the ectopic growth of endometrium-like tissue (reviewed in ref. 75), which represents the leading cause of women infertility767778.…”

Section: Discussionmentioning

confidence: 99%

An evolutionary conserved interaction between the Gcm transcription factor and the SF1 nuclear receptor in the female reproductive system

Cattenoz¹,

Delaporte²,

Bazzi³

et al. 2016

Sci Rep

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NR5A1 is essential for the development and for the function of steroid producing glands of the reproductive system. Moreover, its misregulation is associated with endometriosis, which is the first cause of infertility in women. Hr39, the Drosophila ortholog of NR5A1, is expressed and required in the secretory cells of the spermatheca, the female exocrine gland that ensures fertility by secreting substances that attract and capacitate the spermatozoids. We here identify a direct regulator of Hr39 in the spermatheca: the Gcm transcription factor. Furthermore, lack of Gcm prevents the production of the secretory cells and leads to female sterility in Drosophila. Hr39 regulation by Gcm seems conserved in mammals and involves the modification of the DNA methylation profile of mNr5a1. This study identifies a new molecular pathway in female reproductive system development and suggests a role for hGCM in the progression of reproductive tract diseases in humans.

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“…Eutopic endometrial tissues from patients with endometriosis have higher mRNA levels of GATA-binding protein 3 (GATA3) compared to normal endometrial tissue (Chen et al 2012). Expression of GATA3 is regulated by estrogen, and their synergistic action regulates Th2 cytokine (e.g., IL6, IL8 and IL10) expression in eutopic endometrial cells (Chen et al 2016). Therefore, GATA3 integrates estrogen signaling to induce Th2 cytokine expression in endometriotic lesions, thereby promoting endometriosis progression.…”

Section: Role and Determinants Of Th2 Cytokine Production During Endomentioning

confidence: 99%