Evaluation of Epidermal Growth Factor-Incorporating Skin Care Product in Culture Experiment Using Human Fibroblasts

Yamamoto, Akiko; Ohno, Haruka; Yoshimitsu, Kengo

doi:10.4236/ojrm.2016.52004

Cited by 7 publications

(6 citation statements)

References 23 publications

(21 reference statements)

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“…PGA is known to form a soft, smooth, self-elastic and moisturizing film on skin and stimulate natural moisturizing factors, and its 3D molecule structure network allows for high water retention with humectant activity [10,21,[23][24][25][26][27]. In another study, an enhanced human-derived fibroblasts proliferation has been observed after being treated with a skin care sponge, containing poly-(γ-glutamic acid) [28]. PGA esters have been observed to modify collagen gels on human skin fibroblast cell lines HFF-1 and 3T3 [29,30].…”

Section: Discussionmentioning

confidence: 99%

Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications

et al. 2020

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Several exopolymers with different chemical composition and correspondingly variety in their physico-chemical properties from halophilic microorganisms have still been described, however, with a low production yield. Chromohalobacter canadensis 28 isolated from Pomorie saltern synthesized an unusual exopolymer (EP) containing 72% γ-polyglutamic acid (PGA), an essential cosmeceutical additive. Current work suggests a novel approach for effective EP synthesis by C. canadensis 28 using continuous cultures. Highest production was observed at low dilution rates reaching a level of 2.1 mg/mL at D = 0.035, similar to those in batch cultures (2.34 mg/mL), however avoiding all disadvantages of discontinuous fermentation processes. At steady state, the total quantities of the synthesized EP after 48 h cultivation for the given equipment volume in D = 0.035 h−1 and D = 0.075 h−1 were 8.67 and 12 g, correspondingly, while it was 2.9 g for batch culture. Process parameters did not change after a ten-day run at D = 0.35 h−1. A degree of purity of EP fraction received from continuous cultures was significantly increased up to 93–96%. A lack of cytotoxicity and high cell viability were observed for human dermal fibroblast cells after 24 h incubation with crude EP from C. canadensis 28 and purified PGA fraction that could suggest its high potential for cosmetic applications.

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Section: Discussionmentioning

confidence: 99%

Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications

et al. 2020

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“…This skin care product is water-soluble, because of no intermolecular cross-linking among HA and Col. This skin care product (5 cm × 8 cm) contains EGF at a concentration of 1.0 µg/cm 2 [45].…”

Section: Skin Care Product Using Growth Factor and Biomaterialsmentioning

confidence: 99%

“…The result demonstrated that fibroblasts in collagen gel sheet released 3.7-times more VEGF (Figure 14) and 25-times more HGF in +EGF group compared with −EGF group (Figure 15). EGF-incorporating skin care product has a potential to stimulate effectively fibroblast to synthesize an increased amount of VEGF and HGF [45].…”

Section: Fundamental Study Using Egf-incorporating Skin Care Productmentioning

confidence: 99%

Advanced Treatment of Burns and Skin Ulcers Using Tissue-Engineered Products

Kuroyanagi¹,

Yoshimitsu²

2018

Dermatol Case Rep

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Three types of tissue-engineered products have been introduced in this review. The first product is an allogeneic cultured dermal substitute. This product is manufactured by incorporating allogeneic fibroblasts into a spongy sheet of hyaluronic acid and collagen. Allogeneic fibroblasts are rejected gradually in immune system. However, they are able to release some growth factors that are essential for wound healing. Hyaluronic acid and collagen also have a potential to promote wound healing. The clinical study demonstrated that this cultured dermal substitute is useful for the treatment of burns and skin ulcers. The second product is a functional wound dressing. This product is manufactured by freeze-drying an aqueous solution of hyaluronic acid, collagen, and epidermal growth factor. Epidermal growth factor has a potential to promote wound healing by enhancing both keratinocyte and fibroblast proliferation, and also by stimulating fibroblasts to synthesize vascular endothelial growth factor and hepatocyte growth factor that are essential for angiogenesis. The clinical study demonstrated that this wound dressing is useful for the treatment of burns and skin ulcers. The third product is a skin care product. This product is manufactured by freeze-drying an aqueous solution of hyaluronic acid, collagen, epidermal growth factor, and other ingredients for skin care. The experiment using a culture system demonstrated that this skin care product stimulates fibroblast to synthesize an increased amount of vascular endothelial growth factor and hepatocyte growth factor. This skin care product is aimed to use for post-treatment of chemical peeling and laser therapy used in aesthetic dermatology.

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“…Base on the results related to the skin care product described above, another skin care product was developed [56]. This commercial available skin care product is composed of HA and Col containing EGF and VC, supplemented with GC, PGA, and Arg as cosmetic ingredients (EGF-skin care product).…”

Section: Skin Care Product Composed Of Hyaluronic Acid and Collagen Smentioning

confidence: 99%

Tissue-Engineered Products for Skin Regenerative Medicine

Yoshimitsu¹

2016

OJRM

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In a general wound healing process, foreign bodies and tissue detritus have to be broken down and then a new tissue is produced. However, the new tissue formation sometimes fails to proceed under the impaired conditions such as burn injury and intractable skin ulcer. A major obstruction to wound healing is infection. Another obstruction to wound healing is deficiency of growth factors. The endogenous levels of growth factors are reduced in some chronic wounds. To improve these wound conditions, researchers have been trying to create several types of artificial skins. The tissue-engineered products include three prime constituents, i.e., cells, growth factors, and materials. In this review, the practical design of tissue-engineered products for skin regenerative medicine is introduced. The first design makes it possible to release silver sulfadiazine (AgSD) from a wound dressing. The second design makes it possible to release Epidermal Growth Factor (EGF) from a wound dressing or a skin care product composed of hyaluronic acid spongy sheet containing bioactive ingredients. The third design makes it possible to release several types of growth factors from allogeneic fibroblasts within cultured dermal substitute. This tissue-engineered product is prepared by seeding allogeneic fibroblasts into a collagen and hyaluronic acid spongy sheet. Although allogeneic cells are rejected gradually in immune system, they are able to release some types of growth factors, thereby regenerating a damaged tissue. The clinical study demonstrates that these tissue-engineered products are promising for the treatment of burn injury and intractable skin ulcer.

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Evaluation of Epidermal Growth Factor-Incorporating Skin Care Product in Culture Experiment Using Human Fibroblasts

Cited by 7 publications

References 23 publications

Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications

Improved Exopolymer Production by Chromohalobacter canadensis Cultures for Its Potential Cosmeceutical Applications

Advanced Treatment of Burns and Skin Ulcers Using Tissue-Engineered Products

Tissue-Engineered Products for Skin Regenerative Medicine

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