Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis

Yang, J.; Kennedy, M T

doi:10.1128/jcm.10.6.778-785.1979

Cited by 33 publications

(9 citation statements)

References 14 publications

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“…These immunoassays detect 90 to 100% of amoebic liver abscess, 80 to 100% of symptomatic intestinal amebiasis, and 10 to 50% of asymptomatic cyst passers (9,15,17). Recently, enzyme immunoassays (enzyme-linked immunosorbent assay; ELISA) using microtiter plates or plastic tubes as the antigen carrier have been reported for the detection of human antibodies to Entamoeba histolytica (1,2,22,24,26). Our laboratory has developed a simple standardized semiquantitative ELISA procedure in which the reported results are related to the presence or absence of E. histolytica antibodies at levels sufficient to be detectable by gel diffusion methods, a level which appears to be clinically sig-nificant.…”

mentioning

confidence: 99%

Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica

Lin¹,

Halbert²,

Chiu³

et al. 1981

J Clin Microbiol

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A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-linked immunosorbent assay is rapid (1 h 15 min, total incubation time), and the reported values are referenced to a positive control so that they correlate with levels of antibody sufficient to be detected by the gel diffusion methods. The enzyme-linked immunosorbent assay is highly reproducible, specific, and sensitive; it can be used qualitatively or quantitatively.

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mentioning

confidence: 99%

Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica

Lin¹,

Halbert²,

Chiu³

et al. 1981

J Clin Microbiol

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“…Previously reported studies using the ELISA in the serological diagnosis of invasive amoebiasis have used poorly defined soluble antigens (2,24,26). More recently, highly antigenic membrane fractions of 27 to 220 kilodaltons have been identified by using either monoclonal or polyclonal antibody probes (11,15,16,19,25).…”

Section: Discussionmentioning

confidence: 99%

Detection of Entamoeba histolytica immunoglobulins G and M to plasma membrane antigen by enzyme-linked immunosorbent assay

et al. 1990

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Sixty-one serum specimens from 22 patients with clinically diagnosed amoebic liver abscess (ALA), 10 hospitalized patients with a variety of diseases other than amoebiasis, 12 normal healthy controls, and 17 subjects from an amoebiasis-endemic area were assayed by enzyme-linked immunosorbent assay (ELISA). The plasma membrane fraction of axenic cultures of Entamoeba histolytica HK9 separated from other subcellular fractions by differential centrifugation was used as the antigen to detect specific immunoglobulin G (IgG) and IgM antibodies. Using a single serum dilution of 1/100 and optical densities at 492 nm of 0.200 and 0.250 as the cutoff values for the IgM and IgG ELISAs, their respective sensitivities in 22 ALA patients were 91% (20 of 22) and 95% (21 of 22). In 22 patients (10 hospitalized and 12 normal healthy controls), the specificities of the IgM and IgG ELISAs were 95% (21 of 22) and 91% (20 of 22), respectively. Ali five asymptomatic carriers of pathogenic E. histolytica were seropositive by the IgG ELISA and the amoebic gel diffusion test (AGDT). The AGDT was positive for three of six culture-negative controls, while the IgG ELISA was positive for all six. For six asymptomatic carriers of nonpathogenic zymodemes, the AGDT was positive for two, and the IgG ELISA was positive for three. There was an excellent correlation (r = 0.96) between the IgG ELISA and the AGDT. Only one of six culture-negative controls, none of the asymptomatic carriers of pathogenic E. histolytica, and one of six carriers of nonpathogenic E. histolytica were seropositive by the IgM ELISA, thus highlighting the specificity of the IgM ELISA in the diagnosis of ALA. It is believed that the use of plasma membrane fractions has improved the diagnostic potential of the IgM ELISA.

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“…In ELISA, the classic technique involves passive adsorption of antigen in alkaline diluents such as carbonate, bicarbonate, carbonate-bicarbonate, glycine, or borate buffers (9,13,38,65). Alternatively, antigen may be bound at physiological pH values by using diluents such as PBS (12,53) or water (55,72). Fixative and coupling agents for improving antigen retention on support media, such as formaldehyde (12,25), glutaraldehyde (1,55,64), acetone (57), and poly-L-lysine (14,37) have also been used.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

Barlough¹,

Jacobson²,

Downing³

et al. 1983

J Clin Microbiol

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A computer-assisted, kinetics-based enzyme-linked immunosorbent assay was adapted for the detection of coronavirus antibodies in feline serum. An alkaline antigen diluent (carbonate-bicarbonate buffer, pH 9.6) used in initial experiments produced diffuse, nonspecific color reactions in both viral and control antigen cuvettes which were correlated, paradoxically, with coronavirus antibody levels in test sera. These interfering reactions were minimized by use of lower-pH antigen diluents such as water and phosphate-buffered saline. Background kinetics-based enzyme-linked immunosorbent assay reactivity directed against a noncoronaviral component of antigen tissue culture fluids could then detected in numerous sera, particularly in samples with lower titers. Much of this reactivity was shown to be associated with bovine gamma globulins in cell culture fluid. It was not serum lot or species specific, since a variety of bovine serum lots as well as individual lots of serum from other mammalian and avian species reacted. Reactivity was markedly reduced when cells for antigen preparation were grown in gamma globulin-free bovine serum. Generation of corrected slope values from the kinetics-based enzyme-linked immunosorbent assay made it possible to correct for residual background reactivity in individual test sera and thus eliminate a potentially major source of false-positive reactions. Collectively, these studies indicated that the control of nonspecific reactivity in feline coronavirus serology is absolutely essential to obtain useful estimates of specific antibody responses.

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Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis

Cited by 33 publications

References 14 publications

Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica

Simple standardized enzyme-linked immunosorbent assay for human antibodies to Entamoeba histolytica

Detection of Entamoeba histolytica immunoglobulins G and M to plasma membrane antigen by enzyme-linked immunosorbent assay

Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats

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