Evaluation of Enterococcus hirae LAB3 as potential probiont against Vibrio harveyi in Artemia nauplii and Asian seabass larvae (Lates calcarifer) cultures

Masduki, Fathiah; Zakaria, T.; Min, Chong Chou; Karim, Murni

doi:10.22438/jeb/41/5(si)/ms_06

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…Supplementation with autochthonous probiotic bacterium has been reported for several fish species (Sun et al, 2013;Ghorbani Vaghei et al, 2019;Masduki et al, 2020;Sousa et al, 2020). Studies have demonstrated the effects of supplementation with probiotic monostrains on improving zootechnical performance and animal immunocompetence (Masduki et al, 2020;Sousa et al, 2020;Suphoronski et al, 2021;Hossain et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…The administration of probiotic via live foods such as artemia and rotifers, in the feeding of fish and crustacean larvae, has gained increasing interest in aquaculture (Lobo et al, 2018;Ghoname et al, 2020;Samat et al, 2021;Oliveira et al, 2022). Previous studies showed higher growth and survival rates, as well as higher protease, amylase, and lipase activities in Macrobrachium rosenbergii post-larvae supplemented with Enterococcus durans enriched in Artemia franciscana (Bhaheerathan et al, 2020), greater resistance against the pathogen Vibrio harveyi in larvae Lates calcarifer supplemented via Artemia with Enterococcus hirae (Masduki et al, 2020) and higher n-3 HUFA levels in Solea senegalensis larvae (Lobo et al, 2018). Overall, the introduction of probiotics through enrichment of live diets showed positive results, with higher growth and survival rates (Avella et al, 2010;Bhaheerathan et al, 2020), in addition to improving the immune responses of fish (Sun et al, 2013;Ghoname et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus

Barros,

Dias,

Abe

et al. 2023

BIP

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The research investigated the effect of dietary supplementation with Artemia sp. enriched with the autochthonous probiotic Enterococcus faecium on growth performance, microbiota modulation, intestinal morphology, and resistance to pathogenic bacteria of Megaleporinus macrocephalus larvae. The study evaluated four treatments (C: without probiotics; T1: 1 × 104 ; T2:1× 106 ; and T3: 1 × 108 CFU·mL-1) in quadruplicates. The larvae (n = 160; weight = 5.3 ± 2.3mg and length = 3.73±0.4mm) were distributed in 16 L containers at a density of 10 larvae·L-1 for 20 days. The productive performance, survival, gut microbiology, and histology were measured. The larvae were also submitted to acute challenge against the pathogenic bacterium Aeromonas hydrophila. The results showed that supplementation with 1 × 108 CFU·mL-1 promotes greater gain in length (13.78 ± 0.40 cm) and total weight (0.08 ± 0.002 g), higher counts of lactic acid bacteria and lower total heterotrophic in the intestines (7.11±0.30; 0.12 ± 0.09 log CFU·g-1, respectively) and larger villi (0.26 ± 0.03μm). Diets containing probiotics influenced the animals’ resistance to acute infection, with a lower accumulated mortality in T3 (33% ± 11.54%) and a higher one in C+ (93% ± 11.54%). Thus, probiotic supplementation with the autochthonous bacterium E. faecium (1×108CFU·mL-1) provides zootechnical improvement, villus increase and greater resistance to infections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus

Barros,

Dias,

Abe

et al. 2023

BIP

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“…MSP1 and 2 strains come from a variety of genera, including Bacillus , Lysinibacillus , and Enterococcus . Although the use of probiotics from the genus Enterococcus is uncommon in aquaculture, earlier research has examined this species and demonstrated its potential as a probiotic in aquaculture [ 32 , 33 , 34 ]. For instance, the use of E. hirae enhanced the juvenile hybrid catfish in terms of growth and survival when challenged with A. hydrophila [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

Puvanasundram

Chong

Sabri

et al. 2022

Biology

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Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. Bacillus amyloliquefaciens L11, Enterococcus hirae LAB3, and Lysinibacillus fusiformis SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to Aeromonas hydrophila and Streptococcus agalactiae than a single probiotic. MSP 2 candidates were Lysinibacillus fusiformis strains SPS11, A1, and Lysinibacillus sphaericus strain NAS32 because the inhibition zone produced by MSP 2 against Vibrio harveyi and Vibrio parahaemolyticus was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (p < 0.05) A. hydrophila count from 9.89 ± 0.1 CFU mL−1 to 2.14 ± 0.2 CFU mL−1. The biofilm formation of both MSPs were significantly higher (p < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species.

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“…This method is based on Masduki et al [ 60 ] with minor modifications. Ten M. micrura were placed in a 50 mL Falcon tube containing 30 mL sterilized freshwater pond water.…”

Section: Methodsmentioning

confidence: 99%

The Efficacy of Moina micrura Enriched with Probiotic Bacillus pocheonensis in Enhancing Survival and Disease Resistance of Red Hybrid Tilapia (Oreochromis spp.) Larvae

et al. 2021

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The administration of probiotics via live feeds, such as Artemia and rotifers, has gained significant attention. Moreover, indiscriminate use of antibiotics in conventional aquaculture practices in order to prevent or control disease outbreaks has resulted in the occurrence of residues and antimicrobial resistance. Thus, the application of eco-friendly feed additives, such as probiotics, as a safer alternative has received increasing attention in recent years. However, only minimal information on the administration of probiotics via freshwater cladoceran Moina micrura is available despite being commonly used for larval and post-larval feeding of freshwater crustaceans and fish. Thus, this study aimed to evaluate the application of Bacillus pocheonensis strain S2 administered via M. micrura to red hybrid tilapia (Oreochromis spp.) larvae. Bacillus pocheonensis that has been previously isolated from Spirulina sp. was subjected to preliminary in vitro evaluation of antagonistic properties. The agar well-diffusion assay revealed that this probiont could inhibit the growth of Streptococcus agalactiae and Aeromonas hydrophila. The size of inhibition zones ranged from 8.8 ± 0.2 to 18.2 ± 0.4 mm. Moina micrura was later used as a biological model in preliminary in vivo bacterial challenge assays to evaluate the efficacy of B. pocheonensis in protecting the host from diseases. Moina micrura was pre-enriched with B. pocheonensis at 104 and 106 CFU mL-1 before S. agalactiae and A. hydrophila were introduced into the culture. The study revealed that B. pocheonensis at 104 CFU mL-1 was able to significantly enhance the survival of M. micrura after being challenged with both pathogens (63 ± 3%) in comparison to the control group. The relative percentage survival (RPS) of M. micrura was highest (p < 0.05) when treated with B. pocheonensis at both concentrations 104 and 106 CFU mL-1 (38.33) after being challenged against S. agalactiae. To assess the efficacy of B. pocheonensis in protecting red hybrid tilapia against streptococcosis, the larvae were fed with either unenriched (control) Moina or probiont-enriched Moina daily for 10 days. A significantly (p < 0.05) higher survival rate (77 ± 3%) was observed in larvae fed with probiont-enriched M. micrura compared to other treatments, and the RPS was recorded at 62.90. In addition, the S. agalactiae load was suppressed in larvae fed probiont-enriched M. micrura (6.84 ± 0.39 CFU mL-1) in comparison to the control group (7.78 ± 0.09 CFU mL-1), indicating that the probiont might have contributed to the improvement of tilapia health and survival. This study illustrated that M. micrura was suitable to be used as a vector for probiotics in freshwater fish larvae as an alternative to hazardous antibiotics for disease control.

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Evaluation of Enterococcus hirae LAB3 as potential probiont against Vibrio harveyi in Artemia nauplii and Asian seabass larvae (Lates calcarifer) cultures

Cited by 5 publications

References 23 publications

Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus

Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

The Efficacy of Moina micrura Enriched with Probiotic Bacillus pocheonensis in Enhancing Survival and Disease Resistance of Red Hybrid Tilapia (Oreochromis spp.) Larvae

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