Evaluation of enrichment with antioxidants from olive oil mill wastes in hydrophilic model system

Romeo, Rosa; Bruno, Alessandra De; Imeneo, Valeria; Piscopo, Amalia; Poiana, Marco

doi:10.1111/jfpp.14211

Cited by 29 publications

(27 citation statements)

References 34 publications

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“…The antioxidant activity of WE was measured by mean of DPPH and ABTS •+ assays. Results obtained with the ABTS assay were higher as mmol TE 100 mL −1 than those obtained by DPPH, according to our previous study [ 32 ]. As reported in the literature, antioxidant activity is due to the synergism between the various phenolic compounds and the assay responses are affected by the functional group’s reactivity and characteristics, reaction time and complexity of the reaction kinetics [ 41 , 42 ].…”

Section: Resultssupporting

confidence: 55%

“…In addition, the antioxidant activity of MBoil samples was also analysed by ORAC assay according to our previous study [ 32 ]. The ORAC assay was carried out on VICTOR X2 2030 Multilabel Plate Readers (PerkinElmer, Boston, Massachusetts, USA) in 96-well black microplate (PerkinElmer, Boston, Massachusetts, USA) using a fluorescence filter with an excitation wavelength of 485 nm and emission wavelength of 520 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Identification and determination of the principal bioactive phenolic compounds of WE and MBoil were performed by UHPLC in accordance with [ 32 ]. The UHPLC system consisted of an UHPLC PLATINblue (Knauer, Berlin, Germany) equipped with a binary pump system using a Knauer blue orchid column C18 (1.8 μm, 100 × 2 mm) coupled with a PDA--1 (Photo Diode Array Detector) PLATINblue (Knauer, Berlin, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Impact of Stability of Enriched Oil with Phenolic Extract from Olive Mill Wastewaters

Romeo

Bruno

Imeneo

et al. 2020

Foods

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The disposal of olive mill wastewaters is a considerable subject for the development of a sustainable olive oil industry considering their high content of pollutants. Nevertheless, the selective extraction of phenolic compounds from olive mill wastewaters represents a promising approach to obtain phenolics suitable for food enrichment. This work aimed to evaluate the efficiency of phenolic extract addition (50 mg L−1), used as natural antioxidant, in sunflower oil against oxidative deterioration; to this aim, XAD-7-HP resin was tested in the recovery of phenolic compounds from olive mill wastewaters. Ultra-high performance liquid chromatography was used to evaluate the single phenols contained in the extract; the most consistent amount was detected for hydroxytyrosol (834 mg 100 mL−1). The change in the oxidation state of fortified sunflower oil was studied by measuring physicochemical (refractive index, peroxide value and oxidative resistance to degradation) and antioxidant parameters (DPPH, ABTS and ORAC assays) during 90 days of storage. Results showed an enhancement of oxidative stability of 50% in the fortified oil compared to control.

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Section: Resultssupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Impact of Stability of Enriched Oil with Phenolic Extract from Olive Mill Wastewaters

Romeo

Bruno

Imeneo

et al. 2020

Foods

Self Cite

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“…Identification and quantification of chemical compounds were performed by using ultra-high-performance liquid chromatography (UHPLC) following the method previously described by Romeo et al [ 38 ]. This system consisted of an UHPLC PLATIN blue (Knauer, Germany) equipped with a binary pump system using a Knauer column C18 (100 × 2 mm, 1.8 μm) coupled with a Photo Diode Array Detector (PDA)-1 PLATIN blue (Knauer, Germany).…”

Section: Methodsmentioning

confidence: 99%

Olive Mill Wastewater Polyphenol-Enriched Fractions by Integrated Membrane Process: A Promising Source of Antioxidant, Hypolipidemic and Hypoglycaemic Compounds

et al. 2020

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The valorisation of food wastes is a challenging opportunity for the green, sustainable, and competitive development of industry. The recovery of phenols contributes to the sustainability of olive waste sector, reducing its environmental impact and promoting the development of innovative formulations of interest for pharmaceutical, nutraceutical, and cosmeceutical applications. In this work, olive mill wastewater was treated through a combination of microfiltration (MF), nanofiltration (NF), and reverse osmosis (RO) in a sequential design to produce polyphenol-enriched fractions that have been investigated for their chemical profile using ultra-high-performance liquid chromatography (UHPLC), and their potential antioxidant, hypolipidemic, and hypoglycaemic activities. RO retentate exhibited the highest content of hydroxytyrosol, tyrosol, oleuropein, verbascoside, vanillic acid, and luteolin. In particular, a content of hydroxytyrosol of 1522.2 mg/L, about five times higher than the MF feed, was found. RO retentate was the most active extract in all in vitro tests. Interestingly, this fraction showed a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radicals scavenging activity with an IC50 value of 6.9 μg/mL and a potential inhibition of lipid peroxidation evaluated by the β-carotene bleaching test with IC50 values of 25.1 μg/mL after 30 min of incubation. Moreover, RO retentate inhibited α-amylase and α-glucosidase with IC50 values of 65.3 and 66.2 μg/mL, respectively.

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“…Vitamin C degradation, microbial spoilage, and off flavours production cause quality loss and reduction of orange juice shelf life (Kaddumukasa et al, 2017). The use of synthetic and natural antioxidants in the formulation of functional foods is of great and actual interest to prevent or delay the autoxidation process (Faustino et al, 2019;Romeo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effects of phenolic enrichment on vitamin C and antioxidant activity of commercial orange juice

Romeo

Bruno

Piscopo

et al. 2020

Braz. J. Food Technol.

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The autoxidation process of vitamin C in orange juice is the most important cause of quality loss during its storage. We evaluated the enrichment with different concentrations of a phenolic extract in a commercial orange juice for some qualitative parameters such as the content of vitamin C, phenolic compounds, and antioxidant activity. The lowest concentration of phenols produced the most stable enriched juice. In particular, we observed an inverse correlation between phenolic concentration and vitamin C retention in enriched juices. DPPH assay results confirmed this trend, correlated more to the vitamin antioxidant effect. TEAC results instead, were similar for the different juice samples, probably influenced by the phenolic content. The application of this study is the production for the industry of new functional drinks such as juices enriched with phenolic ingredients that show increased stability concerning those without addiction.

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Evaluation of enrichment with antioxidants from olive oil mill wastes in hydrophilic model system

Cited by 29 publications

References 34 publications

Impact of Stability of Enriched Oil with Phenolic Extract from Olive Mill Wastewaters

Impact of Stability of Enriched Oil with Phenolic Extract from Olive Mill Wastewaters

Olive Mill Wastewater Polyphenol-Enriched Fractions by Integrated Membrane Process: A Promising Source of Antioxidant, Hypolipidemic and Hypoglycaemic Compounds

Effects of phenolic enrichment on vitamin C and antioxidant activity of commercial orange juice

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