Evaluation of Endometrial Urocortin Secretion for Prediction of Pregnancy after Intrauterine Insemination

Florio, Pasquale; Bruni, L; Falco, Carmen De; Filardi, Gilda; Torricelli, Michela; Reis, Fernando M.; Galleri, Letizia; Voltolini, Chiara; Bocchi, Caterina; Leo, Vincenzo De

doi:10.1373/clinchem.2007.094987

Cited by 13 publications

(11 citation statements)

References 29 publications

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“…Uterine fluid includes a variety of cytokines, which provide a suitable microenvironment for embryo implantation ( 29 ). Previous studies confirmed that intrauterine fluid aspiration during the window of implantation did not affect the success rate of pregnancy in women seeking pregnancy, such as patients who accepted intrauterine insemination or IVF-ET ( 30 , 31 ). In addition, intrauterine fluid aspiration also had no effect on pregnancy in infertile patients with endometriosis, or patients with idiopathic infertility or luteal phase deficiency ( 32 ).…”

Section: Discussionmentioning

confidence: 80%

Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome

Wang

et al. 2021

Front. Endocrinol.

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ObjectiveNumerous studies have reported on ovulation and pregnancy rates in patients with polycystic ovary syndrome (PCOS). However, relevant data on endometrial receptivity are limited. This study was conducted to compare endometrial receptivity during implantation windows among letrozole (LE), clomiphene citrate (CC), and natural cycle, and to assess the predictive value for pregnancy of observed indicators.MethodsThis randomized controlled trial study enrolled 270 patients with PCOS. Patients were given LE (n=90) at a dose of 2.5mg/day or CC (n=90) at a dose of 50 mg/day on cycle days 5–9 for ovulation induction. Patients in the natural cycle group (n=90) did not receive any drug for ovulation induction. Endometrial ultrasonic parameters, integrin αvβ3, and vascular endothelial growth factor (VEGF) concentrations in uterine secretion were detected during the implantation window. The endometrial receptivity, ovulation rate, pregnancy rates, and predictive value of observed indicators for pregnancy were analyzed.ResultsThe successful ovulation rate did not differ between the LE group and CC group (P>0.05). Endometrial ultrasonic parameters [endometrial thickness (ET), endometrial volume (EV), vascularization index (VI), flow index (FI), vascularization flow index (VFI)], integrin αvβ3, and VEGF concentrations in uterine fluid were significantly higher in the LE group compared with the CC group and natural cycle group (P<0.05). The clinical pregnancy and ongoing pregnancy rates of the LE group were significantly higher than in the CC group (P<0.05). Endometrial ultrasonic parameters (VI, FI, and VFI), integrin αvβ3, and VEGF concentrations in uterine fluid of all pregnancy groups were significantly higher compared with the no pregnancy group (P<0.05), and the above parameters in ongoing pregnancy were significantly higher than in biochemical pregnancy (P<0.05). The endometrial FI during the implantation window had the highest predictive value for pregnancy (AUC=0.889). The integrin αvβ3 in uterine fluid had better predictive value (AUC=0.876) than VEGF.ConclusionsEndometrial receptivity during the implantation window of LE is superior to CC in PCOS women, which may be related to higher clinical pregnancy and ongoing pregnancy rates. Endometrial FI examined by 3-D power Doppler, and integrin αvβ3 in uterine secretion during the implantation window, could be preferable non-invasive predictor markers for pregnancy.Clinical Trial Registrationwww.chictr.org.cn, ChiCTR1900023423.

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Section: Discussionmentioning

confidence: 80%

Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome

Wang

et al. 2021

Front. Endocrinol.

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“…The latter data may be due to the fact that tubal decidualization is not generally observed in ectopic pregnancy [29,30]. Ucn1 is highly expressed in the secretory endometrium [13] and in EVT cells [14], promotes decidualization in-vitro [13], and is only produced in large amounts by an endometrium receptive to a presenting embryo [16].…”

Section: Discussionmentioning

confidence: 99%

“…We also showed that Ucn1 mRNA expression is lower in decidualized endometrium from women with an ectopic compared to a normal intrauterine pregnancy. A recent study showed that Ucn1 levels are directly related to the degree of decidualization of the endometrium [13] and a direct correlation between Ucn1 concentrations in endometrial flushings and successful implantation has been reported [16]. Additionally, locally produced CRH, which shares 45% homology with Ucn1 [7] and acts with lower affinity than Ucn1 through the same receptors [8,9], has been reported to promote implantation and maintenance of early pregnancy, primarily by inducing apoptosis in activated T cells at the maternal-fetal interface, allowing controlled trophoblast invasion and implantation into maternal decidua [19].…”

Section: Discussionmentioning

confidence: 99%

“…Ucn1 is highly expressed by human extravillous trophoblast cells (EVT) (the invasive part of the placenta) [14] and it has been reported to have a potent vasodilator action on the placental bed [15]. Furthermore, higher Ucn1 levels have been observed in endometrial flushings from women who achieved pregnancy after intrauterine insemination compared to those who did not, suggesting that Ucn1 may only be secreted in appreciable amounts by a receptive endometrium [16]. Both CRH-R1 and CRH-R2 [17,18] are also expressed in the human endometrium throughout the menstrual cycle but only CRH-R1 is expressed on EVT cells [19].…”

Section: Introductionmentioning

confidence: 99%

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Attenuated Tubal and Endometrial Urocortin 1 and Corticotropin-Releasing Hormone Receptor Expression in Ectopic Pregnancy

et al. 2011

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Fallopian tube (FT) and endometrial urocortin 1 (Ucn1) and CRH-receptor (CRH-R1/CRH-R2) expression were examined using quantitative-RT-PCR and immunohistochemistry in nonpregnant and pregnant women (intrauterine, IUP; ectopic pregnancy, EP). Tubal Ucn1 mRNA expression was higher in luteal compared to follicular phase (P<0.01) and equivalent to follicular phase in FT from EP. Tubal CRH-R1/CRH-R2 mRNA was lower in luteal phase (P<0.05) and in FT from EP compared to follicular phase (P<0.01). Ucn1 mRNA was lower in endometrium from EP compared to IUP (P<0.05). CRH-R1 mRNA was higher in endometrium from EP compared to viable IUP (P<0.05). No differences were observed in CRH-R2 expression. CRH-R1 protein was primarily localised to epithelium of FT and endometrium. Quantitative analysis of tubal CRH-R1 protein expression reflected that seen at the mRNA level but endometrial expression was equivocal. The demonstration of attenuated tubal/endometrial Ucn1/CRH-R expression in EP further supports a role of the CRH-family in embryo implantation.

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“…Expression and role of peptides, proteins and growth factors in the pathogenesis of endometriosis ing the concept that ucn-1 may be involved in implantation and establishment of pregnancy (7). a.…”

Section: Introductionmentioning

confidence: 99%

Expression and Role of Peptides, Proteins and Growth Factors in the Pathogenesis of Endometriosis

Florio

Pinzauti

Altomare

et al. 2009

Journal of Endometriosis

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Growing evidence is demonstrating that several peptides (corticotrophin-releasing factor, urocortins, ghrelin), proteins (leptin, adiponectin) and growth factors (vascular endothelial growth factor; epidermal growth factor family of growth factors and receptors, fibroblast growth factor, insulin like growth factor and insulin like growth factor-binding proteins, transforming growth factor-β and, activin A and related proteins) are expressed in endometriotic implants, and locally play a relevant role in affecting the biological mechanisms leading to endometriosis. They establish a complex network of interactions by which they are therefore able to stimulate angiogenesis, inflammatory cell recruitment and reaction, the growth of endometriotic tissue and its survival through the modulation of the narrow immune system. This review will evaluate the role played by several regulatory peptides, proteins and growth factors in affecting endometrial physiology and the putative mechanisms advocated to explain endometriosis (angiogenesis, cellular and humoral immunity, inflammatory response, endometrial cell proliferation, activation, motility, adhesion and invasion).

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Evaluation of Endometrial Urocortin Secretion for Prediction of Pregnancy after Intrauterine Insemination

Cited by 13 publications

References 29 publications

Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome

Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome

Attenuated Tubal and Endometrial Urocortin 1 and Corticotropin-Releasing Hormone Receptor Expression in Ectopic Pregnancy

Expression and Role of Peptides, Proteins and Growth Factors in the Pathogenesis of Endometriosis

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