Evaluation of endometrial receptivity by measuring HOXA-10, HOXA-11, and leukemia inhibitory factor expression in patients with polycystic ovary syndrome

Kara, Mustafa; Özcan, Seda Sabah; Aran, Turhan; Kara, Özlem; Yılmaz, Neziha

doi:10.4103/gmit.gmit_112_18

Cited by 34 publications

(18 citation statements)

References 34 publications

(31 reference statements)

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“…These findings are in accordance with our results showing significantly higher E2 serum levels along with lower expression of Lif in the uterus of Gly and GBH-exposed females exhibiting lower implantation rates (31). Human clinical evidence further supports the requirement of Hoxa10 and Lif for uterine receptivity, based on the association between defective expression of both genes and recurrent implantation failures, infertility, and gynecological disorders including endometriosis, and polycystic ovary syndrome in women (148)(149)(150)(151)(152).…”

Section: Hormonal Molecular and Epigenetic Alterations Associated With Implantation Failuressupporting

confidence: 91%

Glyphosate Herbicide: Reproductive Outcomes and Multigenerational Effects

et al. 2021

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Glyphosate base herbicides (GBHs) are the most widely applied pesticides in the world and are mainly used in association with GBH-tolerant crop varieties. Indiscriminate and negligent use of GBHs has promoted the emergence of glyphosate resistant weeds, and consequently the rise in the use of these herbicides. Glyphosate, the active ingredient of all GBHs, is combined with other chemicals known as co-formulants that enhance the herbicide action. Nowadays, the safety of glyphosate and its formulations remain to be a controversial issue, as evidence is not conclusive whether the adverse effects are caused by GBH or glyphosate, and little is known about the contribution of co-formulants to the toxicity of herbicides. Currently, alarmingly increased levels of glyphosate have been detected in different environmental matrixes and in foodstuff, becoming an issue of social concern. Some in vitro and in vivo studies have shown that glyphosate and its formulations exhibit estrogen-like properties, and growing evidence has indicated they may disrupt normal endocrine function, with adverse consequences for reproductive health. Moreover, multigenerational effects have been reported and epigenetic mechanisms have been proved to be involved in the alterations induced by the herbicide. In this review, we provide an overview of: i) the routes and levels of human exposure to GBHs, ii) the potential estrogenic effects of glyphosate and GBHs in cell culture and animal models, iii) their long-term effects on female fertility and mechanisms of action, and iv) the consequences on health of successive generations.

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Section: Hormonal Molecular and Epigenetic Alterations Associated With Implantation Failuressupporting

confidence: 91%

Glyphosate Herbicide: Reproductive Outcomes and Multigenerational Effects

et al. 2021

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“…The decreased endometrium receptivity in PCOS might be caused by the noticeable imbalance of key proteins (or molecules) and signal cascades in the endometrial tissue (11). Among them, the expression of Integrin ανβ3, HOXA10, COX-2, and LIF in endometrium was different (9,12,13). Improving endometrium receptivity was reported to improve infertility in women with PCOS (14).…”

Section: Introductionmentioning

confidence: 99%

β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota

Cao

Huang

et al. 2021

Front. Nutr.

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Background: Polycystic ovary syndrome (PCOS), one of the most common endocrine diseases in women of childbearing age, has been found to be accompanied by changes in the gut microbiota. The Bu Shen Yang Xue formula (BSYXF) is a traditional Chinese medicine widely used for the treatment of PCOS. This study aimed to investigate whether the protective effects of β-sitosterol, the main active ingredient of BSYXF, on PCOS was mediated by regulating gut microbiota.Methods: The presence of β-sitosterol in BSYXF was detected by liquid chromatography-mass spectrometry. The PCOS-like mouse model was induced by dehydroepiandrosterone. The fecal supernatant of β-sitosterol-treated mice was prepared for fecal microbiota transplantation (FMT). Body weight and wet weight of the uterus and ovary of the mice were recorded for organ index calculation. Hematoxylin and eosin stain was used to assess the endometrial morphology and microenvironment changes. Expression of endometrial receptivity markers cyclooxygenase-2 (COX-2), Integrin ανβ3, leukemia inhibitory factor (LIF), and homeobox A10 (HOXA10) in the endometrium were determined by immunohistochemistry and western blot analysis. Enzyme-linked immunosorbent assay was employed to detect the expression of follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), and testosterone (T) in the serum. The diversity of gut microbiota was examined by 16S rDNA gene sequencing.Results: With the treatment of β-sitosterol and β-sitosterol-FMT, the uterine index of PCOS-like mice increased, the ovarian index decreased, levels of COX-2, LH and T decreased, and levels of Integrin ανβ3, LIF, HOXA10, FSH, and P increased. Under β-sitosterol treatment, the structure of the gut microbiota in PCOS-like mice was also changed.Conclusion: β-sitosterol regulates the endometrial receptivity of PCOS and harmonizes the sex hormone balance, which may be related to the changes in the structure and composition of gut microbiota, thus affecting the pathological process of PCOS.

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“…Sampling and analysis of the endometria of PCOS patients indicate that the expression of HOXA-10 and HOXA-11 genes is reduced, and endometrial decidualization is prevented. Consequently, endometrial receptivity is impaired, which can lead to infertility in women with PCOS [ 36 ]. Meanwhile, PCOS patients exhibit progesterone resistance, thereby reducing the expression of progesterone-dependent HOXA-10 and HOXA-11.…”

Section: Characteristics Of Endometrial Receptivity Markers In Pcos Patients During the Implantation Windowmentioning

confidence: 99%

Research progress of endometrial receptivity in patients with polycystic ovary syndrome: a systematic review

Bai

Zheng

et al. 2021

Reprod Biol Endocrinol

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Polycystic ovary syndrome (PCOS) is a neuroendocrine heterogeneous disease that frequently occurs in women of reproductive age, causing serious damage to the fertility, quality of life, and physical and mental health of patients. The current studies have proved that satisfactory endometrial receptivity is one of the conditions that must be met during the process of spermatovum position, adhesion and invasion, as well as the subsequent blastocyst division and embryo development. Women with PCOS may suffer a series of pathological processes such as changes in the expression levels of hormones and related receptors, imbalances in the proportion of miscellaneous cytokines, insulin resistance, low-grade chronic inflammation and endometrial morphological changes, which will damage endometrial receptivity from various aspects and obstruct fertilized egg nidation and embryonic development, thus causing adverse reproductive health events including infertility and abortion. This article reviews the research progress about characteristics and related influencing factors of endometrial receptivity in PCOS patients.

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Evaluation of endometrial receptivity by measuring HOXA-10, HOXA-11, and leukemia inhibitory factor expression in patients with polycystic ovary syndrome

Cited by 34 publications

References 34 publications

Glyphosate Herbicide: Reproductive Outcomes and Multigenerational Effects

Glyphosate Herbicide: Reproductive Outcomes and Multigenerational Effects

β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota

Research progress of endometrial receptivity in patients with polycystic ovary syndrome: a systematic review

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