Evaluation of embryo aneuploidy (PGT-A) and endometrial receptivity (ERA) testing in patients with recurrent implantation failure in ICSI cycles

Fodina, Violeta; Dudorova, Alesja; Ērenpreiss, Juris

doi:10.1080/09513590.2021.2006466

Cited by 21 publications

(14 citation statements)

References 25 publications

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“…Significantly higher pregnancy rates were reported in the pET group instructed by the commercial Endometrial Receptivity Array (ERA) test, and rescue of non-receptive patients by pET in a displaced WOI resulted in similar clinical outcomes to controls (25,(31)(32)(33)(34). Other investigators, however, considered the value was rather limited when applied ERA in ICSI cycles, first embryo transfer cycles, and in good prognosis patients considering the current level of development (26,30,(35)(36)(37)(38). Based on RNA-seq technology while aiming to improve the diagnostic tool, especially for Chinese population, we constructed an RNA-seq based endometrial receptivity test (rsERT) in a previous study and validated its clinical value in RIF patients (29).…”

Section: Introductionmentioning

confidence: 99%

The RNA-seq based endometrial receptivity test (rsERT) compared to pinopode: A better diagnostic tool for endometrial receptivity for patients with recurrent implantation failure in Chinese population

Chen

Zhang

et al. 2022

Front. Endocrinol.

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Displaced window of implantation (WOI) is one of the endometrial origins that accounts for implantation failure, especially for patients with recurrent implantation failure (RIF), yet no standard diagnostic tool has been recognized. The study consists of two parts, aiming to compare the concordance and efficacy of the diagnostic tools, the newly developed RNA-seq based endometrial receptivity test (rsERT) to the conventional pinopode, in diagnosing WOI and guiding personalized embryo transfer (pET). With the same group of RIF patients, the rsERT diagnosed 32 patients (65.31%) with normal WOIs, and most of the displacements were advancements (30.61%). While according to pinopode, only 14 patients (28.57%) were found with normal WOIs, and most patients (63.27%) presented delayed growth patterns. After conducting pET, patients in the rsERT group had higher successful pregnancy rates while requiring fewer ET cycles (50.00% vs. 16.67%, p=0.001). The study proved poor consistency between the diagnostic tools of endometrial receptivity based on cellular structure and gene profiling, and it supported rsERT as a reliable tool with potential clinical value.

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Section: Introductionmentioning

confidence: 99%

The RNA-seq based endometrial receptivity test (rsERT) compared to pinopode: A better diagnostic tool for endometrial receptivity for patients with recurrent implantation failure in Chinese population

Chen

Zhang

et al. 2022

Front. Endocrinol.

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“…In previous studies, evidence supporting the efficacy of this approach in managing RIF is also insufficient, there were different views on the clinical value of PGT-A for patients with RIF. Fodina and colleagues study the RIF patients whose median age were 35 years, the PGT-A group showed statistically significant higher chance in achieving biochemical and clinical pregnancy (8). In Cozzolina's study, moderate RIF patients were received at least three embryos transferred in different single embryo transfers (SET) without achieving implantation.…”

Section: Introductionmentioning

confidence: 99%

Is it necessary for young patients with recurrent implantation failure to undergo preimplantation genetic testing for aneuploidy?

Guan

et al. 2023

Front. Endocrinol.

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ObjectiveTo determine whether preimplantation genetic testing for aneuploidy (PGT-A) can improve the pregnancy outcomes of patients aged under 38 years who have a history of recurrent implantation failure(RIF).DesignRetrospective cohort study.MethodsWe retrospectively studied the pregnancy outcomes of RIF patients aged under 38 years from January 2017 to December 2021.178 patients were divided into two groups according to whether they underwent PGT-A: the PGT-A group(n=59)and the control group(n=119).In the PGT-A group, we compared the euploidy rate of the different quality and developmental rate blastocysts. In both groups,the patients were the first frozen-thaw single blastocysts transfer after the diagnosis of RIF. Among the pregnancy outcomes, the clinical pregnancy rate was assessed as the primary outcome. The spontaneous abortion rate and ongoing pregnancy rate were the secondry outcomes. The generalized estimation equation was used to adjust for the blastocysts derived from the same patients. Multivariate logistic analysis models were used to compare the pregnancy outcomes between the two groups.ResultsIn the PGT-A group, 293 blastocysts obtained from59 patients underwent PGT-A. The proportions of euploidy, aneuploidy and mosaic blastocysts were 56.31%, 25.60% and 18.09%, respectively. A comparison of the euploidy rates of different quality blastocysts showed that the rate of good-quality blastocysts was significantly higher than that of poor-quality blastocysts (67.66% vs 46.88%; odds ratio [OR], 2.203; 95%confidence interval[CI], 0.943–3.612; P=0.002). However, no significant difference was observed in the different developmental rates blastocysts. Compared with Day 5 blastocysts, the euploidy rates of Day 6 and Day 7 blastocysts were not significantly different(61.54%vs51.91%; OR,0.945; 95%CI, 0.445–2.010; P=0.884; and 61.54%vs47.37%; OR, 1.106; 95%CI, 0.774–1.578; P=0.581, respectively).As for the pregnancy outcomes, the clinical pregnancy rate was significantly increase after the use of PGT-A compared with the control group(71.19%vs56.30%; OR, 0.538; 95%CI, 0.262–1.104; P=0.039). However, the spontaneous abortion rates and ongoing pregnancy rates were not significantly different between the control and PGT-A groups (21.43% vs 19.40%; aOR,0.727; 95%CI,0.271–1.945; P=0.525; and55.93% vs 45.38%; aOR, 0.649; 95%CI, 0.329–1.283; P = 0.214,respectively).ConclusionPGT-A improved the clinical pregnancy rate after blastocyst transfer in RIF patients aged under 38 years.

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“…Numerous studies have reported a certain period of endometrial maturation called the Window Of Implantation (WOI), which starts on Day 19 or 20 of the menstrual period and lasts 4-5 days. During the WOI the blastocyst can attach to the endometrial epithelial cells and subsequently proceed to invade the endometrial stroma; this process is called endometrial receptivity [4,5]. In recent years, the optimal timing of the WOI can be determined through Endometrial Receptivity Analysis (ERA).…”

Section: Introductionmentioning

confidence: 99%

“…Until recently, many studies have investigated the effectiveness of ERA and reported that pET improves IVF success rate [7][8][9]. On the other hand, some studies have reported that ERA did not improve the ongoing pregnancy rate in good prognosis patients or in PGT cycles [10,11]. Similarly, a recent meta-analysis reported that an ERA showed no significant improvement in the IVF outcomes except in the LBR for patients undergoing the first IVF cycle [12].…”

Section: Introductionmentioning

confidence: 99%

Differences in Implantation Timing After Embryo Transfer Based on Embryo Characteristics

2023

Gynecol Obstet Open Acc

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Objective: To evaluate the differences in implantation timing after Embryo Transfer (ET) based on embryo characteristics. Patients: This retrospective study evaluated cases of 7,269 frozen-thawed ET cycles using single blastocysts that resulted in single live birth. The implantation timings after ET were speculated by a calculation formula made for the dynamics of serum human chorionic gonadotropin (HCG) changes. The patients were divided into two groups based on the initial serum HCG (10 days after ET): high-HCG group (HCG > 800) and low-HCG group (HCG < 50). Results: The average estimated implantation timing (HCG = 0) was 3.3 days after ET. The serum HCG levels after logarithmic conversion (logHCG) changed in parallel regardless of the starting HCG levels. The estimated implantation timing varied approximately five days (2.5-7.5 days after ET) based on the starting HCG levels. The ratios of matured blastocysts (3 or higher in Gardner's grading scale) were significantly higher in the high-HCG group (90.9%) than in the low-HCG group (47.4%). Similarly, the average grade of blastocysts was higher in the high-HCG group than in the low-HCG group (4.72 vs. 3.15, p < 0.01). Conclusions: The implantation timing after ET varies based on embryo characteristics.

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Evaluation of embryo aneuploidy (PGT-A) and endometrial receptivity (ERA) testing in patients with recurrent implantation failure in ICSI cycles

Cited by 21 publications

References 25 publications

The RNA-seq based endometrial receptivity test (rsERT) compared to pinopode: A better diagnostic tool for endometrial receptivity for patients with recurrent implantation failure in Chinese population

The RNA-seq based endometrial receptivity test (rsERT) compared to pinopode: A better diagnostic tool for endometrial receptivity for patients with recurrent implantation failure in Chinese population

Is it necessary for young patients with recurrent implantation failure to undergo preimplantation genetic testing for aneuploidy?

Differences in Implantation Timing After Embryo Transfer Based on Embryo Characteristics

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