Evaluation of Electronic Red Blood Cell Counter

Brecher, George; Schneiderman, Marvin A.; Williams, George Z.

doi:10.1093/ajcp/26.12.1439

Cited by 155 publications

(46 citation statements)

References 4 publications

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“…The volume profile of cells in suspension was analyzed by an electronic cell counter (25,26) (Coulter Counter ZM Analyzer; aperture, 100 m, 1/current, 1/4; 1/gain, 1/1) connected to a pulse height analyzer (Coulter Channelyzer Model 256; Beckman Coulter, Miami, FL). Calibration of the system to obtain absolute volumes was performed daily using 10-m microspheres (Beckman Coulter).…”

Section: Electronic Cell Volumementioning

confidence: 99%

Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin

West

et al. 2001

The Journal of Immunology

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The systemic immune response is a dynamic process involving the trafficking of lymphocytes from the Ag-stimulated lymph node to the peripheral tissue. Studies in sheep have demonstrated several phases of cell output in the efferent lymph after Ag stimulation. When skin contact sensitizers are used as Ag, the efferent lymph cell output peaks ∼96 h after Ag stimulation and is temporally associated with the recruitment of cells into the skin. To investigate the relative contribution of this high-output phase of efferent lymphocytes to lymphocytic inflammation in the skin, we used a common contact sensitizer 2-phenyl-4-ethoxymethylene-5-oxazolone (oxazolone) to stimulate the skin and draining prescapular lymph node of adult sheep. The efferent lymph ducts draining the Ag-stimulated and contralateral control lymph nodes were cannulated throughout the experimental period. The lymphocytes leaving the lymph nodes during the 72-h period before maximum infiltration were differentially labeled with fluorescent tracers, reinjected into the arterial circulation, and tracked to the site of Ag stimulation. Quantitative tissue cytometry of the skin at the conclusion of the injection period (96 h after Ag stimulation) demonstrated more migratory cells derived from the Ag-stimulated lymph node than the contralateral control (median 18.5 vs 15.5 per field; p < 0.05). However, when corrected for total cell output of the lymph node, the Ag-stimulated migratory cells were 3.8-fold more prevalent in the skin than the contralateral control cells. These results suggest that the in situ immune response generally mirrors the frequency of recruitable lymphocytes in the peripheral blood.

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Section: Electronic Cell Volumementioning

confidence: 99%

Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin

West

et al. 2001

The Journal of Immunology

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“…For the measurement of the volumes of Prymnesium organisms, the Coulter Particle Counter, Model A (Coulter Electronics, Chicago, Illinois, U.S.A.) with an orifice of 100 p was used. This instrument provides a means to determine size distribution of cell populations and has already proved extremely useful in the counting and sizing of erythrocytes (Mattern, Brackett & Olson, 1957), in the estimation of the volumes of cells in tissue cultures (Kuchler & Merchant, 1958; Brecher, Schneiderman & Williams, 1956) and of bacteria (Kubitschek, 1958;Lark & Lark, 1960).…”

Section: Introductionmentioning

confidence: 99%

The Mechanism of Lysis of Prymnesium parvum by Weak Electrolytes

Shilo¹

1962

Journal of General Microbiology

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SUMMARYOrganisms of the phytoflagellate Pryrnnesium parvum Carter undergo swelling and lysis in the presence of ammonia or acetic acid. The lytic activity was pH-dependent and increased as the concentration of undissociated weak electrolyte in the suspension medium was increased. The kinetics of swelling were followed with the aid of an electronic particle counter and by microscopic examination. In the presence of ammonia or acetic acid, the rate of swelling as well as the final volume of the Prymnesium organisms were a function of external osmotic pressure and of temperature. It is suggested that the swelling and lysis are osmotic in nature and depend on the intracellular accumulation of weak electrolytes by a 'pump' driven by differences between environmental and intracellular pH values. The similarities between this phenomenon and concentration of weak electrolytes in mammalian erythrocytes and other cells is discussed. Differences in the morphology and lysis of Prymnesium by ammonia and acetic acid were found which suggest the existence of intracellular compartments maintained at different pH values.

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“…5P 3 and --2,503. The shape of the function relating threshold tL particle volume for particles smallhr than --0.75p 3 it is necessary to distinguish between the ability of the Coulter Counter to respond to the passage through its aperture of particles smaller than -O.75p3 in volume and the ability of the Counter to proviPe, without further investigation of its properties, an estrmate of the volume diatribution -feh particle3.…”

Section: Discussionmentioning

confidence: 96%

“…Although passage of liquids through a cellulose filter (0.45p pore d!a=,er) is imsida"a-d iufficleat to remove almost all varietiee tif nonviral living organisms, 3 2 it was observed that t4hs was not sufficient to remove particles that interfered with the work reported here, hence the vrocedure adopted.…”

Section: Asi'i Iw II 25mentioning

confidence: 94%

Coulter Counter Theory and Coincidence Count Corrections: Modifications and Additions for Work With Spheres About One Micron in Diameter

Mercer¹

1965

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Evaluation of Electronic Red Blood Cell Counter

Cited by 155 publications

References 4 publications

Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin

Stochastic Regulation of Cell Migration from the Efferent Lymph to Oxazolone-Stimulated Skin

The Mechanism of Lysis of Prymnesium parvum by Weak Electrolytes

Coulter Counter Theory and Coincidence Count Corrections: Modifications and Additions for Work With Spheres About One Micron in Diameter

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