Evaluation of EGFR protein expression by immunohistochemistry using H-score and the magnification rule: Re-analysis of the SATURN study

Mazières, Julien; Brugger, Wolfram; Cappuzzo, Federico; Middel, Peter; Frosch, Alice; Bara, Ilze; Klingelschmitt, Gaëlle; Klughammer, Barbara

doi:10.1016/j.lungcan.2013.07.016

Cited by 81 publications

(69 citation statements)

References 24 publications

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“…Both a membranous immunostaining signal on the tumour cell surface and cytoplasmic staining within the tumour cells were recorded. Also, the intensity of membranous staining (weak, moderate, strong) and percentage of positive tumour cells were evaluated in order to calculate an H‐score (intensity × percentage; range 0–300) …”

Section: Methodsmentioning

confidence: 99%

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Tumour‐infiltrating lymphocytes and expression of programmed death ligand 1 (PD‐L1) in melanoma brain metastases

et al. 2014

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Section: Methodsmentioning

confidence: 99%

“…Also, the intensity of membranous staining (weak, moderate, strong) and percentage of positive tumour cells were evaluated in order to calculate an H-score (intensity 9 percentage; range 0-300). 17…”

Section: Pd-l1mentioning

confidence: 99%

Tumour‐infiltrating lymphocytes and expression of programmed death ligand 1 (PD‐L1) in melanoma brain metastases

et al. 2014

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“…Additionally, the percentage of stained cells was estimated for each intensity. The percentage of CD164-stained cells for each intensity was multiplied by the corresponding intensity score to obtain an immunostaining score (H-score) that ranged between 0 and 300 (26).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of CD164 in oral cavity squamous cell carcinoma predicts a favourable prognosis

Chen

Chan

et al. 2017

Oncol Lett

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Abstract. The aim of the present study was to investigate the association between cluster of differentiation (CD) 164 expression with clinicopathological parameters and prognosis among patients with oral cavity squamous cell carcinoma (OSCC). The present study retrospectively reviewed 70 patients with OSCC who underwent curative primary surgery. A number of patients subsequently received postoperative chemoradiotherapy although the specimens were not exposed to radiation or chemotherapy prior to anti-CD164 antibody immunohistochemical staining. CD164 overexpression was arbitrarily defined as exhibiting an H-score of ≥120. Univariate and multivariate analyses were performed for sex, age, American Joint Committee on Cancer stage, tumour location, histological grade, surgical margin and H-score. The 5-year overall survival rate was 54.4% and the median follow-up time was 46 months for surviving patients. Univariate analyses revealed that a low overall survival rate was associated with advanced-stage disease (P<0.001), buccogingival tumour location (P=0.038) and a CD164 H-score of <120 (P=0.016). Multivariate Cox's regression analyses revealed that poor overall survival rate was associated with advanced-stage disease (P=0.001) and a CD164 H-score of <120 (P=0.04). CD164 overexpression in OSCC was associated with favourable survival rate. Thus, CD164 expression may be a clinically useful predictor of prognosis in patients with OSCC.

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“…To evaluate relative immune-intensity in lung adenocarcinoma, immunoreactivity was also evaluated by H scoring system [40]. Briefly, staining carcinoma cells were further classified into the strongly or weakly positive cells, and H scores were subsequently generated by adding together 3x % strongly stained cells, 2x % moderate stained cells 1x % weakly stained cells, and 0x % negative cells.…”

Section: Methodsmentioning

confidence: 99%

Orai3 is a predictive marker of metastasis and survival in resectable lung adenocarcinoma

et al. 2016

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Orai3 channel has emerged as important player in malignant transformation. Indeed, its expression is increased in cancer and favors cell proliferation and survival by permitting calcium influx. In this study, Orai3 was overexpressed in lung adenocarcinoma as compared to their matched non-tumour samples and was associated with tumoural aggressiveness. Moreover, its expression was associated with estrogen receptor alpha (ERα) expression and visceral pleural invasion in multivariate analysis. Furthermore, both the overall survival (OS) median and the metastasis free survival (MFS) median of tumors with high Orai3 expression were lower than in low Orai3 expression regardless of cancer stage (35.01 months vs. 51.11 months for OS and 46.01 months vs. 62.04 months for MFS). In conclusion, Orai3 protein level constitutes an independent prognostic marker in lung adenocarcinoma, and a novel prognostic marker that could help selecting the patients with worst prognosis to be treated with adjuvant chemotherapy in resectable stage.

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Evaluation of EGFR protein expression by immunohistochemistry using H-score and the magnification rule: Re-analysis of the SATURN study

Abstract: These data suggest that EGFR IHC does not have value as a marker to predict erlotinib benefit in the first-line maintenance setting for advanced NSCLC.

Cited by 81 publications

References 24 publications

Tumour‐infiltrating lymphocytes and expression of programmed death ligand 1 (PD‐L1) in melanoma brain metastases

Tumour‐infiltrating lymphocytes and expression of programmed death ligand 1 (PD‐L1) in melanoma brain metastases

Overexpression of CD164 in oral cavity squamous cell carcinoma predicts a favourable prognosis

Orai3 is a predictive marker of metastasis and survival in resectable lung adenocarcinoma

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