Evaluation of efficacy of non-thermal atmospheric pressure plasma in treatment of periodontitis: a randomized controlled clinical trial

Küçük, Diğdem; Savran, Levent; Ercan, Utku Kürşat; Yarali, Ziysan Buse; Karaman, Ozan; Kantarcı, Alpdoğan; Sağlam, Mehmet; Köseoğlu, Serhat

doi:10.1007/s00784-019-03187-2

Cited by 25 publications

(22 citation statements)

References 51 publications

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“…However, no significant difference was found between the groups in terms of gingival crevicular fluid biomarkers (IL-1β, VEGF, and IL-10) and probing depth. Although there are many data in the literature showing that reactive species in NTAP increase the release of various growth factors and cytokines, such a result in this study was explained by the fact that scaling and root planning (SRP) masked the effect of NTAP [41]. In our study, the application of NTAP to the ligatured teeth without an alternative therapy method may have caused the difference between the groups to emerge more clearly.…”

Section: Discussionmentioning

confidence: 62%

“…In addition, the most obvious advantage that is accepted in this regard is that plasma achieves this effect without damaging human cells [55]. Recently, a clinical study indicated that singletime NTAP application together with non-surgical periodontal treatment provided additional clinical attachment level gains and better elimination of putative periodontopathogens and reduced their recolonization [41]. These findings support the primary antiseptic effect of NTAP; in this way, NTAP may prevent the onset of periodontal disease.…”

Section: Discussionmentioning

confidence: 85%

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Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

2021

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Objectives The inhibition of bone destruction is one of the main goals of periodontitis treatment. The aim of this study was to investigate the protective effects of non-thermal atmospheric plasma (NTAP) on alveolar bone loss radiographically, histomorphometrically, and histologically in experimental periodontitis in rats. Materials and methods A total of twenty-eight rats were randomly divided into three groups: control group (CG) (n = 8), periodontitis group (PG) (n = 10), and NTAP group (NTAPG) (n = 10). In PG and NTAPG, experimental periodontitis was created with ligating. The kINPen 11 plasma jet was applied around the ligatured teeth in NTAPG. The samples from each group were radiographically assessed with microcomputed tomography (micro-CT); then, histological (presence of osteoclasts and inflammatory cells) and immunohistochemical (immunoreactive of OCN and ALP) findings were compared. ResultsThe results revealed a significant increase in alveolar bone loss in the PG compared with CG and NTAPG (p < 0.05). Inflammation, alveolar resorption, and cement damage were reduced significantly in the group treated with NTAP compared to the PG (p < 0.05). Significantly higher levels of osteoclasts were detected in the PG in comparison with both CG and NTAPG (p < 0.05). The lowest osteocalcin and ALP values were determined in PG, and the differences between PG and both groups were also significant (p < 0.05). Conclusion Within the limitations of the present study, we can say that NTAP may enhance the bone remodeling process by inhibiting inflammation and preventing alveolar bone destruction.Clinical relevance NTAP has clinical potential for accelerating and treating periodontitis with the inflammatory response modulation, osteoblast differentiation, and alveolar bone loss reduction.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 85%

Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

2021

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“…Furthermore, levels of P. gingivalis and Tannerella forsythia were significantly decreased compared to the control [93]. In the only RCT published, patients were treated with adjunctive CAP and showed significant more attachment level gain in severe periodontal pockets and a reduced load of periopathogens compared to conventional treatment 3 months after therapy [94].…”

Section: Discussionmentioning

confidence: 96%

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

et al. 2021

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Interest in the application of cold atmospheric plasma (CAP) in the medical field has been increasing. Indications in dentistry are surface modifications and antimicrobial interventions. The antimicrobial effect of CAP is mainly attributed to the generation of reactive oxygen and reactive nitrogen species. The aim of this article is to systematically review the available evidence from in-vitro studies on the antimicrobial effect of CAP on dental pathogens. A database search was performed (PubMed, Embase, Scopus). Data concerning the device parameters, experimental set-ups and microbial cultivation were extracted. The quality of the studies was evaluated using a newly designed assessment tool. 55 studies were included (quality score 31–92%). The reduction factors varied strongly among the publications although clusters could be identified between groups of set pathogen, working gases, and treatment time intervals. A time-dependent increase of the antimicrobial effect was observed throughout the studies. CAP may be a promising alternative for antimicrobial treatment in a clinically feasible application time. The introduced standardized protocol is able to compare the outcome and quality of in-vitro studies. Further studies, including multi-species biofilm models, are needed to specify the application parameters of CAP before CAP should be tested in randomized clinical trials.

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“…Periodontal therapies are well established and have demonstrated good results, though still there are limitations regarding the traditional root planing and scaling such as short-term recolonization and no lost tissue gain, even when surgical treatment is applied [ 7 ]. Moreover, antibiotic therapy, commonly prescribed during the treatment, has undesirable side effects.…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease

et al. 2021

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Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.

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Evaluation of efficacy of non-thermal atmospheric pressure plasma in treatment of periodontitis: a randomized controlled clinical trial

Cited by 25 publications

References 51 publications

Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease

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