Evaluation of Efficacy of Bacteriophage CJø07 against Salmonella enteritidis Infection in the SPF Chicks

Abstract

In the present study we report in vivo inhibitory potential of single strain of bacteriophage (CJø07) in day-old SPF chicks experimentally infected with Salmonella enteritidis (SE). The bacteriophages prepared by feed additives and drinking water were given to chicks for 20 days starting prior 10 days before challenge with SE. Chicks were euthanized at 10 days after challenge for quantitative salmonella isolation from intestine and determination of environmental contamination level of salmonella. Bacteriophage therapy as additives in feed and drinking water resulted in significant inhibition of the SE replication in intestines of SPF chickens (P<0.05). In addition, environmental contamination by SE fecal shedding was decreased in bacteriophage-treated birds. Therefore, bacteriophage CJø07 examined in this study may be a plausible alternative to antibiotics for the reduction of salmonella infection both in poultry.

Evaluation of Anti-&lt;italic&gt;SE&lt;/italic&gt; Bacteriophage as Feed Additives to Prevent &lt;italic&gt;Salmonella enteritidis&lt;/italic&gt; (SE) in Broiler

“…When bacteriophage is utilized as a feed additive instead of antibiotics in animal feed then its inhibitive effect on harmful bacteria replication in the gastrointestinal tract can be expected ( Yongsheng et al, 2008 ). Lim et al (2010) demonstrated a high level of bacteriophage was excreted via feces when bacteriophage was supplemented to broilers diet. Moreover, their study concluded that broiler growth was improved by supplementation of bacteriophage due to the fact that bacteriophage inhibited the growth of Salmonella enteritidis in the gastrointestinal tract of broilers.…”

Prevalence and Characteristics of Salmonella spp. Isolated from Raw Chicken Meat in the Republic of Korea

“…Many foodborne illness outbreaks of harmful bacteria in foods are reported each year, and these human health-threatening incidences have had various patterns [ 1 ]. Across the globe, outbreaks of food poisoning tend to occur in groups and become larger while the key pathogenic organisms causing food poisoning include Salmonella spp., Escherichia coli , Staphylococcus aureus , Clostridium perfringens , and Vibrio parahaemolyticus [ 2 , 3 ].…”

The Efficiency of Bacteriophages Against Salmonella Typhimurium Infection in Native Noi Broilers

“…Apart from being used as therapeutic bioagents to eradicate bacteria, bacteriophages have been used as feed additives to prevent pathogens, resulting in productivity advantage. For example, in experiments using diets containing phages against S. Gallinarum, S. Typhimurium, and S. Enteritidis, where broiler chickens were raised under normal physiological conditions (without bacterial challenge), improvements in body weight and FCR were observed (Lim et al, 2010;Kim et al, 2014;Wójcik et al, 2020). This is due to the fact that the bacteriophage inhibits the growth of bacteria in the broilers' gastrointestinal tract (Lim et al, 2010), and the increase in body weight may be related to an increase in feed intake (Upadhaya et al, 2021).…”

“…For example, in experiments using diets containing phages against S. Gallinarum, S. Typhimurium, and S. Enteritidis, where broiler chickens were raised under normal physiological conditions (without bacterial challenge), improvements in body weight and FCR were observed (Lim et al, 2010;Kim et al, 2014;Wójcik et al, 2020). This is due to the fact that the bacteriophage inhibits the growth of bacteria in the broilers' gastrointestinal tract (Lim et al, 2010), and the increase in body weight may be related to an increase in feed intake (Upadhaya et al, 2021). However, not all cases of phage therapy or phage supplementation in diets resulted in enhanced broiler production traits.…”