Evaluation of effectiveness of an innovative semen extender (Formula^®) comparing with a traditional extender (Lepus^®) for artificial insemination in rabbits does

Abstract

This study aimed to investigate the preservability and viability of the rabbit spermatozoa diluted in a new semen extender Formula V R in comparison with Lepus V R at 17 C of storage. The main characteristic of the new extender formulation is the use of an enzymatic agent associated to a polysaccharide as energy source precursor, added with gentamycin. During eight trials, ejaculates from 70 bucks were collected and diluted at 1:10 ratio with both the extenders, after 24 h of storage the semen doses were used for the artificial insemination (AI). Aliquots of the semen doses for each trial were stored at 17 C, the total and progressive motility were checked at 0, 4, 12, 18, 24, 36, 48, 60, 72, 84, 96, 108 h of storage. A total of 1267 and 1525 does were inseminated, respectively with Formula V R and Lepus V R . During storage the mean total and progressive motility (77.23% and 72.854%, respectively) were significantly higher for Formula V R (p < .01) and the progressive motility at almost 70% was maintained for at least 60 h vs the 24 h of storage for Lepus V R with significant differences after 12 h of storage (p < .05). The new extender reported a higher pregnancy rate (p < .05) and an average of 9.25 rabbits born per litter vs 8.83 for the traditional extender (p < .05), while the mean of the newborn alive was 9.08 using Formula V R vs 8.51 with Lepus V R (p < .05). In conclusion, the use of Formula V R is recommended for rabbit semen AI programmes. ARTICLE HISTORY

Antibiotics Versus Natural Biomolecules: The Case of In Vitro Induced Bacteriospermia by Enterococcus Faecalis in Rabbit Semen

“…Our results show that among the selected biomolecules, GEN proved to be the best motion-preserving supplement. A similar observation was reported by Bresciani et al [18] who developed a new extender for rabbit semen containing up to 40 mg/mL GEN. Nevertheless, we have to treat our results with caution as Jasko et al [54] emphasized on the harmful effects of GEN on the motility of stallion spermatozoa at concentrations greater than 1 mg/mL.…”

“…The microbiological assessment of the control and experimental samples revealed that all ATBs performed better against the growth of E. faecalis in comparison with the selected natural biomolecules. As expected, and indeed indicated in previous reports, GEN and KAN are considered to be antibacterial substances of choice for semen extenders in rabbits [18,19], stallions [86] and bulls [87]. Nevertheless, the ever-increasing bacterial tolerance and/or resistance towards antibiotics needs to be remembered.…”

“…Because of a varying biological activity of conventional ATBs, and taking into account their potential toxic effects on the bacterial or eukaryotic cell, the concentrations of 300 µg/mL PEN, 1000 µg/mL GEN and 80 µg/mL KAN were selected based on the European Council Directive 90/429/EEC, Annex C2 [20] as well as information gathered from previous scientific reports focused on the preservation possibilities of rabbit ejaculates [18,19,20]. The final doses of ATBs were furthermore validated in our laboratory settings prior to the in vitro experiments.…”

“…The aim of this study was a deep analysis of the efficiency of selected natural biomolecules (RES, QUE, CUR) and antibiotics traditionally used in animal biotechnologies (penicillin-PEN, gentamycin-GEN, kanamycin-KAN) [18,19,20] to decelerate the detrimental processes resulting from a co-culture of rabbit spermatozoa with an uropathogenic bacterium ( Enterococcus faecalis ) isolated from rabbit ejaculates. The methodic steps of this study were focused on a complex assessment of the essential components of the structural integrity and functional activity of male reproductive cells.…”

Artificial insemination of rabbits: current state and practical possibilities of the method

“…Первая среда содержит следующие компоненты: трис(гидроксиметил)аминометан -3,029 г, лимонная кислота -1,676 г, дегидрированная D-глюкоза -1,25 г, стрептомицин -75000 IU, G-пенициллин -166200 IU, вода дистиллированная -до 100 мл; pH -7,14; осмоляльность -299 мОсм/кг. В состав второй среды входят: хлорид натрия -5,690 г, хлорид калия -0,231 г, хлорид кальция 2-водный -0,294, магния хлорид 6-водный -0,081г, натрий фосфорнокислый двузамещенный (гидрофосфат натрия) -0,040 г, натрия гидрокарбонат -2,090 г, натрия пируват -0,022 г, D-глюкоза -0,900 г, молочная кислота -3,680 г, HEPES (4-(2-гидроксиэтил)-1-пиперазин этансульфоновая кислота -буферный агент) -2,380 г стрептомицин -75,000 IU, G-пенициллин -166,200 IU, вода дистиллированная -до 1 л; pH -7,40; осмоляльность -296 мОсм/кг [12,14,15].…”