Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen–glycosaminoglycan biopolymer in a caprine model

Getgood, Alan; Kew, Simon J.; Brooks, Roger A.; Aberman, Harold M.; Simon, Timothy M.; Lynn, Andrew K.; Rushton, Neil

doi:10.1016/j.knee.2011.03.011

Cited by 80 publications

(66 citation statements)

References 30 publications

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“…Its composition is based on a collagen type-I hydroxyapatite gradient. MaioRegen® has demonstrated throughout its clinical studies that its technique is safe and that the clinical results in the short-and medium-term follow-up are efective in a large population of patients [144][145][146][147][148], even atributed beter results than other commercial scafolds ChondroMimetic TM [149] and TruFit CB TM [11,[150][151][152]. Despite the encouraging in vitro results of a wide variety of osteochondral scafolds, most are in the early stages of development.…”

Section: The Eicacy and Safety Of Diferent Treatments Of Cartilage Inmentioning

confidence: 99%

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fuentes-Mera¹,

Camacho²,

Moncada‐Saucedo³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Articular cartilage injuries caused by traumatic/mechanical progressive degeneration result in joint pain, swelling, the consequent loss of joint function, and eventually osteoarthritis. Articular tissue possesses a poor ability to regenerate that further complicates the therapeutic approaches. Mesenchymal stem cells (MSCs) have emerged as a promising alternative treatment. Recently, it has been reported that a wide variety of strategies ranging from merely using cells in the injured area to employ biofunctional substitutes in which cells are harmonizing with scafolding and growth factors to create an engineered cartilage tissue.This chapter reviews the state-of the-art in cartilage tissue engineering focused on tissue engineering approaches designed to recapitulate the native development of cartilage and its tridimensional structure as an osteochondral unit. Since the production of hypertrophied tissue is one of the most critical challenges to overcome in chondral tissue regeneration, here we show new strategies to minimize hypertrophy in cartilage. Finally, the eicacy and safety of diferent treatments of cartilage in current clinical trials will be discussed.While the framework provides new features and beneits concerning the strategies for articular tissue regeneration, this chapter presents a set of tools to improve approaches to orthopedic regenerative medicine based on the use of MSCs.Keywords: MSCs, MSCs-subpopulations, cartilage regeneration, cartilage tissue engineering, hypertrophy © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. IntroductionThe chondrocytes are the only cells found in cartilage. The chondrocytes demonstrate distinctive properties such as being metabolically active in order to maintain the renewal of the extracellular matrix (ECM) by synthesizing collagens, proteoglycans, hyaluronic acid, and glycoproteins. Restoration of the cartilage damage is still challenging for orthopedic medicine due to its poor ability to regenerate [1].Mesenchymal stem cells (MSCs) have potential applications in tissue engineering, and regenerative medicine represents an atractive option for repairing lesions in cartilage. Stem cellbased therapies that harmonize with tissue-engineering technologies, and biomaterials are vital for the continuous advance of cartilage regenerative medicine [2,3].Once the relationship between structure function in normal and damaged tissues is understood and the development of biological substitutes for the repair or regeneration can be reached. To develop a biological substitute, tissue engineering uses scafolds, cells, and growth factors. Each of these elements alone is able to promote tissue regeneration, but composites fabricated in combination would be more efective [4,5].The objective of the present chapter is, therefore, t...

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Section: The Eicacy and Safety Of Diferent Treatments Of Cartilage Inmentioning

confidence: 99%

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fuentes-Mera¹,

Camacho²,

Moncada‐Saucedo³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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“…For example, collagen-glycosaminoglycan phosphate biphasic scaffold were evaluated in caprine femoral condyle and lateral trochlear sulcus osteochondral defects model. After 26 weeks of implantation, both scaffolds provide indications of structural repair [41] .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of titanium based biphasic scaffold using selective laser melting and collagen immersion

Sing¹,

Wang²,

Agarwala³

et al. 2017

ijb

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Fabrication of titanium based biphasic scaffold using selective laser melting and collagen immersion. © 2017 Swee Leong Sing, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/ licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 65 RESEARCH ARTICLEFabrication of titanium based biphasic scaffold using selective laser melting and collagen immersion Abstract: Tissue engineering approaches have been adopted to address challenges in osteochondral tissue regeneration. Single phase scaffolds, which consist of only one single material throughout the whole structure, have been used extensively in these tissue engineering approaches. However, a single phase scaffold is insufficient in providing all the properties required for regeneration and repair of osteochondral defects. Biphasic scaffolds with two distinct phases of titanium/type 1 c ollagen and titanium-tantalum/type 1 collagen were developed for the first time using selective laser melting and collagen infiltration. Observation of the biphasic scaffolds demonstrated continuous interface between the two phases and mechanical characterization of the metallic scaffolds support the feasibility of the newly developed scaffolds for tissue engineering in osteochondral defects. Keywords: elective aser elting, itanium, antalum, ollagen, iphasic caffolds

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“…The goal of these strategies is to use cells, scaffolds and growth factors to regenerate the damaged tissue and thus provide long term relief to the patient (2). While numerous advances have been made in the design of multiphasic scaffolds (3)(4)(5), cell culture protocols (6,7) and growth factor releasing materials (8,9), a number of issues still exist which limit the widespread implementation of these techniques. One of the most prominent of these is promoting the formation of stable bone and cartilage within the osseous and chondral regions of the defect respectively.…”

Section: Introductionmentioning

confidence: 99%

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

O'Reilly

Kelly

2017

Tissue Engineering Part A

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Tissue engineering (TE) has recently emerged as a potential method with which to treat osteochondral defects. In order to improve these strategies, an understanding is required of how the cells within an implanted scaffold interact with their local environment.To this end, the objective of this study was to develop a computational model of an osteochondral defect following the implantation of a tissue engineered scaffold. In particular, the purpose of this was to systematically explore the relationship that scaffold design has on the spatial formation of cartilage and bone within the defect. In this study, tissue formation was predicted using a previously developed algorithm in which cell fate was dependent upon the local oxygen tension and the local mechanical environment. In the first part of this study, the model was updated to include a condition whereby mature cartilage was resistant to both terminal differentiation and vascularisation. The updated model was then tested by using it to predict tissue formation during the spontaneous repair process within an osteochondral defect. Following this, the model was used to simulate an osteochondral defect treated with different variations of a previously developed multiphasic scaffold. By comparing the results of the different simulations it was possible to elucidate a mechanism by which the scaffold promoted robust bone and cartilage formation. In particular, support was provided for a previous hypothesis which proposed that osteochondral defect repair can be enhanced by actively confining angiogenesis to within the subchondral region of the defect for a sustained period of time. Based on the results of this study, it was possible to expand on this by suggesting that this time period be no less than 10 weeks. It is possible to reduce this, however, by pre-culturing MSCs under chondrogenic conditions prior to implantation within the defect.

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Evaluation of early-stage osteochondral defect repair using a biphasic scaffold based on a collagen–glycosaminoglycan biopolymer in a caprine model

Cited by 80 publications

References 30 publications

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Current Applications of Mesenchymal Stem Cells for Cartilage Tissue Engineering

Fabrication of titanium based biphasic scaffold using selective laser melting and collagen immersion

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

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