Evaluation of dried blood spot testing using the Abbott Alinity i

Shepherd, Samantha J.; McLellan, H.G.; Bell, Janet; Gunson, Rory

doi:10.1016/j.jcv.2020.104638

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…The majority of studies ( n = 15) focused on general populations, such as Chinese residents in the United Kingdom and individuals aged 1–39 in the South‐Central coast region of Vietnam 18–31 . Fourteen studies ( n = 14) examined populations with known HBV, HCV and/or HIV status 17,32–43 …”

Section: Resultsmentioning

confidence: 99%

“…[18][19][20][21][22][23][24][25][26][27][28][29][30][31] Fourteen studies (n = 14) examined populations with known HBV, HCV and/or HIV status. 17,[32][33][34][35][36][37][38][39][40][41][42][43] Twelve articles (n = 12) examined patients attending or staying in hospitals, clinics and health centres. 19,[44][45][46][47][48][49][50][51][52][53][54] Eleven studies (n = 11) investigated populations engaged in risky behaviours that increase their susceptibility to blood-borne viruses (BBVs) like HBV.…”

Section: F I G U R Ementioning

confidence: 99%

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The role of dried blood spot tests in the detection of hepatitis B infection: A systematic review

Virtudazo,

Aquino,

Arellano

et al. 2023

Journal of Viral Hepatitis

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Hepatitis B remains a public health problem worldwide despite vaccine availability. Although the existing diagnostic tools help detect the infection, logistics support and limited resources and technologies affect their usefulness and reliability in developing countries. This systematic review evaluated the performance of dried blood spots (DBS) as a collection and storage tool for diagnosing an hepatitis B virus (HBV) infection. A comprehensive search using OVID, Scopus and CINAHL databases was performed to collate articles published up to April 2023 that detected Hepatitis B infections using DBS. Five reviewers independently performed identification, screening, quality assessment and data extraction. A qualitative synthesis of the included studies was conducted. Of the 402 articles, 78 met the inclusion criteria. The results show that most studies focused on populations with known HBV, HCV and/or HIV status. Approximately half (49%) of the included studies utilized the Whatman Protein Saver Card for DBS collection. The DBS samples were then predominantly stored in room temperature conditions. In line with this, storage conditions influenced the concentration and stability of the analyte from the DBS samples, affecting the accuracy of downstream diagnostic methods. ELISA methods, using hepatitis B surface antigen (HBsAg) as an HBV marker, were the most widely used diagnostic tool for detecting HBV infection in DBS samples. The simplicity and cost‐effectiveness of the ELISA technique highlight its potential to be used in low‐resource settings. In line with this, the detection of HBsAg using an ELISA immunoassay had higher sensitivity (85.6%–100%), and specificity (95%–100%) ranges as compared to other target molecules and methods. Although this review only performed a qualitative analysis, DBS offers a promising method for collecting and storing blood samples; however, the standardization of sampling, storing conditions and diagnostic techniques is required to ensure sustainable application.

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Section: Resultsmentioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

The role of dried blood spot tests in the detection of hepatitis B infection: A systematic review

Virtudazo,

Aquino,

Arellano

et al. 2023

Journal of Viral Hepatitis

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“…In this study, 13 anti-HCV detection kits were enrolled: ARCHITECT Anti-HCV (ARCHITECT-Ab; Abbott Japan, Tokyo, Japan) (Yoo et al, 2015;Shepherd et al, 2020;Nam et al, 2021), Alinity i Anti-HCV (Alinity-Ab; Abbott Japan) (Shepherd et al, 2020;Nam et al, 2021), Lumipulse HCV (Lumipulse-Ab; Fujirebio, Tokyo, Japan), Lumipulse Presto HCV (Lumipulse Presto-Ab; Fujirebio), Lumipulse II Ortho HCV (Ortho-Ab; Fujirebio), Lumipulse Presto Ortho HCV (Ortho Presto-Ab; Fujirebio), Elecsys Anti-HCV II (Elecsys-Ab; Roche Diagnostics, Tokyo, Japan) (Yoo et al, 2015;Feng et al, 2016;Xu et al, 2019), Accuraseed HCV [II], (Accuraseed-Ab; Sanyo Chemical Industries, Kyoto, Japan), ADVIA Centaur anti-HCV assay (Centaur-Ab; Siemens Healthcare Diagnostics, Tokyo, Japan) (Dwyer, 2004;Yoo et al, 2015), HISCL Anti-HCV Assay Kit (HISCL-Ab; Sysmex, Kobe, Japan) (Feng et al, 2016), HISCL Anti-HCV II Assay Kit (HISCL II-Ab; Sysmex) (Xu et al, 2019), CL AIA-PACK HCVAb (CL AIA-Ab; TOSOH, Tokyo, Japan), and E Test 'TOSOH' II HCVAb (AIA-Ab; TOSOH). Of these anti-HCV detection kits, Lumipulse-Ab, Lumipulse Presto-Ab, Ortho-Ab, Ortho Presto-Ab, Accuraseed-Ab, HISCL-Ab, and CL AIA-Ab provide classification titers.…”

Section: Evaluation Of Hcv Detection Kitsmentioning

confidence: 99%

Performance Evaluation of In Vitro Screening and Diagnostic Kits for Hepatitis C Virus Infection

Murayama

Momose

Yamada

et al. 2022

Front. Cell. Infect. Microbiol.

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AimA reliable kit with high sensitivity and specificity is indispensable for diagnosing hepatitis C virus (HCV) infection. Detection kits for anti-HCV antibodies (anti-HCV) are used for screening, and quantification kits for HCV RNA and HCV antigen (Ag) are used for the definite diagnosis of HCV infection or the evaluation of the pathological condition of and therapeutic effects in patients with chronic hepatitis C. Several kits are currently available for these purposes and are provided for clinical use in Japan. In this study, we aimed to evaluate the performance of these kits.MethodsWe used International Standards for HCV RNA and HCV Ag and a regional reference panel to evaluate the performance of thirteen anti-HCV, five HCV RNA, and two HCV Ag kits.ResultsAll specimens in the regional reference panel were diagnosed correctly by all anti-HCV kits, although the distributions of the quantified values varied, and the ratios of titer classification were not identical across kits. All HCV RNA kits quantified the International Standard with minimum deviation and diagnosed the specimens of the reference panel correctly. The quantified values of the International Standard by two HCV Ag kits were inconsistent. HCV Ag titers of some specimens were underestimated owing to the amino acid polymorphisms in comparison with HCV RNA titers.ConclusionsThe evaluation with International Standards and the regional reference panel was useful for assessing the quality of screening and diagnostic kits for HCV infection, and such quality control is essential for the clinical usage of these kits.

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Evaluation of dried blood spot testing using the Abbott Alinity i

Cited by 2 publications

References 22 publications

The role of dried blood spot tests in the detection of hepatitis B infection: A systematic review

The role of dried blood spot tests in the detection of hepatitis B infection: A systematic review

Performance Evaluation of In Vitro Screening and Diagnostic Kits for Hepatitis C Virus Infection

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