Evaluation of DNA/RNAshells for Room Temperature Nucleic Acids Storage

Liu, Xiaopan; Li, Qiyuan; Wang, Xian; Zhou, Xiaolin; He, Xuheng; Liao, Qiuyan; Zhu, Fengqin; Liu, Cheng; Zhang, Yong

doi:10.1089/bio.2014.0060

Cited by 17 publications

(10 citation statements)

References 15 publications

(17 reference statements)

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“…Preservation of RNA has been widely reviewed; many methods are available, either preservation of the clinical specimen itself or pure extracted RNA, the gold standard method for preservation of RNA is freezing at −80 °C ( Daum et al, 2011 ; Foss et al, 2016 ; Liu et al, 2015 ; Mathay et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature

Abdallah

Zaki

Abdel-Salam

2020

Diagnostic Microbiology and Infectious Disease

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Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus causing a highly fatal respiratory disease in humans. Confirmation of MERS-CoV infection and molecular study on the virus may require transportation of samples to specialized laboratories. While freezing at −80 °C is the gold standard method for RNA preservation, maintaining the integrity of viral RNA during transport will require additional precautions and, as a result, increase transport costs. We aimed at testing the stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature for 16 weeks. Respiratory samples spiked with stock culture of MERS-CoV were extracted and loaded on QIAamp Viral RNA Mini Kit spin columns and preserved at room temperature. Amount of viral RNA was evaluated periodically by real-time quantitative reverse-transcription polymerase chain reaction. Minimal changes in cycle threshold values over the study period were noted, suggesting stability of viral RNA by this preservation method.

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Section: Discussionmentioning

confidence: 99%

Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature

Abdallah

Zaki

Abdel-Salam

2020

Diagnostic Microbiology and Infectious Disease

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“…Описаны методы хранения ДНК в сухом состоянии: на коммерческих пластинах Biomatrica DNAstable, в 96-луночных планшетах c добавлением стабилизаторов ДНК (трегалозы или поливинилового спирта) [22], и в герметичных миникапсулах DNAshell из нержавеющей стали [23]. Все вышеописанные методы позволяют сохранять нуклеиновые кислоты при температуре окружающей среды в течение длительного времени, а качество подходит для последующих молекулярно-генетических тестов.…”

Section: Discussionunclassified

Shelf life of whole blood samples in a biobank and the yield of deoxyribonucleic acid during genetic testing

Скирко¹,

Мешков²,

Ефимова³

et al. 2020

Cardiovasc Ther Prev

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Aim. To study the effect of the shelf life of frozen whole blood samples in a biobank on the amount of released deoxyribonucleic acid (DNA).Material and methods. The study included whole blood samples placed in tubes with the anticoagulant EDTA (ethylenediaminetetraacetic acid at a concentration of 1,8 mg/ml) from participants in the epidemiological study ESSE-RF-1 and ESSE-RF-2 and cohort studies conducted at the National Medical Research Center for Therapy and Preventive Medicine. The samples were stored in the biobank of the National Medical Research Center for Therapy and Preventive Medicine at temperature from -22О C to -32О C. The shelf life from blood collection to DNA extraction ranged from several weeks to 11 years. DNA was extracted using QIAamp DNA Blood Mini Kit (250) and 96 Blood Kit (Qiagen, Germany). Statistical analysis was performed using the R 3.6.1 software. To analyze the association of blood storage time with the logarithm of DNA concentration, a linear regression was used.Results. The analysis included data on the DNA concentration of 5405 samples. Multivariate regression showed that the blood shelf life was significantly associated with a decrease in concentration by 3,92% (3,16-4,68) for each year of storage (p <0,0001). For 509 samples, the DNA concentration was measured twice, immediately after isolation and after 4,5 years of DNA storage at -32О C. During storage, the concentration of DNA increased by an average of 2% (p=0,046).Conclusion. Long-term storage of whole blood samples at temperature from -22О C to -32О C is associated with a decrease in the DNA yield. Long-term storage of the isolated DNA at a temperature of -32О C is not associated with a decrease in its concentration.

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“…The mid- and long-term stability of the quality of the samples conserved at low temperature is often difficult to maintain and control, requiring substantial precautions [ 52 , 53 , 54 ]. It is for this reason that several procedures have been developed to maintain the quality and stability at RT of nucleic acids over a long period of time for subsequent use in research [ 55 , 56 , 57 , 58 , 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

Washetine

Kara-Borni

Heeke

et al. 2018

Cancers

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Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact.

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Evaluation of DNA/RNAshells for Room Temperature Nucleic Acids Storage

Cited by 17 publications

References 15 publications

Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature

Stability of MERS-CoV RNA on spin columns of RNA extraction kit at room temperature

Shelf life of whole blood samples in a biobank and the yield of deoxyribonucleic acid during genetic testing

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA

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