Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction

Matuo, Youichirou; Izumi, Yoshinobu; Satô, Norihito; Yamamoto, Tatsuya; Shimizu, Kikuo

doi:10.1016/j.radmeas.2013.01.016

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…[11][12][13]. These lesions can prevent strand uncoiling, alter primer binding sites, and/or block DNA polymerase during PCR [14,15]. This results in allelic dropout, particularly for longer amplicons that incur DNA damage lesions with greater frequency [16], following sufficiently high doses (> 10 kGy) of γ-radiation [17,18].…”

Section: Foot Note Informationmentioning

confidence: 99%

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Goodwin

Wotherspoon

Gahan

et al. 2020

Forensic Sci Med Pathol

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Section: Foot Note Informationmentioning

confidence: 99%

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Goodwin

Wotherspoon

Gahan

et al. 2020

Forensic Sci Med Pathol

View full text Add to dashboard Cite

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“…However, the adverse effects of ROS can be prevented by supplementing with a diet rich in foods that contain antioxidants (legumes, fresh vegetables, and fruits, etc.). (4,5) Natural antioxidants such as polyphenol compounds have the ability to reduce free radicals, prevent oxidation effectively and are often found in natural products such as green tea or sour fruits. AA is well known as a protective agent.…”

Section: Introductionmentioning

confidence: 99%

Comparison of radiation protection effects between epigallocatechin gallate and ascorbic acid

Nhan,

Matuo,

Izumi

et al. 2023

Salud, Ciencia y Tecnología

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Ionizing radiation can originate from naturally occurring radiation sources on the earth or it can be from man-made sources. When interacting with cells and living organisms, ionizing radiation produces free radicals, impacting biological molecules such as proteins, lipids, and DNA in the cell nucleus and membrane, leading to cell death or causing cell mutations. Epigallocathecin gallate (EGCG) and ascorbic acid (AA) are well-known natural antioxidants that have been studied and applied as potential radical scavengers. In this study, the radiation protection effects in the presence of EGCG and AA via the scavenging process of free radicals (mainly hydroxyl radicals) were examined. Saccharomyces cerevisiae yeast cells were grown in YDP liquid medium containing yeast extract, peptone, and dextrose/glucose that supplemented with EGCG and AA at different concentrations. Then, the cell cultures were irradiated with both low (gamma) and high (helium ion beam) linear energy transfer (LET) radiations to evaluate the radiation effect on the survival of the yeast cell. Both of EGCG and AA play effectively important roles as radiation-protective agent for yeast cells and the effectiveness in radiation protection of EGCG and AA at the same concentration was almost the same between these two additives.

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Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer

Wada

Koike

Yamazaki

et al. 2021

Fusion Engineering and Design

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Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction

Cited by 4 publications

References 7 publications

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping

Comparison of radiation protection effects between epigallocatechin gallate and ascorbic acid

Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer

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