Evaluation of DNA Isolation and Amplification from Various Organs Preserved through Frozen, Formalin-Fixed and Paraffin-Embedded Tissue Sample method

Abstract

The purpose of this study was to compare the purity, concentration, and DNA band visualization of the isolated sample and PCR amplicon from three sample storage methods i.e. fresh frozen sample ( -20oC to -196oC), preserved in formalin, and paraffin wax. For this tissue samples were collected from the sample stored at frozen temperature -20oC, 10% NS formalin, and paraffin-embedded preparations, and Abs260/230 and Abs260/280 values and electrophoresis of 0.8% and 2% agarose gel visualization were analyzed. The results of the study showed a significant value of Abs260/280 for the isolated and amplified DNA purity. Among the tested three methods, frozen sample isolates and the PCR amplicon visualized a good DNA band. Meanwhile, the formalin-fixed and paraffinized tissue storage method showed a slightly lower quality DNA and no DNA band, respectively, while the PCR amplicon visualized a thin DNA band. In conclusion, all the tissue storage methods can be applied for DNA preservation and isolation, and the samples are successfully amplified on PCR examination.