Evaluation of DNA Damage Using 3 Comet Assay Parameters in Workers Occupationally Exposed to Lead

Kayaaltı, Zeliha; Yavuz, İlknur; Bacaksız, Ayşegül; Tutkun, Engın; Sayal, Ahmet; Söylemezoğlu, Tülın

doi:10.1080/19338244.2013.787964

Cited by 17 publications

(15 citation statements)

References 36 publications

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“…These findings were consistent with previous studies using workers who exposed to arsenic (23,24).This study is also compatible with several studies that arsenic causes genotoxic effects, chromosomal abnormality, sister chromatid exchange, DNA strand breakage, oxidative DNA damage (7,23,27). Studies carried on individuals who have been exposed to various chemicals and toxicants at different occupational settings have shown that DNA damage level increases along with occupational exposure (16,(32)(33)(34)(35)(36). In contrary to these studies, we could not find a statistically significant difference between exposure time and DNA damage.…”

Section: Discussionsupporting

confidence: 82%

Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Kayaaltı¹,

Kaya-Akyüzlü²,

Tutkun³

et al. 2017

Marmara Pharmaceutical Journal

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The purpose of this study is to investigate the association between DNA damage and blood arsenic levels in individuals occupationally exposed to arsenic (As). A hundred-twenty exposed individuals from silver mining plate workers were monitored in the way of DNA damage in blood lymphocytes using comet assay (single cell gel electrophoresis, SSGE). The DNA damage levels were measured by BAB Bs Comet Assay system and As levels were analyzed by the atomic absorption spectrometer (AAS) system. The mean blood arsenic level was 17.03±10.85 µg/L. A statistically significant positive correlations were found between the arsenic levels and comet parameters (tail intensity, comet length and tail length) (r=0.360, r=0.334, r=0.259, respectively, p<0.01). Our results showed that exposure to arsenic may cause DNA damage in peripheral lymphocytes of Turkish silver mining plate workers.

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Section: Discussionsupporting

confidence: 82%

Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Kayaaltı¹,

Kaya-Akyüzlü²,

Tutkun³

et al. 2017

Marmara Pharmaceutical Journal

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“…Due to significant toxicity associated with Pb, CDC[ 5 ] has defined the standard BLL to be <10 μg/dl in adults and also has emphasized on biomonitoring in occupationally exposed workers. [ 3 5 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 1 2 ] However, it has been associated with several adverse health effects and, hence, currently has become a global health concern. [ 3 ]…”

Section: Introductionmentioning

confidence: 99%

Blood lead levels among the occupationally exposed workers and its effect on calcium and vitamin D metabolism: A case-control study

Batra

Thakur

Meena³

et al. 2020

J Family Med Prim Care

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Introduction: Lead (Pb) is one of the major occupational pollutants present in the developed and developing countries including India. In humans, Pb can cause a wide range of biological effects depending upon the level and duration of exposure. The goal of this study was to evaluate the blood lead levels (BLLs) and its associated effects on vitamin D and calcium metabolism, among the workers occupationally exposed to Pb. Materials and Methods: This cross-sectional, case-control study was conducted for a period of 18 months (January 2017 to July 2018). A total of 160 subjects were included in the study (80 in each, Pb-exposed group and control group). The blood Pb levels were quantified by using an inductively coupled plasma mass spectrometry with triple quadrupole technology (iCAP™ TQ ICP-MS). Other biochemical parameters were estimated using fully automatic analyzer by RANDOX, RX-imola, Crumlin, UK and Johnson and Johnson, VITROS® ECiQ, Immunodiagnostic system, Ortho Clinical Diagnostics, New Jersey, USA. Results: Upon analysis it was observed that serum calcium, phosphorous, and vitamin D levels were significantly decreased (8.35 ± 0.42 mg/dl, 3.07 ± 0.34 mg/dl, and 28.82 ± 10.81 ng/ml respectively; P < 0.001), whereas the BLL and serum iPTH levels were significantly increased (38.02 ± 19.92 μg/dl and 116.78 ± 19.93 pg/ml respectively; P < 0.001) in Pb exposed subjects as compared to control subjects. Conclusion: Our study results demonstrated that high BLL significantly alter vitamin D and calcium metabolism. The data extrapolated from our study emphasizes the necessity of surveillance in exposed workers. As the associated deleterious effects of Pb-exposure can be serious, we propose that a routine-periodical screening of the workers exposed to lead should be conducted.

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“…SCGE is a technique which is low cost and sensitive; however, the published results related to smoking have, sometimes, not confirmed the damage in a convincing manner. Some authors [20, 21] state that the lack of agreement upon the methodology is the main cause of variations in the results; in addition, most use only around 3 to 5 parameters when scoring genome instability, this is in contrast to our use of 16 variables added to the PCA procedure.…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, some studies have failed to find a significant difference between smokers and non-smokers using SCGE or significant association dependent on the score methods [12, 24]. On the other hand, some others have concluded that smoking can cause DNA instability when analyzed on peripheral blood cells as measured by the comet assay [21, 25]. We actually found a significant correlation with the smoking index, which has indeed been reported to have an involvement in the development of some diseases such as chronic obstructive pulmonary disease and lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Smoking index, lifestyle factors, and genomic instability assessed by single-cell gel electrophoresis: a cross-sectional study in subjects from Yucatan, Mexico

et al. 2019

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BackgroundIt is widely accepted that genomic instability is associated with several mechanisms involving oxidative stress, which can increase the rate of DNA breaks. Such factors include smoking, impairments in body composition, an unhealthy lifestyle, and a hereditary history of cancer. The aim was to evaluate the degree of association of genomic instability in smokers and non-smokers, and how the risk could change depending on the lifestyle and other causes. For this purpose, a survey of tobacco consumption, dietary patterns, physical activity, antecedents of cancer, and body composition assessment was carried out. Genomic instability was evaluated through a single-cell gel electrophoresis using peripheral blood mononuclear cells in three different conditions of oxidative stress. The analysis of genomic damage degree was performed through a dimension reduction procedure (principal component analysis) from 16 parameters per treatment (adding up 48 parameters of genomic damage per subject) and a binary logistic regression model for DNA fragmentation risk.ResultsThe sample consisted of 82 participants, divided into three age groups: young adults (18–35 years), adults (36–59 years), and older adults (60–95 years). As expected, the results showed a significant positive correlation of age with genomic damage rates, represented by 2 PCA groups (p = 0.027, p = 0.004). There were consistent significant positive associations of genomic damage rates with smoking index and three PCA groups (p = 0.007, p = 0.004, p = 0.009). The smoking status and age group analysis revealed that there were significant differences for adult smokers with the same aforementioned PCA groups (p = 0.002, p = 0.001, p = 0.010). In addition, higher DNA damage rates were found in subjects with incorrect diet patterns, long sitting hours, and previous exposure to radiation. The analysis with binary logistic regression displayed two models in which lifestyles (age, diet, and/or sedentarism) did not change the significance of smoking index for DNA fragmentation risk; however, when physical activity was present in the model, the smoking index was not a significant factor for DNA damage risk.ConclusionsAlthough it is well known that smoking affects human health in different ways, DNA fragmentation can be analyzed by a damage phenotypic analysis and integrate a risk analysis reshaped by diet and lifestyle in general.

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Evaluation of DNA Damage Using 3 Comet Assay Parameters in Workers Occupationally Exposed to Lead

Cited by 17 publications

References 36 publications

Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Evaluation of Relationship Between Lymphocyte DNA Damages and Blood Arsenic Levels in Silver Mining Workers using Alkaline Comet Assay

Blood lead levels among the occupationally exposed workers and its effect on calcium and vitamin D metabolism: A case-control study

Smoking index, lifestyle factors, and genomic instability assessed by single-cell gel electrophoresis: a cross-sectional study in subjects from Yucatan, Mexico

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