Evaluation of DNA barcoding to facilitate the authentication of processed fish products in the seafood industry

Adibah, A.B.; Syazwan, S.; Hanim, M.Z. Haniza; Munir, Misbakhul; Faraha, A.G. Intan; Azizah, Mohd Nor Siti

doi:10.1016/j.lwt.2020.109585

Cited by 17 publications

(6 citation statements)

References 33 publications

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“…On the other hand, the control band of the 16S rDNA gene amplified fragments at concentrations of as low as 0.01 ng/µL for the Control Region, and 1 ng/µL for the COI. Frozen and highly processed foods are more susceptible to the fragmentation and low concentrations of DNA, which can affect their amplification by PCR ( Böhme et al, 2019 ; Adibah et al, 2020 ). The sensitivity tests were run on the DNA of frozen fish, which may account for the differences in the detection limits.…”

Section: Discussionmentioning

confidence: 99%

Development of rapid and cost-effective multiplex PCR assays to differentiate catfish of the genus Brachyplatystoma (Pimelodidae–Siluriformes) sold in Brazil

Freitas

Barbosa

Vale

et al. 2023

PeerJ

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The catfishes Brachyplatystoma filamentosum (Kumakuma), Brachyplatystoma vaillantii (Laulao catfish), and Brachyplatystoma rousseauxii (gilded catfish) are important fishery resources in Brazil, where they are sold both fresh and in the form of fillets or steaks. These species have morphological similarities, thus, they can be easily misidentified or substituted, especially after processed. Therefore, accurate, sensitive, and reliable methods are needed for the identification of these species to avoid commercial fraud. In the present study, we develop two multiplex PCR assays for the identification of the three catfish species. Each multiplex protocol combined three species-specific forward primers and a universal reverse primer to produce banding patterns able to discriminate the target species unequivocally. The length of the cytochrome C oxidase subunit I (COI) fragments was approximately 254 bp for B. rousseauxii, 405 bp for B. vaillantii, and 466 bp for B. filamentosum, while the control region (CR) assay produced fragments of approximately 290 bp for B. filamentosum, 451 bp for B. vaillantii, and 580 bp for B. rousseauxii. The protocols were sensitive enough to detect the target species at a DNA concentration of 1 ng/µL, with the exception of the CR of B. vaillantii, in which the fragment was only detectable at 10 ng/µL. Therefore, the multiplex assays developed in the present study were sensitive, accurate, efficient, rapid, and cost-effective for the unequivocal identification of the target species of Brachyplatystoma. They can be utilized by fish processing industries to certify their products, or by government agencies to authenticate products and prevent fraudulent commercial substitutions.

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Section: Discussionmentioning

confidence: 99%

Development of rapid and cost-effective multiplex PCR assays to differentiate catfish of the genus Brachyplatystoma (Pimelodidae–Siluriformes) sold in Brazil

Freitas

Barbosa

Vale

et al. 2023

PeerJ

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“…Para detectar adulteración mediante la adición de azúcar derivada de las plantas tipo C4 en jugos de fruta se utiliza la técnica IRMS (método AOAC 2004.01), y la técnica SNIF-NMR para la detección de chaptalización (técnica especial de azucarado) mediante la adición de azúcar derivada de la remolacha (método AOAC 995.17) 17 . En pescados y productos del mar, las técnicas analíticas disponibles permiten detectar autenticación, trazabilidad, sustitución o mezcla, identificación, adulteración y etiquetado incorrecto 18,19,20,21,22,23 . En carnes y productos cárnicos, se puede detectar el tipo de especie animal, adulteración, autenticación y etiquetado incorrecto 24,25,26,27,28 .…”

Section: Herramientas Analíticas Para La Detecciónunclassified

Fraude alimentario: Pasado, presente y futuro

Escobar

Beas²,

Gras³

et al. 2023

Rev. chil. nutr.

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“…Unintentional substitution may occur due to morphological similarities between closely related species or uncertainties in the common names used for species marketed domestically or internationally [6]. In contrast, intentional substitution is typically performed to increase profitability and may involve the sale of lower-value or endangered species [7]. Therefore, there is a need for an authentication method that can distinguish between different laver species to prevent mislabeling and provide accurate information to consumers.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Simultaneous Authentication of Six Laver Species Using Capillary Electrophoresis-Based Multiplex PCR

Yang,

Kim,

Kim

et al. 2024

Foods

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Lavers are typically consumed in dried or seasoned forms. However, commercially processed lavers can lead to seafood fraud because it is impossible to authenticate the original species based on morphological characteristics alone. In this study, we developed a capillary electrophoresis-based multiplex polymerase chain reaction (PCR) to authenticate six different laver species. The species-specific primer sets to target the chloroplast rbcL or rbcS genes were newly designed. We successfully established both singleplex and multiplex conditions, which resulted in specific amplicons for each species (N. dentata, 274 bp; N. yezoensis, 211 bp; N. seriata, 195 bp; N. tenera, 169 bp; N. haitanensis, 127 bp; P. suborbiculata, 117 bp). Moreover, the assays were sensitive enough to detect DNA ranging from 10 to 0.1 pg of DNA. The optimized capillary electrophoresis-based multiplex PCR was successfully applied to 40 commercial laver products. In addition to detecting the laver species as stated on the commercial label, the assay discovered cases where less expensive species were mixed in. With its advantageous properties, such as short amplicon size, high specificity, and superior sensitivity, this assay could be used for the authentication of the six laver species.

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Evaluation of DNA barcoding to facilitate the authentication of processed fish products in the seafood industry

Cited by 17 publications

References 33 publications

Development of rapid and cost-effective multiplex PCR assays to differentiate catfish of the genus Brachyplatystoma (Pimelodidae–Siluriformes) sold in Brazil

Development of rapid and cost-effective multiplex PCR assays to differentiate catfish of the genus Brachyplatystoma (Pimelodidae–Siluriformes) sold in Brazil

Fraude alimentario: Pasado, presente y futuro

Rapid and Simultaneous Authentication of Six Laver Species Using Capillary Electrophoresis-Based Multiplex PCR

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