Evaluation of diversity and conservation status of Matricaria chamomilla (L.) and Matricaria aurea (Loefl.) Sch. Bip. in Lebanon

Soubra, Noura; Yazbek, Mariana; Noun, J.; Talhouk, Rabih; Tanios, Sabine; Karam, N.

doi:10.4172/2332-2543.1000206

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…За посівними площами вона займає першість в Європі серед інших лікарських рослин. Про цілющі властивості рослини відомо здавна, коли вона ще використовувалася для лікування в Стародавньому Єгипті, Греції та Римі (Soubra et al, 2018). Понад 120 хімічних сполук синтезуються рослиною як вторинні метаболіти, що обумовлюють певний терапевтичний ефект (Singh, Khanam, Mirsa, & Srivastava, 2011;Aksoy & Sӧzbilir, 2012).…”

Section: вступunclassified

Ecological aspect of growing Matricaria recutita L. plants in Western Ukraine

Lupak¹,

Antonyak²

2020

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The influence of soil and climatic conditions and growth biostimulants on the content of biochemical indices of prooxidant and antioxidant processes in the inflorescences of M. recutita plants of cv. "Perlyna Lisostepu" was investigated. The plants were grown on sod-podzolic soil of Precarpathian and dark-gray podzolic medium loamy soil of the Western Forest-Steppe of Ukraine under the influence of growth biostimulants "Vermymag", "Vermyiodis" and "Vermystym". The content of enzymes and low molecular weight metabolites in inflorescence cells was determined spectrophotometrically. Superoxide dismutase was determined by the ability of the enzyme to inhibit the photochemical reduction of nitroblue tetrazolium. Peroxidase activity was analyzed by the oxidation of aminoantipyrine, and catalase activity was analyzed by the ability of hydrogen peroxide to form a stable colored compound with ammonium molybdate. The content of ascorbic acid was determined by method of Murrie with Tillmann's reagent. Analysis of TBA (Thiobarbituric Acid) -active products was performed by determining the content of malonic dialdehyde in the interaction of MDA with 2-thiobarbituric acid. The content of essential oil in the inflorescences of M. recutita was determined by steam distillation method. An increase (p < 0.05) in the activity of enzymes of the antioxidant system in the inflorescences of M. recutita plants, grown with the application of biostimulants "Vermymag" (by 14.9-22.9%) and "Vermyiodis" (by 16.1-23.4%) in comparison with the control was established. Vermystym had no significant influence on enzyme activity. Inflorescences of plants of all experimental variants were characterized by a significantly higher content of ascorbic acid compared to plants grown in the control. The application of biostimulants promoted to the suppression of lipoperoxidation processes in plant cells. A comparative analysis of biochemical indices of inflorescences of M. recutita cultivated species with wild species was made. In the inflorescences of wild plants M. recutita was found a higher content of catalase relative to plants of cultivated species. The results of the investigation of the essential oil content in the inflorescences of M. recutita plants cv. "Perlyna of Lisostepu" showed that the medicinal plant raw materials had the high quality. The results of the investigation of the content of essential oil in the inflorescences of M. recutita plants of cv. "Perlyna Lisostepu" indicated that the medicinal plant raw materials were of high quality. However, the content of essential oil in the inflorescences of the wild species M. recutita does not meet the requirements of the State Pharmacopoeia of Ukraine. By means of research it was established that soil and climatic conditions did not influence the activity of enzymes, lipoperoxidation processes and the content of essential oil. However, plants grown in the conditions of Western Forest-Steppe were characterized by the significantly higher content of ascorbic acid.

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Section: вступunclassified

Ecological aspect of growing Matricaria recutita L. plants in Western Ukraine

Lupak¹,

Antonyak²

2020

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“…Since ancient times, the Egyptians, Greeks, Romans, and Arabs have used chamomile, one of the most popular herbs in the world. Matricaria aurea , also known as golden chamomile ( Soubra et al, 2018 ), is reported to be used in traditional medicine in some Middle Eastern countries as an antidiabetic herb ( Yaniv et al, 1987 ; Al-Mustafa and Al-Thuniba, 2008 ). It is also widely used as a therapy for a variety of other conditions, including inflammation, respiratory disorders, facilitating delivery during pregnancy, and infant health care ( Ali-Shtayeh et al, 2015 ; Abdelhalim et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Integrating experimental model, LC-MS/MS chemical analysis, and systems biology approach to investigate the possible antidiabetic effect and mechanisms of Matricaria aurea (Golden Chamomile) in type 2 diabetes mellitus

et al. 2022

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Type 2 diabetes mellitus (T2DM) is a heterogeneous disease with numerous abnormal targets and pathways involved in insulin resistance, low-grade inflammation, oxidative stress, beta cell dysfunction, and epigenetic factors. Botanical drugs provide a large chemical space that can modify various targets simultaneously. Matricaria aurea (MA, golden chamomile) is a widely used herb in Middle Eastern communities for many ailments, including diabetes mellitus, without any scientific basis to support this tradition. For the first time, this study aimed to investigate the possible antidiabetic activity of MA in a type 2 diabetic rat model, identify chemical constituents by LC-MS/MS, and then elucidate the molecular mechanism(s) using enzyme activity assays, q-RTPCR gene expression analysis, network pharmacology analysis, and molecular docking simulation. Our results demonstrated that only the polar hydroethanolic extract of MA had remarkable antidiabetic activity. Furthermore, it improved dyslipidemia, insulin resistance status, ALT, and AST levels. LC-MS/MS analysis of MA hydroethanolic extract identified 62 compounds, including the popular chamomile flavonoids apigenin and luteolin, other flavonoids and their glycosides, coumarin derivatives, and phenolic acids. Based on pharmacokinetic screening and literature, 46 compounds were chosen for subsequent network analysis, which linked to 364 candidate T2DM targets from various databases and literature. The network analysis identified 123 hub proteins, including insulin signaling and metabolic proteins: IRS1, IRS2, PIK3R1, AKT1, AKT2, MAPK1, MAPK3, and PCK1, inflammatory proteins: TNF and IL1B, antioxidant enzymes: CAT and SOD, and others. Subsequent filtering identified 40 crucial core targets (major hubs) of MA in T2DM treatment. Functional enrichment analyses of the candidate targets revealed that MA targets were mainly involved in the inflammatory module, energy-sensing/endocrine/metabolic module, and oxidative stress module. q-RTPCR gene expression analysis showed that MA hydroethanolic extract was able to significantly upregulate PIK3R1 and downregulate IL1B, PCK1, and MIR29A. Moreover, the activity of the antioxidant hub enzymes was substantially increased. Molecular docking scores were also consistent with the networks’ predictions. Based on experimental and computational analysis, this study revealed for the first time that MA exerted antidiabetic action via simultaneous modulation of multiple targets and pathways, including inflammatory pathways, energy-sensing/endocrine/metabolic pathways, and oxidative stress pathways.

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“…Broad sense heritability and genetic advance were important for plant breeding programme (Herbert et al 1955). In addition to the phenotypic traits, the mutagenic effects can be accurately assessed using DNA molecular marker techniques used to identify and evaluate genetic diversity between plant species, genotypes and cultivars (El-Nashar and Ammar 2016; Soubra et al, 2018). The goal of the present study was to assess the efficiency of different concentrations of colchicine in induction of new agronomic, chemical and yield components of Calendula mutant plants.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

Yassein¹

2021

J microb biotech food sci

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Calendula officinalis L. (pot marigold) is one of the main aromatic and medicinal plants with many uses in food and medicines. This study was carried out to demonstrate the efficiency of six colchicine concentrations (0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 per cent, w/v) for Calendula improvement and induction of genetic variation. Colchicine treatments had a positive effect on the number of branches/plant, number of inflorescences, fresh and dry weight of inflorescences, inflorescence diameter, total soluble carbohydrates and β-carotene except for plant height, while seed germination and plant height were reduced. Estimation of heritability, genetic advance, genetic variability and selection of superior genotypes will be an important object in crop breeding and genetic improvement programs, and selection of genotypes with higher desirable characters. Heritability was high and ranged from 48.64 to 90.81, respectively (inflorescence diameter and plant height, respectively). Molecular markers based on a RAPD-PCR study elucidate the classification of induced Calendula mutants into two clusters. The coefficient of genetic diversity was estimated at 30%. A combination of morphological and physiological responses with molecular data contained in the various colchicine treatments illustrated the utility of RAPD-PCR as a method for identifying useful mutants and could be used to detect the colchicine effect significantly. Findings recommend the 0.05 per cent colchicine for efficient breeding calendula mutation and genetic improvement.

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Evaluation of diversity and conservation status of Matricaria chamomilla (L.) and Matricaria aurea (Loefl.) Sch. Bip. in Lebanon

Cited by 4 publications

References 20 publications

Ecological aspect of growing Matricaria recutita L. plants in Western Ukraine

Ecological aspect of growing Matricaria recutita L. plants in Western Ukraine

Integrating experimental model, LC-MS/MS chemical analysis, and systems biology approach to investigate the possible antidiabetic effect and mechanisms of Matricaria aurea (Golden Chamomile) in type 2 diabetes mellitus

Morphological and Genetic Diversity Analysis in Calendula (Calendula Officinalis L.) Influenced by Mutagenic Effect of Colchicine

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