Evaluation of disulfide bond-conjugated LMWSC-g-bPEI as non-viral vector for low cytotoxicity and efficient gene delivery

Jeong, Gyu-Seob; Nah, Jae‐Woon

doi:10.1016/j.carbpol.2017.09.048

Cited by 25 publications

(14 citation statements)

References 39 publications

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“…Under similar conditions, CS-BPEI copolymers were produced as non-viral gene carriers targeting chondrocytes and synoviocytes [ 59 ]. The conjugation of CS with BPEI-cystamine, in the presence of CDI, led to the formation of bioreducible copolymers for gene delivery [ 60 ]. While 1 H NMR analysis of the final system gave evidence for the combination of CS and BPEI, one cannot disregard that the copolymers were assembled by non-covalent interactions rather than bis-urea bonds due to the fast degradation of CDI in aqueous medium (see Section 5 for more details on the characterization of covalent CS-based copolymers).…”

Section: Strategies For Covalent Functionalizationmentioning

confidence: 99%

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

2021

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Chitosan (CS) is a natural biopolymer that has gained great interest in many research fields due to its promising biocompatibility, biodegradability, and favorable mechanical properties. The versatility of this low-cost polymer allows for a variety of chemical modifications via covalent conjugation and non-covalent interactions, which are designed to further improve the properties of interest. This review aims at presenting the broad range of functionalization strategies reported over the last five years to reflect the state-of-the art of CS derivatization. We start by describing covalent modifications performed on the CS backbone, followed by non-covalent CS modifications involving small molecules, proteins, and metal adjuvants. An overview of CS-based systems involving both covalent and electrostatic modification patterns is then presented. Finally, a special focus will be given on the characterization techniques commonly used to qualify the composition and physical properties of CS derivatives.

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Section: Strategies For Covalent Functionalizationmentioning

confidence: 99%

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

2021

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“…Furthermore, it is practically easy to create and chemically alter non-viral vectors on a large scale. Most importantly, the non-viral vector system is not restricted by the molecular size of the gene to be introduced [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

The Development of Functional Non-Viral Vectors for Gene Delivery

Patil

Gao

Lin

et al. 2019

IJMS

194

135

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Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, in general, should be mediated by gene vectors. Non-viral vectors, as promising delivery systems, have received considerable attention due to their low cytotoxicity and non-immunogenicity. As research continued, more and more functional non-viral vectors have emerged. They not only have the ability to deliver a gene into the cells but also have other functions, such as the performance of fluorescence imaging, which aids in monitoring their progress, targeted delivery, and biodegradation. Recently, many reviews related to non-viral vectors, such as polymers and cationic lipids, have been reported. However, there are few reviews regarding functional non-viral vectors. This review summarizes the common functional non-viral vectors developed in the last ten years and their potential applications in the future. The transfection efficiency and the transport mechanism of these materials were also discussed in detail. We hope that this review can help researchers design more new high-efficiency and low-toxicity multifunctional non-viral vectors, and further accelerate the progress of gene therapy.

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“…Consequently, more and more scientists have turned their attention to the development of non-viral vectors and carriers [ 29 , 30 , 31 ]. Recent studies have shown that non-viral vectors have the following advantages: low immunogenicity, biodegradability, easy synthesis, low cost of production, and no restriction on the size of the molecules to be introduced [ 32 , 33 , 34 , 35 , 36 ]. The most extensively researched non-viral vectors are mainly polymers, liposomes, and nanoparticles [ 37 , 38 , 39 , 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Non-Viral Vectors in Drug Delivery and Gene Therapy

Ren

Wang

et al. 2021

Polymers

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Vectors and carriers play an indispensable role in gene therapy and drug delivery. Non-viral vectors are widely developed and applied in clinical practice due to their low immunogenicity, good biocompatibility, easy synthesis and modification, and low cost of production. This review summarized a variety of non-viral vectors and carriers including polymers, liposomes, gold nanoparticles, mesoporous silica nanoparticles and carbon nanotubes from the aspects of physicochemical characteristics, synthesis methods, functional modifications, and research applications. Notably, non-viral vectors can enhance the absorption of cargos, prolong the circulation time, improve therapeutic effects, and provide targeted delivery. Additional studies focused on recent innovation of novel synthesis techniques for vector materials. We also elaborated on the problems and future research directions in the development of non-viral vectors, which provided a theoretical basis for their broad applications.

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Evaluation of disulfide bond-conjugated LMWSC-g-bPEI as non-viral vector for low cytotoxicity and efficient gene delivery

Cited by 25 publications

References 39 publications

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

The Development of Functional Non-Viral Vectors for Gene Delivery

Application of Non-Viral Vectors in Drug Delivery and Gene Therapy

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