Evaluation of dissolved and immobilized redox mediators on dark fermentation: Driving to hydrogen or solventogenic pathway

Atilano-Camino, Marina M.; Luévano-Montaño, Cindy D.; García-González, Alcione; Olivo-Alanís, Daniel; Álvarez, Luis H.; García-Reyes, Refugio Bernardo

doi:10.1016/j.biortech.2020.123981

Cited by 15 publications

(7 citation statements)

References 42 publications

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“…Recently, it has been shown that production of hydrogen or SCFAs and alcohols during dark fermentation can be controlled by redox mediators that change the redox potential and the electron transfer between enzymatic complexes and drive NADH toward reactions leading to hydrogen formation (Atilano-Camino et al, 2020). Furthermore, it was found that C. pasteurianum changed its metabolites from acetate, hydrogen, carbon dioxide and butyrate to lactate, ethanol and butanol under the conditions of low pH and iron and phosphate deficit (Dabrock et al, 1992).…”

Section: Product Inhibition In Hydrogen-producing Pbrsmentioning

confidence: 99%

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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This study describes the dynamics and complexity of microbial communities producing hydrogen-rich fermentation gas from sugar-beet molasses in five packed-bed reactors (PBRs). The bioreactors constitute a part of a system producing hydrogen from the by-products of the sugar-beet industry that has been operating continuously in one of the Polish sugar factories. PBRs with different working volumes, packing materials, construction and inocula were tested. This study focused on analysis (based on 16S rRNA profiling and shotgun metagenomics sequencing) of the microbial communities selected in the PBRs under the conditions of high (>100 cm3/g COD of molasses) and low (<50 cm3/g COD of molasses) efficiencies of hydrogen production. The stability and efficiency of the hydrogen production are determined by the composition of dark fermentation microbial communities. The most striking difference between the tested samples is the ratio of hydrogen producers to lactic acid bacteria. The highest efficiency of hydrogen production (130–160 cm3/g COD of molasses) was achieved at the ratios of HPB to LAB ≈ 4:2.5 or 2.5:1 as determined by 16S rRNA sequencing or shotgun metagenomics sequencing, respectively. The most abundant Clostridium species were C. pasteurianum and C. tyrobutyricum. A multiple predominance of LAB over HPB (3:1–4:1) or clostridia over LAB (5:1–60:1) results in decreased hydrogen production. Inhibition of hydrogen production was illustrated by overproduction of short chain fatty acids and ethanol. Furthermore, concentration of ethanol might be a relevant marker or factor promoting a metabolic shift in the DF bioreactors processing carbohydrates from hydrogen-yielding toward lactic acid fermentation or solventogenic pathways. The novelty of this study is identifying a community balance between hydrogen producers and lactic acid bacteria for stable hydrogen producing systems. The balance stems from long-term selection of hydrogen-producing microbial community, operating conditions such as bioreactor construction, packing material, hydraulic retention time and substrate concentration. This finding is confirmed by additional analysis of the proportions between HPB and LAB in dark fermentation bioreactors from other studies. The results contribute to the advance of knowledge in the area of relationships and nutritional interactions especially the cross-feeding of lactate between bacteria in dark fermentation microbial communities.

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Section: Product Inhibition In Hydrogen-producing Pbrsmentioning

confidence: 99%

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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“…Despite the importance of conducting materials in effectively facilitating electron transfer, it is imperative to note that these materials play a pivotal role in determining the fate of biobased product formation [ 88 ]. Though the biocatalyst and the microenvironment dictate metabolic pathways and the corresponding biobased products, the role of electron flux is crucial in the carbon chain length, titer, and switching toward specific products’ formation, viz., short/medium-chain carboxylic acids (acidogenesis/carboxylate platform), short/medium-chain alcohols (solventogenesis), and biopolymers/bioplastics (anoxygenesis) [ 32 , 89 , 90 , 91 ]. The carboxylate platform/acidogenesis is comparatively a more advantageous bioprocess than others in terms of generating lower CO 2 emissions compared to sugar platforms.…”

Section: Sustainable Interventionmentioning

confidence: 99%

Advances in Biological Wastewater Treatment Processes: Focus on Low-Carbon Energy and Resource Recovery in Biorefinery Context

Sravan,

Matsakas,

Sarkar

2024

Bioengineering

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Advancements in biological wastewater treatment with sustainable and circularity approaches have a wide scope of application. Biological wastewater treatment is widely used to remove/recover organic pollutants and nutrients from a diverse wastewater spectrum. However, conventional biological processes face challenges, such as low efficiency, high energy consumption, and the generation of excess sludge. To overcome these limitations, integrated strategies that combine biological treatment with other physical, chemical, or biological methods have been developed and applied in recent years. This review emphasizes the recent advances in integrated strategies for biological wastewater treatment, focusing on their mechanisms, benefits, challenges, and prospects. The review also discusses the potential applications of integrated strategies for diverse wastewater treatment towards green energy and resource recovery, along with low-carbon fuel production. Biological treatment methods, viz., bioremediation, electro-coagulation, electro-flocculation, electro-Fenton, advanced oxidation, electro-oxidation, bioelectrochemical systems, and photo-remediation, are summarized with respect to non-genetically modified metabolic reactions. Different conducting materials (CMs) play a significant role in mass/charge transfer metabolic processes and aid in enhancing fermentation rates. Carbon, metal, and nano-based CMs hybridization in different processes provide favorable conditions to the fermentative biocatalyst and trigger their activity towards overcoming the limitations of the conventional process. The emerging field of nanotechnology provides novel additional opportunities to surmount the constraints of conventional process for enhanced waste remediation and resource valorization. Holistically, integrated strategies are promising alternatives for improving the efficiency and effectiveness of biological wastewater treatment while also contributing to the circular economy and environmental protection.

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“…Catalysts 2021, 11, x FOR PEER REVIEW 12 of 32 optimal condition for hydrogen production, while 3.5% of salinity and pH = 5 are able to inhibit the production [112]. The use of lawsone and anthraquinone 2-sulphonate covalently immobilized on activated carbon, as redox mediators, has been evaluated in the dark fermentation of glucose by a pretreated anaerobic sludge [113]. The results showed that the use of lawsone increased the hydrogen production of 10%, while anthraquinone 2-sulphonate improved the hydrogen production rate of 11.4%.…”

Section: Dark-fermentationmentioning

confidence: 99%

“…For example, studies on the effect of shear velocity on hydrogen production, in a dynamic membrane bioreactor, containing a 50 μm polyester mesh as support material, have shown that the homoacetogenic pathway can minimized by choosing the optimal shear velocity, thus improving the hydrogen production [118]. The use of lawsone and anthraquinone 2-sulphonate covalently immobilized on activated carbon, as redox mediators, has been evaluated in the dark fermentation of glucose by a pretreated anaerobic sludge [113]. The results showed that the use of lawsone increased the hydrogen production of 10%, while anthraquinone 2-sulphonate improved the hydrogen production rate of 11.4%.…”

Section: Dark-fermentationmentioning

confidence: 99%

Main Hydrogen Production Processes: An Overview

et al. 2021

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Due to its characteristics, hydrogen is considered the energy carrier of the future. Its use as a fuel generates reduced pollution, as if burned it almost exclusively produces water vapor. Hydrogen can be produced from numerous sources, both of fossil and renewable origin, and with as many production processes, which can use renewable or non-renewable energy sources. To achieve carbon neutrality, the sources must necessarily be renewable, and the production processes themselves must use renewable energy sources. In this review article the main characteristics of the most used hydrogen production methods are summarized, mainly focusing on renewable feedstocks, furthermore a series of relevant articles published in the last year, are reviewed. The production methods are grouped according to the type of energy they use; and at the end of each section the strengths and limitations of the processes are highlighted. The conclusions compare the main characteristics of the production processes studied and contextualize their possible use.

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Evaluation of dissolved and immobilized redox mediators on dark fermentation: Driving to hydrogen or solventogenic pathway

Cited by 15 publications

References 42 publications

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Advances in Biological Wastewater Treatment Processes: Focus on Low-Carbon Energy and Resource Recovery in Biorefinery Context

Main Hydrogen Production Processes: An Overview

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