Evaluation of disinfectant “Enzidez” according to physical and chemical parameters

Abstract

The dependence of the national economy on disinfectants is constantly growing through prevention strategies and the development of resistance in microorganisms. Therefore, new disinfectants with different mechanisms of biocidal activity for a wide range of pathogens are appearing on the market. The aim of work has been to determine the physical and chemical parameters of the created disinfectant “Enzidez” active on biofilm forms of bacteria and the possible organic load. The magnitude of the surface tension of disinfectant “Enzidez” at different concentrations of solutions has been determined using a device with a Traube stalagmometer. The wettability of solutions has been determined by the method of measuring the wetting angle with a catheter. Washing properties and stability of foam by the method of washing animal fats, followed by application of indicator liquid. Proteolytic activity has been determined using milk and the effect of Enzidez solutions of different concentrations on it, followed by measurement of the optical density of the solutions spectrophotometrically at a wavelength of 600 nm. It is established that the disinfectant with enzymes – “Enzidez” for disinfection in veterinary clinics for pre-sterilization disinfection and treatment of products and instruments of surgical purpose (plastic, rubber, glass, stainless steel) and objects of veterinary supervision (tables, utensils, walls, floors, doors, windows, etc.) with organic load is well soluble in water and has a pH of 0.25–1.0 % solutions in the range of 8.2–8.0 units. At a solution temperature of +20 ± 1 °С it has had a surface tension not higher than 37.63 ± 0.50 mN/m and a wetting angle of 69.5 ± 0.7 degree. The disinfectant has provided an excellent detergent effect only at solutions with a concentration of 1.0 % and above. When assessing the corrosive action of Enzidez in the concentration of solutions from 0.25 to 1.0 %, the amount of corrosion on stainless steel has been hundreds of times lower than the allowable regulatory limit. The impact on galvanized steel is slightly higher than that of stainless steel, but also several tens below the norm. A study to determine the proteolytic activity of the disinfectant “Enzidez” on milk proteins has revealed that the disinfectant broke down proteins at a concentration of solutions 0.5–1.0 % at a temperature of + 20 °C at 41.3–43.1 %, respectively, for 15 min of exposure. However, increasing the temperature of solutions to +60 °C and exposure for 30 min has provided an increase in proteolytic activity up to 70 %. Thus, disinfectant “Enzidez” is a highly active disinfectant for disinfecting surfaces even in the case of organic contamination.

Evaluation of the disinfectant “Enzidez” according to indicators of acute toxicity and irritant effect

“…При цьому основні дослідження проводяться у лабораторних умовах, а тому застосовані методи мають відтворювати їхню ефективність у виробничих випробуваннях. Чинники, що мають особливе значення для встановлення біоцидного ефекту в лабораторних дослідженнях, включають: температуру, концентрацію дезінфікуючого засобу та час впливу, органічне середовище, біоплівку, якість поверхні та її змочуваність водою тощо (Kozlovska et al, 2017;Lineback et al, 2018;Wales et al, 2021;Kukhtyn et al, 2022). Крім того, поряд із визначеннями бактерицидної дії розчинів дезінфікуючих засобів важливе значення має проведення токсикологічних досліджень, які мають на меті встановити клас токсичності та умови використання деззасобу на практиці (Silva et al, 2018;Kovalenko et al, 2018;Palii et al, 2020;Kukhtyn et al, 2020).…”