Evaluation of dimethoate toxicity on fertilization and on embryonic development of Paracentrotus lividus (Lamarck, 1816)

Abstract

Organophosphates are a large class of chemicals with anticholinesterase action insecticides. Dimethoate belongs to the class of organophosphates and it is used for agriculture purpose. Its main toxicological role in animals and humans is the inhibition of the activity of acetylcholinesterase. Although it is not considered genotoxic, carcinogenic and teratogen, there is evidence of increased pup mortality in developmental neurotoxicity studies. Since there is scant published literature about developmental toxicity, we investigated the adverse effects of dimethoate on fertilization and embryonic development in sea urchin (Paracentrotus lividus), a model organism widely used to assess the toxicity of contaminants on environmental matrices; so pesticide residues can be released into the environment, and could affect the health of organisms, including humans. Different solution of dimethoate (4 × 10−3, 4 × 10−4, 4 × 10−5, 4 × 10−6 and 4 × 10−7 g/10 ml) have been tested on spermatozoa of P. lividus to evaluate the fertilizing ability of them when we added egg cells untreated. We demonstrated that dimethoate does not interfere with fertilizing ability of spermatozoa but egg cells fertilized by treated spermatozoa showed alterations in the segmentation planes as asymmetric and/or asynchronous cell divisions.

Recent Advances on Detection of Insecticides Using Optical Sensors

“…Randomly, the application for all these insecticides are used for mosquito control [ 217 ], control a variety of insects that attack fruits, vegetables, landscaping plants and shrubs and also act as acarida [ 218 , 219 ]. It also can be used on pets to control ticks and insects, such as fleas and ants [ 220 , 221 , 222 , 223 , 224 , 225 , 226 , 227 , 228 ].…”

A new approach for reducing pollutants level: a longitudinal cohort study of physical exercises in young people

“…However, many studies have shown adverse effects on mammalian [6][7][8] . Several studies have documented that the carcinogenicity [9] and developmental toxicity [10,11] of dimethoate which can lead to a reduction in plasma cholinesterase [12] . Likewise, BaP has been characterized as a stronger carcinogen than other PAHs compounds [13] which can cause DNA damage and plays a role in lung carcinogenesis [14], atherosclerosis [15] and so on [16][17][18].…”

Oxidative stress, DNA damage, and histological alterations in Bombyx mori exposed orally to pesticide dimethoate

“…Aquatic fauna exposed to dimethoate exhibited muscular tetany, loss of buoyancy ( Heteropneustes fossilis , cat fish: Pandey et al, 2009; Srivastava et al, 2010). , erratic swimming, decreased rate of opercular movement, copious mucus secretion, reduced agility, loss of balance (common carp, Cyprinus carpio : Singh et al, 2009), DNA damage, LPO and ROS stress in brain and liver tissues (Rainbow trout, Oncorhynchus mykiss : Dogan et al, 2011), developmental defects, and so forth (sea urchins Paracentrotus lividus : Scalisi et al, 2020).…”