Evaluation of dilute acid and alkaline pretreatments, enzymatic hydrolysis and fermentation of napiergrass for fuel ethanol production

Camesasca, Laura; Ramírez, María Belén; Guigou, Mairan; Ferrari, Mario Daniel; Lareo, Claudia

doi:10.1016/j.biombioe.2015.01.017

Cited by 77 publications

(54 citation statements)

References 32 publications

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“…The highest ethanol concentration obtained was 13.68 g/L for the SSF time of 120 hours and the sulfuric acid concentration of 3 %. Similar results (15.8 g/L ethanol) were obtained by previous study [16].…”

Section: Effect Of Sulfuric Acid Concentration On Bioethanol Concentrsupporting

confidence: 91%

“…To enhance the biomass conversion, the other authors [14]- [16] studied the combination of acid alkaline pretreatments to produce the bioethanol from the other materials (bamboo, napiergras, sugarcane bagasse). In the other hand, previous researchers [17] investigated the two-steps of rice straw pretreated via aqueous ammonia and dilute sulfuric acid.…”

Section: Introductionmentioning

confidence: 99%

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Bioethanol production from sodium hydroxide – dilute sulfuric acid pretreatment of rice husk via simultaneous saccharification and fermentation

2017

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Abstract. Degradation of lignin from lignocellulosic biomass plays a main key role in converting of lignocelluloses to bioethanol. The pretreatment of lignocelluloses is needed to enhance the fermentable sugars production from enzymatic hydrolysis. This research studied the effect of dilute sulfuric acid pretreatment on the ethanol production using alkaline-dilute acid pretreatment. This research used two stages of the pretreatment process. The first stage used NaOH solution with ratio of rice husk and NaOH solution (1:10 w/v). On the second stage, five concentrations of sulfuric acid of 1 to 5% at temperature of 121 o C and various heating times (30-90 min) were investigated. The ethanol production from pretreated rice husk was conducted by the simultaneous saccharification and fermentation (SSF) for various SSF times of 72-160 hr. The experimental results indicated that an increase in concentration of sulfuric acid capable to destroy the lignocellulosic structure of rice husk. The highest value of cellulose content was achieved about 88.84 % for acid pretreated material at H 2 SO 4 concentration of 2% in 90 minutes. The lignin reduction ranged from 47.46% to 79.29%. The highest ethanol concentration obtained was 13.68 g/L for the SSF time of 120 hours and the sulfuric acid concentration of 3 %.

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Section: Effect Of Sulfuric Acid Concentration On Bioethanol Concentrsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Bioethanol production from sodium hydroxide – dilute sulfuric acid pretreatment of rice husk via simultaneous saccharification and fermentation

2017

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“…For example, the diluted acid pretreatment can effectively hydrolyze hemicellulose, while the pretreatment with alkaline solution can dissolve lignin from the Napier grass. An additional alkaline pretreatment applied to the remaining solid fraction from the diluted acid pretreatment can improve the lignin removal rate …”

Section: Introductionmentioning

confidence: 99%

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai

Lee

Kuan

et al. 2018

Energy Science & Engineering

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Due to its fast‐growing and high carbohydrate content, Napier grass has a great potential to be chosen as a raw material for renewable energy production. The Napier grass after different pretreatments was tested for simultaneous saccharification and fermentation (SSF) with dried yeast (Saccharomyces cerevisiae) and cellulase (CTec2) to produce ethanol. For alkaline pretreatment, the grass was incubated with 10% NaOH at a ratio of 1:20 (w/v) at 90°C for 1 hour. For dilute acid pretreatment, the grass was soaked with 1% H2SO4 at a ratio of 1:10 (w/v) and incubated at 120°C for 1 hour. For the two‐stage pretreatment, the resultant solid from dilute acid pretreatment was further treated with 2% NaOH (1:10 w/v) at 80°C for 6 hours. After enzymatic saccharification at 50°C and pH 5.0 for 96 hours using CTec2, yields of glucose from three pretreated Napier grass samples (10% water‐insoluble solids, WIS) were 75.4 ± 2.9%, 55.4 ± 6.5%, and 76.4 ± 5.0%. Using 10% WIS of alkaline‐pretreated Napier grass biomass as the substrate, the 72‐hour SSF led to an ethanol yield of 86.6 ± 3.4%. On the basis of the dried biomass of Napier grass as the raw material, the ethanol yield could reach 0.143 ± 0.006 (g/g). Napier grass biomass could be effectively treated by alkaline, dilute acid, or two‐stage pretreatment methods to remove non‐cellulosic components to some extent. Alkaline pretreatment was found to be superior to dilute acid and two‐stage pretreatment methods, based on ethanol yields obtained under similar SSF conditions.

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“…La eliminación de lignina modifica la estructura de la madera creando poros o aumentando el tamaño de los ya existentes, lo que produce una organización más abierta y accesible a la penetración de enzimas en la etapa de sacarificación. Camesasca et al (2015) obtuvieron 0.024 g glucosa/g pasto y bajos niveles de subproductos inhibidores con ácido sulfúrico a 1.5 % durante 60 min a 121 ºC. Este valor es bajo comparado con el reportado en este trabajo (1.81 g/L corresponde a 0.077 ± 0.014 g azúcares/g aserrín en los puntos centrales del diseño, según la cantidad de material sacarificado).…”

Section: Pretratamientounclassified

“…Sin embargo, lo anterior puede ocasionar un aumento en la concentración de subproductos inhibidores del proceso fermentativo como furfural y 5-hidroximetilfurfural (Saha et al 2005, Camesasca et al 2015, Martínez et al 2015. El contenido de lignina soluble se midió en el licor resultante del PT2 debido a que la lignina se solubiliza en solución alcalina acuosa (Jagtap et al 2013).…”

Section: Pretratamientounclassified

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

Rodríguez

Castrillo

Velázquez

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: residuo lignocelulósico, diseño factorial, lignina, sacarificación. RESUMENEl uso de residuos lignocelulósicos constituye una fuente abundante y de bajo costo, que genera, además, una solución al problema de su disposición final. El objetivo del trabajo fue evaluar el efecto de distintas condiciones de pretratamiento secuencial ácido-base de aserrín de pino, sobre la liberación de azúcares reductores en la etapa de sacarificación. El material lignocelulósico se pretrató en dos etapas: la primera, se realizó con ácido sulfúrico (PT1) y la segunda con hidróxido de sodio (PT2), ambas con tres niveles: 1, 2 y 3 % mediante un diseño 3 2 factorial. La hidrólisis enzimática se realizó a 50 ºC y 150 rpm en frascos de 50 mL. Se tomaron muestras a las 0.15, 24, 48, 72 y 96 h. Se determinó el porcentaje de lignina ácido soluble y la concentración de azúcares reductores. El nivel óptimo de cada factor que favoreció la mayor deslignificación correspondió a 3 % de ácido sulfúrico y 3 % de hidróxido de sodio. La producción de azúcares reductores no se vio afectada por el PT2, pero sí por el PT1, alcanzando un máximo para el nivel 0 del mismo (2 % de ácido sulfúrico). Se optimizó el pretratamiento secuencial ácido-base y se comprobó además que éste mejora la hidrólisis enzimática respecto al aserrín de pino sin pretratar. La cantidad de azúcares reductores obtenidos fue comparable a la obtenida por otros autores.Key words: lignocellulosic waste, factorial design, lignin, saccharification. ABSTRACTThe use of lignocellulosic waste constitutes an abundant and inexpensive source; it also offers a solution to the problem of waste disposal. The aim of the study was to evaluate the effect of different conditions of a sequential acid-base pretreatment of pine sawdust, through the release of reducing sugars in the saccharification step. The lignocellulosic material was pretreated in two stages: acid pretreatment (PT1), using sulfuric acid and basic pretreatment (PT2), employing sodium hydroxide at three levels each one: 1, 2 and 3 % in a factorial design. Enzymatic hydrolysis was carried out at 50 ºC and 150 rpm in 50 mL vials. Samples were taken at 0.15, 24, 48, 72 and 96 h. The percentage of acid soluble lignin and the concentration of reducing sugars were determined. The Rev. Int.

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Evaluation of dilute acid and alkaline pretreatments, enzymatic hydrolysis and fermentation of napiergrass for fuel ethanol production

Cited by 77 publications

References 32 publications

Bioethanol production from sodium hydroxide – dilute sulfuric acid pretreatment of rice husk via simultaneous saccharification and fermentation

Bioethanol production from sodium hydroxide – dilute sulfuric acid pretreatment of rice husk via simultaneous saccharification and fermentation

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Obtención De Azúcares Fermentables a Partir De Aserrín De Pino Pretratado Secuencialmente Con Ácido-Base

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