Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces

Abstract

We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone-water, Trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and Pril-xylose-ampicillin agar. For Plesiomonas sp., alkaline peptone-water and inositol-brilliant green-bile salts agar were optimal. Four strains of Aeromonas spp. were detected in patient samples with these media.

Clinical significance of Aeromonas species isolated from patients with diarrhea

“…Results of studies of ampicillin susceptibility in Aeromonas spp. indicate that most strains are resistant to a concentration of 10 ,ug/ml (22), whereas higher concentrations recommended by some investigators may be inhibitory to a significant percentage of strains (26). Since completion of this study, the use of MTA and IBG was discontinued because it was nonproductive, despite published recommendations (2,26).…”

Bacteriological eutrophication indicators in four Colombian water bodies (South America)

“…The techniques employed followed those outlined by APHA (1992 Graevenitz & Bucher 1983) was used as an enrichment medium, and starch ampicillin agar as a selective and differential culture medium (Palumbo et al 1985). The cultures were incubated at 28°C for 24 and 48 h. Plesiomonas shigelloides was isolated in APW, pH 8.5 and IBB agar (inositol-brilliant green-bile salts agar; Von Graevenitz & Bucher 1983); the incubation temperature was 44°C for 24 and 48 h. In the case of Pseudomonas spp., the primary isolation was done with Malachite Green Broth (Merck 1396, Darmstadt, Germany), and Cetrimide agar (Merck 5284) as a selective and differential media, incubating at 35°C for 24 and 48 h. Counting of bacteria in water samples was performed by the membrane filtration technique (APHA 1992), and for the sediment samples the spread plate method. In all cases, the confirmation of suspect colonies was done using Gram staining, pigment production, oxidase test, and typical growth in each selective agar.…”

AEROMONAS HYDROPHILA and AEROMONAS SOBRIA AS POTENTIAL FOOD POISONING SPECIES: A REVIEW1

“…von Graevenitz and Bucher (1983) evaluated nine plating media and enrichment broths for their ability to detect A. hydrophila in stool specimens and correctly differentiated them from Enterobacteriaceae and Pleisiomonas. They recommended the use of alkaline peptone water as an enrichment, and either inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, or Pril-xylose-ampicillin agar as a differential plating medium.…”