Evaluation of Different Parasitological Techniques for Diagnosing Intestinal Parasites in Dogs

Santana, Breno Barros de; Silva, Tallys Leandro Barbosa da; Ramos, Rafael Antonio Nascimento; Alves, Lêucio Câmara; Carvalho, Gílcia Aparecida de

doi:10.4236/ojvm.2015.52003

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Previous studies demonstrated a wide variation ranging from 1.2% (Castro et al, 2005) to 24.2% (Capuano and Rocha 2006) in the frequency of this nematode in fecal samples collected from the environment. This variation may occur due to the period (wet or dry) in which samples were collected, or due to the different methods of retrieval of eggs (Santana et al, 2015). It is important to highlight that Ancylostoma spp.…”

Section: Discussionmentioning

confidence: 99%

Parasites with zoonotic potential in canine fecal samples from Garanhuns, Pernambuco, Brazil

et al. 2019

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Among the gastrointestinal parasites of dogs that present public health importance, the species of the genus Ancylostoma and Toxocara are the most frequently detected, and have been important cause of soil contamination. The aim of this study was to detect gastrointestinal parasites in dog's feces collected in households, streets and public squares of the city of Garanhuns, Pernambuco, Brazil. Fecal samples (n = 640) were collected in the environment and evaluated by parasitological methods of Willis-Mollay and Hoffman, Pons and Janer. Out of all samples, 51.09% (327/640) were positive for gastrointestinal parasites. In particular, in 70.94% (232/327) of the samples were detected eggs of Ancylostoma spp., and in 11.01% (36/327) coinfection by Ancylostoma spp. and Toxocara canis. In conclusion, the presence of eggs of zoonotic parasites in feces collected from the environment in the city of Garanhuns represent a risk for the transmission of Cutaneous Larva Migrans and Visceral Larva Migrans for the human population. ResumoDentre os parasitos gastrintestinais de cães com importância em saúde pública, as espécies dos gêneros Ancylostoma e Toxocara são as mais frequentemente encontradas, e contaminam o solo. O objetivo desse estudo foi detectar parasitos gastrintestinais em fezes de cães coletadas em domicílios, ruas e praças públicas da cidade de Garanhuns, Pernambuco, Brasil. Amostras fecais (n = 640) foram coletadas no ambiente e avaliadas pelos métodos parasitológicos de Willis-Mollay e Hoffman, Pons and Janer. De todas as amostras, 51,09% (327/640) foram positivas para parasitos gastrintestinais. Em particular, em 70,94% (232/327) das amostras foram detectados ovos de Ancylostoma spp., e em 11,01% (36/327) coinfecção por Ancylostoma spp. e Toxocara canis. Em conclusão, a presença de ovos de parasitos zoonóticos em fezes coletadas no ambiente na cidade de Garanhuns representa risco para a transmissão de Larva Migrans Cutanea e Larva Migrans Visceral para a população humana.Palavras-chave: Ancylostoma spp.; Toxocara canis; zoonose.

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Section: Discussionmentioning

confidence: 99%

Parasites with zoonotic potential in canine fecal samples from Garanhuns, Pernambuco, Brazil

et al. 2019

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“…The techniques most frequently used are Flotation in Saturated Sodium Chloride Solution (25), Centrifugal Flotation in Saturated Zinc Sulfate Solution (26) and Spontaneous Sedimentation (27). These techniques are used mainly due to their low cost and because they are practical and direct (13,(28)(29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 99%

Automated Diagnostics: Advances in the Diagnosis of Intestinal Parasitic Infections in Humans and Animals

et al. 2021

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The increasingly close proximity between people and animals is of great concern for public health, given the risk of exposure to infectious diseases transmitted through animals, which are carriers of more than 60 zoonotic agents. These diseases, which are included in the list of Neglected Tropical Diseases, cause losses in countries with tropical and subtropical climates, and in regions with temperate climates. Indeed, they affect more than a billion people around the world, a large proportion of which are infected by one or more parasitic helminths, causing annual losses of billions of dollars. Several studies are being conducted in search for differentiated, more sensitive diagnostics with fewer errors. These studies, which involve the automated examination of intestinal parasites, still face challenges that must be overcome in order to ensure the proper identification of parasites. This includes a protocol that allows for elimination of most of the debris in samples, satisfactory staining of parasite structures, and a robust image database. Our objective here is therefore to offer a critical description of the techniques currently in use for the automated diagnosis of intestinal parasites in fecal samples, as well as advances in these techniques.

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“…However, it should be noted that other authors have detected different results. According to Santana et al (2015), the centrifugal flotation technique showed higher sensitivity towards detecting Ancylostoma spp. and Toxocara spp.…”

Section: Introductionmentioning

confidence: 99%

Helminth eggs with zoonotic potential in the vicinity of public schools in southern Brazil

Mello

Nizoli

Ferraz

et al. 2020

Rev. Bras. Parasitol. Vet.

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The aim of this study was to evaluate environmental contamination by helminth eggs with zoonotic potential that were found in dog feces in the vicinity of elementary schools. Seventy-nine samples of dog feces were collected from 28 municipal schools located in five neighborhoods in Pelotas, Rio Grande do Sul (RS), Brazil. The samples were processed using the Willis-Mollay technique and analyzed using an optical microscope (40X), to identify any parasite eggs present. All neighborhoods were positive and 74.7% of the samples exhibited one or more helminth genera. The agent with the highest prevalence was Ancylostoma spp. (93.2%), followed by Trichuris spp. (18.6%), Toxocara spp. (11.9%) and Toxascaris (1.7%). These data show that there is a need for greater care towards controlling these helminths with zoonotic potential, including responsible pet ownership and daily activities to clean and collect dog feces in the vicinity of schools, because these are places where children play and study.

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Evaluation of Different Parasitological Techniques for Diagnosing Intestinal Parasites in Dogs

Cited by 6 publications

References 12 publications

Parasites with zoonotic potential in canine fecal samples from Garanhuns, Pernambuco, Brazil

Parasites with zoonotic potential in canine fecal samples from Garanhuns, Pernambuco, Brazil

Automated Diagnostics: Advances in the Diagnosis of Intestinal Parasitic Infections in Humans and Animals

Helminth eggs with zoonotic potential in the vicinity of public schools in southern Brazil

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