Evaluation of different methods for detecting circulating immune complexes. Studies in patients with lung cancer

Füst, George; Fekete, B.; Angyal, I.; Jakab, Ágnes; Pal, A. K.; Merétey, K; Falus, András; Török, Katalin; Szegedi, G; Kávai, M; Puskás, E; Csécsi-Nagy, Mária; Szabó, Tamás; Lenkey, Ágota; Misz, M

doi:10.1016/0022-1759(81)90311-2

Cited by 13 publications

(4 citation statements)

References 20 publications

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“…Because of the heterogeneity of CICs, parallel application of methods based on different principles may be a useful approach [6,8,11,131. Collective experience in the measurement of CIC has shown that no single method is ideal in all circumstances and that valuable information can be derived from the use of two or more test systems [13,22,29].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Patel

Gopal

Nadkarni

1985

Cancer Immunol Immunother

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Circulating immune complexes (CICs) have been detected in the sera of patients with non-Hodgkin's lymphoma (NHL), Hodgkin's disease, chronic myeloid leukemia, and acute lymphoblastic leukemia by using C1q-binding and L1210-binding assays. Both assays gave broadly similar patterns of reactivity in terms of frequency and magnitude, though there are some differences. Significantly elevated CIC levels were observed in all pathologic groups. However, sera from NHL patients with an unfavorable prognosis consistently exhibited the highest frequency of positive values and mean CIC levels in both these assays. The two tests showed concordance in 66.6% of the NHL patients' sera and were significantly correlated. Of the sera from NHL patients 12.7% were positive in the C1q-binding assay only and 15.9% in the L1210-binding assay only. Both the assays gave positive results in some patients, and a degree of overlap indicates the presence of different types of CIC in cancer patients' sera. The combined use of two methods for detecting CICs may be useful for evaluation of the activity, the extent, and the prognosis of the malignant disease.

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Section: Discussionmentioning

confidence: 99%

“…This makes the clinical usefulness of determining CIC in malignant diseases doubtful. A comparison of various methods quantifying reference preparations of aggregated immunoglobulins showed a good correlation between some of these tests, however [3,5,6,8,9,13]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Patel

Gopal

Nadkarni

1985

Cancer Immunol Immunother

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“…A number of tests (24) including several solidphase enzyme immunoassays (2,3,15,18,19,22) have been devised for the detection of CIC. However, when different assays are compared, the correlation among the results is rather poor (5,6).…”

Section: Discussionmentioning

confidence: 99%

“…For some tests, proper standard curves cannot be constructed, while others discriminate poorly between patient and control series (1 2). Frequently, there is a lack of correlation among different tests (6,26), and in most assays, several factors may interfere with the results and provide false positive or false negative results (16,25,27).…”

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confidence: 99%

Problems in the Detection of Complement‐fixing Immune Complexes

Rødahl¹

1985

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

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In the application of the anti‐C1q, the anti‐C3, or the conglutinin‐binding assay for the detection of complement‐fixing circulating immune complexes in patients with ankylosing spondylitis or healthy blood donors, antibodies cross‐reacting with the solid‐phase bound agent were observed in several sera, resulting in erroneous interpretation of the tests. Corrections for the interfering antibodies were made by testing for the binding to conglutinin both in the presence and in the absence of Ca**, while the anti‐C1q and the anti‐C3 assays included the application of F(ab')2 fragments of normal IgG to check for anti‐F(ab')2 anti‐bodies.

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Primary lung cancer.A controlled study of preoperative and postoperative levels of circulating immune complexes

Bukh

Aguado

Kimose

et al. 1988

Cancer

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Three complement-dependent and one rheumatoid factor-dependent immune complex assays were used to analyze the sera taken before surgery from 70 unselected and previously untreated lung cancer patients, from 30 of them after surgery, and from 31 healthy controls. Plasma levels of complement split product C3d were also analyzed. The levels of circulating immune complexes (cIC) and C3d were essentially the same in samples taken from lung cancer patients before surgery and from healthy controls. By the four immune complex assays, increased levels were found in 0 to 10% of the preoperative lung cancer patients compared to 3% of the healthy controls (not significant). The postsurgical tumor, lymph node, metastasis (pTNM) stage of the lung cancer was not reflected in the levels of cIC or C3d. Paired comparisons of the cIC and C3d levels before and after surgery did not show significant differences. Thus, we found no evidence for the occurrence of cancer-related cIC in lung cancer patients.

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Evaluation of different methods for detecting circulating immune complexes. Studies in patients with lung cancer

Cited by 13 publications

References 20 publications

Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Problems in the Detection of Complement‐fixing Immune Complexes

Primary lung cancer.A controlled study of preoperative and postoperative levels of circulating immune complexes

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