Evaluation of different methods for detecting circulating immune complexes. An inter-laboratory study

Füst, George; Kávai, M; Szegedi, G; Merétey, K; Falus, András; Lenkey, Ágota; Misz, M

doi:10.1016/0022-1759(80)90276-8

Cited by 28 publications

(7 citation statements)

References 9 publications

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“…The expression of FC7RI was elevated in parallel with the serum IC level of the patients. It is well known that the different methods for determination of serum IC detect different types of immune complexes in the blood of SLE patients [9]. In this study we chose the photometry and functional tests, because a significant positive correlation had been obtained earlier between their results [9]., as well as a correlation between the serum IC level detected by the functional test and the total uptake of sensitized erythrocytes by SLE monocytes [2].…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that the different methods for determination of serum IC detect different types of immune complexes in the blood of SLE patients [9]. In this study we chose the photometry and functional tests, because a significant positive correlation had been obtained earlier between their results [9]., as well as a correlation between the serum IC level detected by the functional test and the total uptake of sensitized erythrocytes by SLE monocytes [2]. Among the FC7RS the FC7RI is the most flexible, it is almost the only FC7R whose expression can be exclusively induced by other factors such as interleukin-1 and gamma-interferon [11].…”

Section: Discussionmentioning

confidence: 99%

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Correlations of Monocyte Phagocytic Receptor Expressions with Serum Immune Complex Level in Systemic Lupus Erythematosus

et al. 1994

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The expression of Fc gamma RI, Fc gamma RII, and Fc gamma RIII (the IgG receptors CD64, CD32, CD16) as well as CR3 (the C3bi receptor, CD11b) on monocytes in the blood of patients with systemic lupus erythematosus (SLE) was investigated. The relationship between the receptor expression and the serum immune complex (IC) concentration was analysed. The decrease in mean fluorescence intensity (FI) of the Fc gamma RII of patients' monocytes stained by specific monoclonal antibodies (MoAb IV3) was very close to statistical significance (P = 0.052). The expression (FI) of CR3 (using MoAb OKM1) on monocytes of patients was also decreased, but not significantly. The detected decrease of Fc gamma RII and CR3 was inversely correlated with the high circulating immune complex level in patients' sera. At the same time, Fc gamma RI expression on SLE monocytes (using MoAb 32) was significantly elevated and this change was in parallel with the serum IC concentration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Correlations of Monocyte Phagocytic Receptor Expressions with Serum Immune Complex Level in Systemic Lupus Erythematosus

et al. 1994

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“…This makes the clinical usefulness of determining CIC in malignant diseases doubtful. A comparison of various methods quantifying reference preparations of aggregated immunoglobulins showed a good correlation between some of these tests, however [3,5,6,8,9,13]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Patel

Gopal

Nadkarni

1985

Cancer Immunol Immunother

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Circulating immune complexes (CICs) have been detected in the sera of patients with non-Hodgkin's lymphoma (NHL), Hodgkin's disease, chronic myeloid leukemia, and acute lymphoblastic leukemia by using C1q-binding and L1210-binding assays. Both assays gave broadly similar patterns of reactivity in terms of frequency and magnitude, though there are some differences. Significantly elevated CIC levels were observed in all pathologic groups. However, sera from NHL patients with an unfavorable prognosis consistently exhibited the highest frequency of positive values and mean CIC levels in both these assays. The two tests showed concordance in 66.6% of the NHL patients' sera and were significantly correlated. Of the sera from NHL patients 12.7% were positive in the C1q-binding assay only and 15.9% in the L1210-binding assay only. Both the assays gave positive results in some patients, and a degree of overlap indicates the presence of different types of CIC in cancer patients' sera. The combined use of two methods for detecting CICs may be useful for evaluation of the activity, the extent, and the prognosis of the malignant disease.

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“…This fact can mean that a broadspectrum of immune complexes with different properties can circulate in the patient's blood. These complexes may differ from each other in size, in composition, or in the nature of antigen and antibody components [27].…”

Section: Discussionmentioning

confidence: 99%

Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

et al. 1996

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Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl $300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.

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Evaluation of different methods for detecting circulating immune complexes. An inter-laboratory study

Cited by 28 publications

References 9 publications

Correlations of Monocyte Phagocytic Receptor Expressions with Serum Immune Complex Level in Systemic Lupus Erythematosus

Correlations of Monocyte Phagocytic Receptor Expressions with Serum Immune Complex Level in Systemic Lupus Erythematosus

Evaluation of circulating immune complexes in lymphomas and leukemias using two different assays

Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

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