Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

Abstract

Cellulases (enzymes of great potential in biotechnology) are currently of interest due to their applicability in the hydrolysis of cellulose from lignocellulosic materials, producing fermentable sugars for alcohol production. The great demand for efficient enzymes is the driving force to prospect new cellulases-producing microorganisms, as well as to optimize the enzyme production step. Many variables can be optimized in microorganism cultivation, such as pH, type of microorganism, induction, temperature, type and concentration of substrate, among others. This work aimed to evaluate the production of cellulases by submerged fermentation from three strains of filamentous fungi (Trichoderma koningii, Penicillium species, Rhizomucor species) and two strains of bacteria (Bacillus megaterium and Bacillus subtilis), using sugarcane bagasse as substrate. Variations in substrate concentrations (0.5, 1.6 and 2.7%, w/v) and temperature (28, 33 and 38°C) were evaluated on volumetric activity. The best fungus was T. koningii (3130.4 IU/L) using 2.7% natural sugarcane bagasse at 28°C. Among the bacteria, B. megaterium stood out with an enzyme production in range of 130 to 156.7 IU/L (at 28-33°C using natural and acid-alkaline pretreated bagasses), although up to around 20 times lower than the production by the T. Koningii.

Analytical study on Fungal Cellulase Produced by Penicillium Expansum grown on Malus Domestica (Apple Fruits)

“…An attempt to solve this has led to increase in demand for enzymes to catalyze several industrial processes (Jadhav et al, 2013;Saratale et al, 2014;Mostafa et al, 2019). Microbial enzymes essentially fungal enzymes have been found applicable in various commercial production processes (Pinotti et al, 2020). Due to the importance associated with enzymes of microbial origin, selected microorganisms including bacteria, moulds, yeasts and insects have been globally studied for the biosynthesis of economically viable preparations of various enzymes for commercial applications (Chirumamilla et al, 2001;Jadhav et al, 2013;Behera et al, 2017;Banerjee et al, 2020).…”

“…They are also used in animal feeds, in processing of fruit juice and in baking. Cellulases are also of immense importance in bioconversion of renewable organic biomass to usable biofuels and inorganic chemicals (Lynd et al, 2005;Imram et al, 2016;Sajith et al, 2016;Pinotti et al, 2020).…”