Evaluation of Different Extraction Methods for the Analysis of Per‐ and Polyfluoroalkyl Substances in Dried Blood Spots from the Florida Manatee (<i>Trichechus manatus</i>)

Griffin, Emily K.; Aristizabal‐Henao, Juan J.; Bowden, John A.

doi:10.1002/etc.5175

Cited by 6 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous research has measured elevated PFAS and other pollutants in aquatic herbivores, ,,− yet the route of exposure in these individuals is unknown. The results of the present study provide evidence for AV as a significant route of PFAS exposure in aquatic herbivores.…”

Section: Resultsmentioning

confidence: 99%

Aquatic Vegetation, an Understudied Depot for PFAS

Griffin

Hall

Brown

et al. 2023

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

Per- and polyfluoroalkyl substances (PFAS) are a class of manufactured chemicals that have been extensively utilized worldwide. We hypothesize that the presence, uptake, and accumulation of PFAS in aquatic vegetation (AV) is dependent upon several factors, such as the physiochemical properties of PFAS and proximity to potential sources. In this study, AV was collected from eight locations in Florida to investigate the PFAS presence, accumulation, and spatiotemporal distribution. PFAS were detected in AV at all sampling locations, with a range from 0.18 to 55 ng/g sum (∑)PFAS. Individual PFAS and their concentrations varied by sampling location, time, and AV species. A total of 12 PFAS were identified, with the greatest concentrations measured in macroalgae. The average bioconcentration factor (BCF) among all samples was 1225, indicating high PFAS accumulation in AV from surface water. The highest concentrations, across all AV types, were recorded in the Indian River Lagoon (IRL), a location with a history of elevated PFAS burdens. The present study represents the first investigation of PFAS in naturally existing estuarine AV, filling an important gap on PFAS partitioning within the environment, as well as providing insights into exposure pathways for aquatic herbivores. Examining the presence, fate, and transport of these persistent chemicals in Florida’s waterways is critical for understanding their effect on environmental, wildlife, and human health.

show abstract

Section: Resultsmentioning

confidence: 99%

Aquatic Vegetation, an Understudied Depot for PFAS

Griffin

Hall

Brown

et al. 2023

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Under similar research the most dangerous factors affecting the biological activity and birds health caused by human use of pesticides, insecticides, antibiotics, heavy metals and other xenobiotics can be identified (Trudeau et al 2007 ; Shlosberg et al 2011 , 2012 ; Lehner et al 2013 , 2018 , 2020 ; Perkins and Basu 2018 ). Marine mammals are also at risk to be exposured to mercury (Hansen et al 2014 ) and other environmental anthropogenic contaminants (Griffin et al 2021 ), all these arise concern for potential adverse effects on wildlife populations. Thus, McHuron et al ( 2019 ) made a risk assessment of mercury concentration in hair, blood and DBS of marine wild-caught pinnipeds.…”

Section: Dbs Samples In Veterinary Medicine and Animal Biology: Diagn...mentioning

confidence: 99%

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

View full text Add to dashboard Cite

Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

show abstract

Development and validation of the UHPLC-MS/MS method for the quantitative determination of 25 PFAS in dried blood spots

Galletto,

Ververi,

Massano

et al. 2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Per- and polyfluoroalkyl substances (PFAS) are anthropogenic fluorine-containing compounds largely used in industrial and consumer applications. They tend to bioaccumulate in the human body after intake from various sources in daily life. Following repeated exposure to PFAS, a broad range of adverse health outcomes has been reported. Consequently, monitoring PFAS levels in human blood is of paramount importance for public health policies. In contrast with traditional venipuncture, dried blood spots (DBS) constitute a reliable, cheap, and less invasive technique to allow microsampling by capillary blood collected on a specific device. This work aimed to develop and validate an innovative analytical method, combining quantitative DBS with UHPLC-MS/MS instrumentation to identify and quantify 25 PFAS. The extraction procedure was developed and optimized within the range 2–100 ng/mL. Specifically, fortified blood was applied on Capitainer®B devices providing 10 μL of blood volume through a microfluidic channel. After 3 h of drying, the extraction was performed by methanol under sonication, followed by centrifugation. Then, the extraction solvent was evaporated; the residue was reconstituted with the mobile phase solution. The validated method evidenced good sensitivity, with limits of detection ranging from 0.4 ng/mL (PFODA, PFOS) to 1.0 ng/mL (PFOA, 3,6-OPFHpA). The ± 20% acceptability criteria established for intra- and inter-day precision and accuracy were fulfilled for all analytes. High recovery—above 80%—was recorded, whereas significant matrix effect resulted in ion enhancement (> 50%) for 13 analytes. In conclusion, the proposed workflow proved to be reliable, fit for purpose, and easily adaptable in the laboratory routine. Graphical Abstract

show abstract

Evaluation of Different Extraction Methods for the Analysis of Per‐ and Polyfluoroalkyl Substances in Dried Blood Spots from the Florida Manatee (Trichechus manatus)

Cited by 6 publications

References 47 publications

Aquatic Vegetation, an Understudied Depot for PFAS

Aquatic Vegetation, an Understudied Depot for PFAS

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Development and validation of the UHPLC-MS/MS method for the quantitative determination of 25 PFAS in dried blood spots

Contact Info

Product

Resources

About