Evaluation of different agricultural wastes for the production of fruiting bodies and bioactive compounds by medicinal mushroom <i>Cordyceps militaris</i>

Lin, Qunying; Long, Liangkun; Wu, Liang-Liang; Zhang, Fenglun; Wu, Shuling; Zhang, Weiming; Sun, X.-D.

doi:10.1002/jsfa.8097

Cited by 38 publications

(21 citation statements)

References 17 publications

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“…Dong et al [ 41 ] reported that environmental conditions affected the accumulation of active components and the biological efficiency of Cordyceps militaris . Lin et al [ 42 ] showed that the substrate not only affects the yield of C . militaris , but also the production of medicinally active components.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage

Liu

Xie

et al. 2018

PLoS ONE

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Ganoderma lucidum is a medicinal mushroom that has been widely used in East Asia for the treatment of various diseases. The pharmacological activity of this fungus is primarily attributable to the polysaccharides and triterpenoids. In this study, to obtain the fruit bodies with improved content of active constituents, we examined the effect of salicylic acid (SA) and calcium ion on the biosynthesis of polysaccharides and triterpenoids by spraying the chemicals during the fruiting. To explore the underlying mechanisms for the variation, the transcripts of related genes involved in the polysaccharide and triterpenoid biosynthesis were measured. Results showed that Ca2+ had no effect on production of polysaccharides and triterpenoids, whereas SA increased triterpenoid content by 23.32%, compared to the control, but it had little influence on polysaccharide production. Interestingly, the combined induction increased polysaccharide and triterpenoid content by 9.02% and 13.61%, respectively, compared to the control. Under Ca2+ induction, the transcript of ugp gene in the polysaccharide biosynthetic pathway up-regulated in all three stages (mycelium, primordium, and fruit body), while pgm and gls gave no response in the mycelium and primordium stages, and up-regulated in the fruit body stage. Differently, six key triterpenoid biosynthetic genes including hmgr, hmgs, mvd, fps, sqs, and ls did not respond to the induction. In the case of SA and combined induction, pgm and ugp were up-regulated in all three stages, while gls showed an increased expression in the primordium stage and no response in other stages. The six triterpenoid biosynthetic genes were up-regulated in all three stages. The present study provides a useful approach to producing G. lucidum fruit bodies with high polysaccharide and triterpenoid content. This is important to the G. lucidum industry.

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Section: Discussionmentioning

confidence: 99%

Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage

Liu

Xie

et al. 2018

PLoS ONE

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“…The poor utilization of these SS has made them a serious environmental problem and increased recovery cost of these materials for the industry. Fortunately, latest researches have recommended the potential use of these SS in animal feeding, recycling substrate and natural active constituents extraction (Doan, Hoseinifar, Tapingkae, Chitmanat, & Mekchay, 2017;Lin et al, 2017; Van, Hoseinifar, Mao, Chitmanat, & Tayyamath, 2017;Wu, Chen, Chang, & Shih, 2013). Thanks to the advantage of the SS in number of applications, compared with CM fruit body, there is a great potential attraction for scientific study and commercial value of SS.…”

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confidence: 99%

Effects of superfine grinding on the properties and qualities of Cordyceps militaris and its spent substrate

Shi

Zhang

Bhandari

et al. 2019

J Food Process Preserv

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The main purpose of this study is to study the effect of superfine grinding (SG) technology on the physicochemical properties of Cordyceps militaris (CM) and its spent substrate (SS). The results showed that the particle size of the sample powder decreased significantly under the SG treatment and reached below 15 μm finally. With the decrease in particle size, the surface tension of materials decreased and water‐holding capacity (WHC) of aqueous solution of CM and its SS powder decreased by 58.72% and 48.36%, while the water soluble index (WSI) increased to 0.73 and 0.44 g/g, respectively, also the hygroscopic property, L* value and DPPH radical scavenging capacity increased significantly. In addition, the cordycepin yield of two materials increased by 9.49% and 14.61% for 4 hr SG treatment, respectively. The results showed that the SG technology could effectively improve the physicochemical properties of CM powder and the extraction of its effective components, in addition, a new idea was opened up for the effective utilization of waste materials such as edible and medicinal fungi culture medium. Practical applicationsThis study investigated the effect of superfine grinding treatments on the physicochemical properties of the powder of Cordyceps militaris (CM) and its spent substrate (SS). The results shows the advances of superfine grinding on the particle size, surface tension, water hydration capacity, color, antioxidant capacity, and the yield of active component. And it could realize the maximum utilization of resources like medical plants and the reutilization of the waste during herb culturing, when treatments like superfine grinding was applied.

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“…However, it is not enough to meet demand for cordycepin. Thus, researches to increase the natural cordycepin content in C. militaris have been peformed through various methods such as breeding by mating or genetic manipulation and C. militaris with higher production of cordycepin is very valuable in the health food or medicine industry [ 9 10 ].…”

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confidence: 99%

Development of High Cordycepin-Producing Cordyceps militaris Strains

et al. 2017

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Cordyceps militaris, known as Dong-Chong-Xia-Cao, produces the most cordycepin among Cordyceps species and can be cultured artificially. For these reasons, C. militaris is widely used as herb or functional food in the East Asia. In this study, we developed a new strain of C. militaris that produces higher cordycepin content than parent strains through mating-based sexual reproduction. Twenty parent strains were collected and identified as C. militaris based on internal trasncrived spacer and rDNA sequences. Seven single spores of MAT 1-1 idiomorph and five single spores of MAT 1-2 idiomorph were isolated from 12 parent strains. When 35 combinations were mated on the brown rice medium with the isolated single spores, eight combinations formed a stroma with a normal perithecia and confirmed mated strains. High pressure liquid chromatography analysis showed that mated strain KSP8 produced the most cordycepin in all the media among all the tested strains. This result showed due to genetic recombination occurring during the sexual reproduction of C. militaris. The development of C. militaris strain with increased cordycepin content by this approach can help not only to generate new C. militaris strains, but also to contribute to the health food or medicine industry.

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Evaluation of different agricultural wastes for the production of fruiting bodies and bioactive compounds by medicinal mushroom Cordyceps militaris

Abstract: CS and CCP are effective substrates for the production of fruit bodies and bioactive compounds by C. militaris. This study provides a new approach to decreasing the cost of C. militaris cultivation and dealing with these agricultural wastes. © 2016 Society of Chemical Industry.

Cited by 38 publications

References 17 publications

Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage

Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage

Effects of superfine grinding on the properties and qualities of Cordyceps militaris and its spent substrate

Development of High Cordycepin-Producing Cordyceps militaris Strains

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