Evaluation of dialysis membrane process for quantifying the in vitro drug-release from colloidal drug carriers

Moreno-Bautista, Gabriel; Tam, Kam Chiu

doi:10.1016/j.colsurfa.2011.07.032

Cited by 65 publications

(40 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is very crucial to select an ideal dialysis membrane with the largest possible pore size to allow free drug diffusion, and yet the pore size must be small enough so that the nanoparticles cannot pass through (27, 30). It has been suggested by Xu et al .…”

Section: Current In Vitro Dissolution/release Testing Methodsmentioning

confidence: 99%

In vitro dissolution testing strategies for nanoparticulate drug delivery systems: recent developments and challenges

Shen

Burgess

2013

Drug Deliv. and Transl. Res.

148

View full text Add to dashboard Cite

Nanoparticulate systems have emerged as prevalent drug delivery systems over the past few decades. These delivery systems (such as liposomes, emulsions, nanocrystals, and polymeric nanocarriers) have been extensively used to improve bioavailability, prolong pharmacological effects, achieve targeted drug delivery, as well as reduce side effects. Considering that any unanticipated change in product performance of such systems may result in toxicity and/or change in vivo efficacy, it is essential to develop suitable in vitro dissolution/release testing methods to ensure product quality and performance, and to assist in product development. The present review provides an overview of the current in vitro dissolution/release testing methods such as dialysis, sample and separate, as well as continuous flow methods. Challenges and future directions in the development of standardized and biorelevant in vitro dissolution/release testing methods for novel nanoparticulate systems are discussed.

show abstract

Section: Current In Vitro Dissolution/release Testing Methodsmentioning

confidence: 99%

In vitro dissolution testing strategies for nanoparticulate drug delivery systems: recent developments and challenges

Shen

Burgess

2013

Drug Deliv. and Transl. Res.

148

View full text Add to dashboard Cite

show abstract

“…A few other groups also reported similar issues with respect to inherent barrier properties of dialysis membrane and drug partitioning between dispersed phases during drug transport processes. 16,17 Hence, compared to dynamic dialysis method, the real-time monitoring using TCSPC technique displayed about 20 ± 5% difference under normal conditions (without enzyme) and 20 ± 1% under enzymatic conditions. The actual drug concentration present in the interior hydrophilic cavities of vesicles and the rate of drug release across the vesicular wall are the main factors to be considered.…”

Section: ■ Introductionmentioning

confidence: 97%

Real-Time Drug Release Analysis of Enzyme and pH Responsive Polysaccharide Nanovesicles

2015

View full text Add to dashboard Cite

The accurate estimation of drug release kinetics of polymeric vehicles is an indispensable prerequisite for the developments of successful drug carriers for cancer therapy. The present investigation reports the development of timeresolved fluorescence spectroscopic approach for the real-time release kinetics of fluorophore loaded polysaccharide vesicles that are potential vectors in cancer treatment. The polysaccharide vesicles were custom designed with appropriate enzyme and pH responsiveness and loaded with water-soluble biocompatible fluorophore Rhodamine B (Rh-B). The semipermeable membrane dialysis method along with steady state absorbance spectroscopic technique was found to be inaccurate for the estimation of drug release. Time correlated single photon counting (TCSPC) technique was found to exhibit significant difference in excited state decay profiles and fluorescent lifetime of Rh-B in the free and polymer bound states. This enabled the establishment of real-time drug release protocols by TCSPC method for polysaccharide vesicles that are responsible to pH and enzyme with respect to intracellular compartments. Real-time analysis predicted the release kinetics 20−25% higher accuracy when compared to the dialysis method under in vitro conditions. Moreover, the ability of enzyme to cleave the polysaccharide vesicles was further validated by docking studies. The positioning of the molecules in active site of enzyme and the binding energy data were generated using AUTODOCK program to study the rupture of polysaccharide vesicles. This new TCSPC technique could be very useful for studying the drug release pattern of synthetic polymer vesicles loaded with Rh-B fluorophore.

show abstract

“…Release studies were performed with free Dox to probe dialysis membrane transport as this membrane poses an additional barrier to diffusion that may impact observed release kinetics (33, 34). Irrespective of pH or formulation, the rate constant for Dox diffusion through the dialysis membrane was ~0.80 hr −1 .…”

Section: Discussionmentioning

confidence: 99%

Release, Partitioning, and Conjugation Stability of Doxorubicin in Polymer Micelles Determined by Mechanistic Modeling

et al. 2014

View full text Add to dashboard Cite

Purpose To better understand the mechanistic parameters that govern drug release from polymer micelles with acid-labile linkers. Methods A mathematical model was developed to describe drug release from block copolymer micelles composed of a poly(ethylene glycol) shell and a poly(aspartate) core, modified with drug binding linkers for pH-controlled release [hydrazide (HYD), aminobenzoate-hydrazide (ABZ), or glycine-hydrazide (GLY)]. Doxorubicin (Dox) was conjugated to the block copolymers through acid-labile hydrazone bonds. The polymer drug conjugates were used to prepare three polymer micelles (HYD-M, ABZ-M, and GLY-M). Drug release studies were performed to identify the factors governing pH-sensitive release of Dox. The effect of prolonged storage of copolymer material on release kinetics was also observed. Results Biphasic drug release kinetics were observed for all three micelle formulations. The developed model was able to quantify observed release kinetics upon the inclusion of terms for unconjugated Dox and two populations of conjugated Dox. Micelle/water partitioning of Dox was also incorporated into the model and found significant in all micelles under neutral conditions but reduced under acidic conditions. The drug binding linker played a major role in drug release as the extent of Dox release at specific time intervals was greater at pH 5.0 than at pH 7.4 (HYD-M > ABZ-M > GLY-M). Mathematical modeling was also able correlate changes in release kinetics with the instability of the hydrazone conjugation of DOX during prolonged storage. Conclusion These results illustrate the potential utility of mechanistic modeling to better assess release characteristics intrinsic to a particular drug/nanoparticle system.

show abstract

Evaluation of dialysis membrane process for quantifying the in vitro drug-release from colloidal drug carriers

Cited by 65 publications

References 21 publications

In vitro dissolution testing strategies for nanoparticulate drug delivery systems: recent developments and challenges

In vitro dissolution testing strategies for nanoparticulate drug delivery systems: recent developments and challenges

Real-Time Drug Release Analysis of Enzyme and pH Responsive Polysaccharide Nanovesicles

Release, Partitioning, and Conjugation Stability of Doxorubicin in Polymer Micelles Determined by Mechanistic Modeling

Contact Info

Product

Resources

About