Evaluation of diagnostic tests used for detection of bovine viral diarrhea virus and prevalence of subtypes 1a, 1b, and 2a in persistently infected cattle entering a feedlot

Fulton, Robert W.; Hessman, Bill E.; Johnson, Bill; Ridpath, Julia F.; Saliki, Jeremiah T.; Burge, Lurinda J.; Sjeklocha, Dave; Confer, Anthony W.; Funk, Rebecca A.; Payton, Mark E.

doi:10.2460/javma.228.4.578

Cited by 85 publications

(83 citation statements)

References 23 publications

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“…The BVDV isolates were typed as BVDV1a, BVDV-1b, BVDV-2a, or BVDV-2b, based on the phylogenetic comparisons of sequences of the 59-untranslated region (UTR) of the viral genome. 7,10,11 Reference BVDV strains were included in these comparisons, including those BVDV strains currently used in viral vaccines. The BVDV IHC testing was performed on formalin-fixed ear notches from 94 cattle in the study.…”

Section: Agent Identificationmentioning

confidence: 99%

“…The BVDV IHC testing was performed on formalin-fixed ear notches from 94 cattle in the study. 7 The IHC procedure was performed at the OADDL as described by using the primary anti-BVDV mAb 3.12 F1. 7 The BRSV and BCoV were assayed in 194 lung homogenates by using gel-based reverse transcription polymerase chain reaction (RT-PCR) assays.…”

Section: Agent Identificationmentioning

confidence: 99%

“…7 The IHC procedure was performed at the OADDL as described by using the primary anti-BVDV mAb 3.12 F1. 7 The BRSV and BCoV were assayed in 194 lung homogenates by using gel-based reverse transcription polymerase chain reaction (RT-PCR) assays. The RT-PCR product for the BRSV RT-PCR was a 481-bp region of the F gene.…”

Section: Agent Identificationmentioning

confidence: 99%

See 2 more Smart Citations

Lung Pathology and Infectious Agents in Fatal Feedlot Pneumonias and Relationship with Mortality, Disease Onset, and Treatments

Fulton¹,

Blood

Panciera³

et al. 2009

J VET Diagn Invest

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160

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Section: Agent Identificationmentioning

confidence: 99%

Section: Agent Identificationmentioning

confidence: 99%

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Lung Pathology and Infectious Agents in Fatal Feedlot Pneumonias and Relationship with Mortality, Disease Onset, and Treatments

Fulton¹,

Blood

Panciera³

et al. 2009

J VET Diagn Invest

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123

160

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“…3 Further, mortality rates among PI animals are 10-fold higher than those observed in non-PI animals. 5 Skin biopsies, serum, and buffy coat are the sample types most frequently collected for surveillance efforts, and commercial test kits have been developed based on testing of these sample types.…”

mentioning

confidence: 99%

Comparison of Detection of Bovine Virus Diarrhea Virus Antigen in Various Types of Tissue and Fluid Samples Collected from Persistently Infected Cattle

2011

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Abstract. Bovine viral diarrhea viruses are economically important pathogens of cattle. Most infections in susceptible animals are acquired from animals persistently infected with the virus. Surveillance programs rely on skin biopsies of persistently infected (PI) cattle to detect the infection. The purpose of this study was to compare antigen capture enzyme-linked immunosorbent assay (ACE) testing results using different types of samples from PI animals. The intent was to determine comparative detection rates in types of samples that are frequently submitted to diagnostic laboratories for evaluation of cases of unknown etiology or samples that could be easily collected for Bovine viral diarrhea virus (BVDV) screening. Eight types of samples were collected from 40 PI animals. The sample types were ear notches, serum, nasal swabs, conjunctival swabs, oral swabs, rectal swabs, vaginal/preputial swabs, and a tail skin fold biopsy. Each type of sample (n 5 8) for each animal (n 5 40) was evaluated with a commercial ACE kit. When using ACE, tail-skin fold and nasal swab samples were 100% sensitive compared with results using ear notches. Sensitivity using other samples was as follows: serum and vaginal/preputial swabs, 92%; conjunctival swabs, 64%; rectal swabs, 10%; oral swabs, 8%. Testing of tail skin fold biopsies, nasal swabs, and ear notch samples resulted in reliable results. In contrast, other sample types were unreliable for diagnosis of persistent infection in calves.

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“…In the USA, however, where beef cattle production is the highest in the world, the potential risk of transmission of both BVDV 1 and 2 is reserved for persistently infected (PI) dairy and beef cattle entering the feedlot. A recent study demonstrated that of 21,743 cattle entering the feedlot, 0.4% were PI (Fulton et al 2006). Among cattle herds (dairy and beef) seroprevalence varied between 19% and 89%, but the prevalence of PI animals in the cattle population ranged from 0.5% to 2% (Cowley et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Detection of the bovine viral diarrhoea virus (BVDV) in young beef cattle in eastern and south-eastern regions of Poland

Wernicki¹,

Urban‐Chmiel²,

Stęgierska³

et al. 2015

Polish Journal of Veterinary Sciences

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In view of the scarcity of information concerning viral diarrhoea virus (BVDV) infections in beef cattle in Poland, the aim of this study was to evaluate the presence of the BVDV in young beef cattle from selected herds in eastern and south-eastern regions of Poland. The material consisted of 78 sera obtained from beef cattle from 15 farms, aged 6-12 months. The anti-BVDV antibody level in the sera was estimated with an ELISA kit, and detection of the BVDV was carried out by standard PCR and one step Real-Time RT-PCR. The ELISA results showed a high degree (80%) of positivity in 5 of the 78 samples. In 7 samples the degree of positivity was in the very low range: ≤40%. Of the 78 cDNA samples, the presence of genetic material with a length of 288 bp was found by standard PCR in 3 sera. The genetic material of BVDV was also found in the sera of the same three calves by Real-Time HRM PCR. BVDV infection in young beef cattle in south-eastern Poland is not a significant problem. This was confirmed by the positive ELISA results for 6.4% of the animals and the positive PCR results for 3.9%. The percentage of positive beef herds was about 8.6%. However, due to the severe nature of the disease and rapid transmission of the virus, regular monitoring of BVDV should be carried out.

show abstract

Evaluation of diagnostic tests used for detection of bovine viral diarrhea virus and prevalence of subtypes 1a, 1b, and 2a in persistently infected cattle entering a feedlot

Cited by 85 publications

References 23 publications

Lung Pathology and Infectious Agents in Fatal Feedlot Pneumonias and Relationship with Mortality, Disease Onset, and Treatments

Lung Pathology and Infectious Agents in Fatal Feedlot Pneumonias and Relationship with Mortality, Disease Onset, and Treatments

Comparison of Detection of Bovine Virus Diarrhea Virus Antigen in Various Types of Tissue and Fluid Samples Collected from Persistently Infected Cattle

Detection of the bovine viral diarrhoea virus (BVDV) in young beef cattle in eastern and south-eastern regions of Poland

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