Evaluation of diagnostic tests for Johne's disease in young cattle

McDonald, W L; Ridge, S E; Hope, A. F.; Condron, R. J.

doi:10.1111/j.1751-0813.1999.tb11679.x

Cited by 94 publications

(90 citation statements)

References 17 publications

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“…A slight shedding of MAP in faeces of eight year old heifers was described (Antognoli et al (2007). McDonald et al (1999) described the shedding of MAP in the faeces of one to 18-monthold animals; nevertheless, they considered isolation from animals above 16 months as more common. According to Weber et al (2005), young animals become faecal positive when breeding in the herds with high prevalence of infection.…”

Section: Culture Examination Of Faecesmentioning

confidence: 99%

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

Hasoňová¹,

Trcka²,

Babák³

et al. 2009

Vet. Med. - Czech

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The objectives of this study were to investigate Mycobacterium avium subsp. paratuberculosis (MAP) distribution in various tissues, in faeces and in milk samples from 131 animals originating from one imported Jersey cattle herd from Denmark. MAP was detected by culture in 37.4% animals. Massive MAP growth was most often observed in the small intestines (48 animals). The lowest levels of MAP were found in spleen and mammary gland samples. MAP was detected in the faeces of 8.4% animals; however, milk samples were MAP negative by culture. The highest prevalence of MAP infection (42.9%) was in age Group B (1.6 to 3 years) and the lowest (6.1%) in group D (more than 8 years). Seven positive calves were detected in the youngest age group; one of them was less than one month of age, which could imply an intrauterine infection. MAP shedding in faeces by a five-month-old calf was also confirmed. The IS900 RFLP (restriction fragment length polymorphism) type B-C1 was identified in all animals.

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Section: Culture Examination Of Faecesmentioning

confidence: 99%

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

Hasoňová¹,

Trcka²,

Babák³

et al. 2009

Vet. Med. - Czech

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“…In studies which included multiple different challenge strains, only those animals identified as infected with bovine-specific MAP strains were included. Animals from farms with known MAP-positive animals or animals born from infected dams were excluded from the dataset with two exceptions: Taylor et al [18] and McDonald et al [28]. In the McDonald et al study, dams of calves enrolled in the study were testnegative at the time of study initiation but calves were not specifically from a MAP-free herd.…”

Section: Inclusion/exclusion Criteriamentioning

confidence: 99%

“…The sensitivity of culture was expected to be influenced by culture method and laboratory ; however, quantitative information on culture sensitivity by laboratory/specific method is lacking and changes in culture sensitivity were therefore not accounted for in the analysis. If animals were given two or more infectious doses of MAP in a short period of time and faecal cultures were reported in months postexposure, we assumed that values of time since infection were reported as time since first dose [4,5,[26][27][28][29][30].…”

Section: Standardization Of Experimental Data Across Studiesmentioning

confidence: 99%

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A meta-analysis of the effect of dose and age at exposure on shedding ofMycobacterium aviumsubspeciesparatuberculosis(MAP) in experimentally infected calves and cows

et al. 2011

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SUMMARYA meta-analysis was performed using all published and one unpublished long-term infection-challenge experiments to quantify the age- and dose-dependence of early and late shedding of Mycobacterium avium subsp. paratuberculosis (MAP) in cattle. There were 194 animals from 17 studies that fulfilled the inclusion criteria, of which 173 received a known dose of MAP and 21 were exposed naturally. Results from parametric time-to-event models indicated that challenging older calves or using multiple-exposure experimental systems resulted in a smaller proportion and shorter duration of early shedding as well as slower transition to late shedding from latent compartments. Calves exposed naturally showed variable infection progression rates, not dissimilar to other infection routes. The log-normal distribution was most appropriate for modelling infection-progression events. The infection pattern revealed by the modelling allowed better understanding of low-grade endemicity of MAP in cattle, and the parameter estimates are the basis for future transmission dynamics modelling.

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“…paratuberculosis (4) was successful in identifying animals in the early stages of infection. However, even uninfected animals may intermittently test positive, perhaps suggesting a lack of specificity inherent in the assay (16). Serological assays including agar gel immunodiffusion, enzyme-linked immunosorbent assay, and complement fixation tests have been developed to detect antibodies against M. avium subsp.…”

mentioning

confidence: 99%

Characterization of Novel Coding Sequences Specific to Mycobacterium avium subsp. paratuberculosis : Implications for Diagnosis of Johne's Disease

et al. 2004

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Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's Disease, an economically important intestinal ailment of ruminants. Due to the considerable genetic and serologic cross-reactivity with closely related and ubiquitous members of the M. avium complex, a species-specific method for the serological diagnosis of Johne's disease is unavailable. Computational and PCR-based analysis of the complete genome sequence of M. avium subsp. paratuberculosis led to the identification of 13 open reading frames with no identifiable homologs. One of these sequences is a putative insertion element present in six copies on the M. avium subsp. paratuberculosis genome. These novel M. avium subsp. paratuberculosis genes were cloned into Escherichia coli expression vectors, and nine were successfully expressed as recombinant fusion proteins. Five of these proteins were purified in sufficient amounts to allow immunoblot analyses of their reactivity with sera from naturally infected cattle as well as mice and rabbits exposed to M. avium subsp. paratuberculosis. Fusion proteins representing MAP0862, MAP3732c, and MAP2963c were recognized by nearly all of the sera tested, including those from cattle in the clinical stages of disease. Notably, further analysis of the protein encoded by MAP0862 showed that it reacted with sera from additional infected cattle but not with sera from uninfected control animals. The fusion product of MAP0860c did not react with any of the sera tested, while the remaining four proteins were variably recognized by sera from M. avium subsp. paratuberculosis-infected cattle. Collectively, the results of this study demonstrate the utility of genomic data to identify potential diagnostic sequences.

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Evaluation of diagnostic tests for Johne's disease in young cattle

Abstract: Detection of M paratuberculosis infection in young cattle continues to be difficult using current tests.

Cited by 94 publications

References 17 publications

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age

A meta-analysis of the effect of dose and age at exposure on shedding ofMycobacterium aviumsubspeciesparatuberculosis(MAP) in experimentally infected calves and cows

Characterization of Novel Coding Sequences Specific to Mycobacterium avium subsp. paratuberculosis : Implications for Diagnosis of Johne's Disease

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