Evaluation of diagnostic accuracy of developed rapid SARS-COV-2 IgG antibody test kit using novel diluent system

Maya, Vani; Kaviyil, Jyothi Embekkat; Ponnambath, Dinoop Koral; Nair, Renjith P.; Bhatt, Anugya; Pilankatta, Rajendra; Valliyot, Lathika; Dungdung, Ranjeet; Veettil, Ramdas Athikkal; Gopi, Manoj

doi:10.1007/s13337-021-00669-4

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pre-coated anti-SARS-CoV-2 antibody then captures the immune complex on the membrane, resulting in the formation of a colorful T line and a positive COVID-19 test result. Internal control (C line) in the test should display a colored line regardless of color development on the T line [16]. The test is invalid and the specimen must be retested using a new device if the C line does not develop.…”

Section: Procedures Of Nasopharyngeal Specimen Detection With Extract...mentioning

confidence: 99%

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Suresh¹,

Singha²,

Maheshwari³

et al. 2022

J App Biol Biotech

View full text Add to dashboard Cite

To stop the spread of COVID-19 in this outbreak, diagnostic testing is essential. Quick diagnostic tests must be employed in this pandemic, which is brought on by the severe acute respiratory syndrome (SARS)-CoV-2 virus, to successfully treat and manage COVID-19. There are several problems with the present RT-PCR system that the lateral flow assay (LFA), a kind of clinically sensitive diagnostic test, may be able to fix, especially in low-and middleincome nations. Gold nanoparticle-(AuNP-LFA) is a practical method for detecting COVID-19 in basic hospitals and laboratories, particularly in emergency situations where many samples must be quickly examined. Safe, accurate, and non-toxic diagnostic tests must be employed during the pandemic, to successfully treat and manage COVID-19. Recombinant SARS-CoV-2 nucleocapsid monoclonal antibody was employed to detect COVID-19 antigens in the presence of patients to establish a fast LFA for COVID-19. Synthesis of colloidal gold particles and antibody colloidal gold conjugates was evaluated by using UV/Vis spectroscopy. A capture line made of anti-SARS-CoV-2 antibody was coated on nitrocellulose membrane. To create the control line, goat anti-mouse IgG monoclonal antibody was coated. On a polystyrene backing board, the immunochromatographic strip was constructed in the ideal order. Using ELISA as the standard procedure, the strips' sensitivity and specificity were assessed. The results' stability and repeatability were evaluated over a 9-month period. Colloidal gold nanoparticle-based LFAs created in this study can be employed for quicker and more accurate detection of SARS-CoV-2.

show abstract

Section: Procedures Of Nasopharyngeal Specimen Detection With Extract...mentioning

confidence: 99%

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Suresh¹,

Singha²,

Maheshwari³

et al. 2022

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…99,130,145,147 These contrasting differences usually arise from the lack of a standard test evaluation protocol, and depend on the number of true positive/negative samples, age or pre-health conditions of volunteers, the severity of infection, test settings, etc. 161–165 Therefore, a standard test evaluation protocol is needed along with more extensive and well-planned evaluation studies to avoid false negative/positive results and improve diagnostic performance. Nonetheless, rapid antibody diagnostics will help in the epidemiology and assessment of both short-term and long-term immune system response and vaccine research, development, and effectiveness.…”

Section: Limitations Of Antibody Diagnostics and Future Outlookmentioning

confidence: 99%

Rapid antibody diagnostics for SARS-CoV-2 adaptive immune response

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, some publications have doubts about their diagnostic accuracy [ 8 , 11 – 13 ]. RDTs are not recommended for patient care but as a screening tool for suspects [ 14 , 15 ]. SARS-CoV-2 antibody detection can provide fast results to complement the time-consuming RT-PCR test [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Accuracy of Antibody-based Rapid Diagnostic Test in Detecting Coronavirus Disease 2019: Systematic Review

et al. 2021

View full text Add to dashboard Cite

IntroductionThe rapid transmission of Coronavirus disease 2019 (COVID-19) requires a fast, accurate, and affordable detection method. Despite doubts of its diagnostic accuracy, Rapid Diagnostic Test (RDT) is world-widely used in consideration for its practicality. This systematic review aims to determine the diagnostic accuracy of antibody-based RDT in detecting COVID-19.Material and methodsA literature search was carried out on five journal databases using the PRISMA-P 2015 method. We included all studies published up to February 2021. The risk of bias was evaluated using The Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Diagnostic Test Accuracy Studies. Data regarding peer-review status, study design, tests kit information, immunoglobulin class, target antigen, and the number of samples were extracted and tabulated. We estimated the pooled sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) with a 95% confidence interval.ResultsThirty-three studies met the eligibility criteria. The pooled data results showed that the combined detection method of IgM or IgG had the highest sensitivity and NPV, which were 73.41% (95% CI: 72.22-74.57) and 75.34% (95% CI: 74.51-76.16), respectively. The single IgG detection method had the highest specificity and PPV of 96.68% (95% CI: 96.25-97.07) and 95.97% (95% CI: 95.47-96.42%), respectively.ConclusionsAntibody-based RDT is not satisfactory as a primary diagnostic test but has utilities as a screening tool.

show abstract

Evaluation of diagnostic accuracy of developed rapid SARS-COV-2 IgG antibody test kit using novel diluent system

Cited by 3 publications

References 14 publications

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Rapid antibody diagnostics for SARS-CoV-2 adaptive immune response

Diagnostic Accuracy of Antibody-based Rapid Diagnostic Test in Detecting Coronavirus Disease 2019: Systematic Review

Contact Info

Product

Resources

About