Evaluation of Dextromethorphan HBr and Glyceryl Guaiacolate mixture in syrup preparation by Derivative Spectrophotometry Methods

Abstract

The derivative spectrophotometric methods are the goal of evaluating the simultaneous level of Dextromethorphan HBr and Glyceryl Guaiacolate mixture in Syrup. The determination of the mixture is used the derivative spectrophotometry method with methanol-water (50:50) for Dextromethorphan HBr and Glyceryl Guaiacolate. The derivative spectrophotometric method on 1st derivatization with ∆λ 2 nm have wavelength 263.6 nm for Dextromethorphan HBr and 2nd derivatization with ∆λ 2 nm at 285 nm for Glyceryl guaiacolate, The locally producted syrup samples met the level requirements mixture of Dextromethorphan and Glyceryl guaiacolate less than 90.0% and not over 110.0% of the amount stated on the label. The validation test showed that these two methods had met the validation parameter. The derivative spectrophotometry method was used to determine a mixture of Dextromethorphan HBr and Glyceryl guaiacolate and fulfilling the validation requirements and level requirements according to USP 30.