Evaluation of destaining methods in DNA cytofluorometry of the wright-giemsa stained normal and leukemic cells.

Shimazaki, Chihiro; Nakanishi, Satoshi; Haruyama, H; Isemura, T; Nakagawa, Masao; Ijichi, Hamao

doi:10.1267/ahc.16.13

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Dna Cytofluorometry Combined With Autoradiography1

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1990

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“…The smears were air-dried and fixed in 100% methanol and the nuclear reaction was performed with acriflavine-Shiff reagent after azocarmin G blockage of nonspecific staining [16,17). Feul gen-stained preparations were dipped in nuclear emulsion, stored at 4°C for 3 weeks and then developed.…”

Section: Dna Cytofluorometry Combined With Autoradiographymentioning

confidence: 99%

Giant Neutrophils Derived from Tetraploid Leukemic Clone in an Acute Myeloblastic Leukemia: Cytofluorometric Study

Fujita¹,

Shimazaki²,

Murakami³

et al. 1990

Acta Haematol

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Near-tetraploid chromosomes were observed in a patient with acute myeloblastic leukemia with maturation (M₂ in FAB classification). Large and morphologically bizarre leukemic cells and giant neutrophils in each maturation stage were observed in both peripheral blood and bone marrow. Cytogenetic studies revealed that the main stem line was 93; XXYY, +9, and DNA cytofluorometry showed that these large leukemic cells and giant neutrophils had 4C DNA content. These findings strongly suggested that these giant neutrophils were derived from leukemic clone with tetraploidy.

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Section: Dna Cytofluorometry Combined With Autoradiographymentioning

confidence: 99%