Evaluation of designer crops for biosafety—A scientist's perspective

Mehrotra, Shweta; Goyal, Vinod

doi:10.1016/j.gene.2012.12.029

Cited by 28 publications

(17 citation statements)

References 95 publications

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“…Stable integration and expression of the transferred gene are prerequisites for commercialization (Mehrotra and Goyal, 2013). In our research, the molecular characterization of I. variabilis-EPSPS * expression cassette was carefully performed with Southern blot, inverse PCR, Northern blot and Western blot.…”

Section: Discussionmentioning

confidence: 99%

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

et al. 2016

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Glyphosate is the most widely used herbicide for its low cost and high efficiency. However, it is rarely applied directly in rice field due to its toxicity to rice. Therefore, glyphosate-tolerant rice can greatly decrease the cost of rice production and provide a more effective weed management strategy. Although, several approaches to develop transgenic rice with glyphosate tolerance have been reported, the agronomic performances of these plants have not been well evaluated, and the feasibility of commercial production has not been confirmed yet. Here, a novel glyphosate-tolerant gene cloned from the bacterium Isoptericola variabilis was identified, codon optimized (designated as I. variabilis-EPSPS*), and transferred into Zhonghua11, a widely used japonica rice cultivar. After systematic analysis of the transgene integration via PCR, Southern blot and flanking sequence isolation, three transgenic lines with only one intact I. variabilis-EPSPS* expression cassette integrated into intergenic regions were identified. Seed test results showed that the glyphosate tolerance of the transgenic rice was about 240 times that of wild type on plant medium. The glyphosate tolerance of transgenic rice lines was further evaluated based on comprehensive agronomic performances in the field with T3 and T5generations in a 2-year assay, which showed that they were rarely affected by glyphosate even when the dosage was 8400 g ha−1. To our knowledge, this is the first demonstration of the development of glyphosate-tolerant rice lines based on a comprehensive analysis of agronomic performances in the field. Taken together, the results suggest that the selected glyphosate-tolerant rice lines are highly tolerant to glyphosate and have the possibility of commercial release. I. variabilis-EPSPS* also can be a promising candidate gene in other species for developing glyphosate-tolerant crops.

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Section: Discussionmentioning

confidence: 99%

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

et al. 2016

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“…To ensure biosafety, scientific expertise must be applied to the analysis of biotech crops, their progeny, and anticipated environmental interactions for a sound risk assessment, which ironically can be accomplished, in part, with omic studies. 57 …”

Section: Accomplishments and Challenges For Omicsmentioning

confidence: 99%

The Omics Revolution in Agricultural Research

Emon

2015

J. Agric. Food Chem.

111

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The Agrochemicals Division cosponsored the 13th International Union of Pure and Applied Chemistry International Congress of Pesticide Chemistry held as part of the 248th National Meeting and Exposition of the American Chemical Society in San Francisco, CA, USA, August 10–14, 2014. The topic of the Congress was Crop, Environment, and Public Health Protection; Technologies for a Changing World. Over 1000 delegates participated in the Congress with interactive scientific programming in nine major topic areas including the challenges and opportunities of agricultural biotechnology. Plenary speakers addressed global issues related to the Congress theme prior to the daily technical sessions. The plenary lecture addressing the challenges and opportunities that omic technologies provide agricultural research is presented here. The plenary lecture provided the diverse audience with information on a complex subject to stimulate research ideas and provide a glimpse of the impact of omics on agricultural research.

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“…It should take into account country's biosafety guidelines, food safety, and international agreements on genetically modified organisms and their derivatives since they aim to ensure human health, protect the environment, and preserve biodiversity. Biosafety guidelines aim to prevent, minimize, and eliminate any risks related to research, development, and use of genetically modified organisms, while food safety undertakes research on substantial equivalence based on compositional analysis, toxicity, and allergenicity (Mehrotra and Goyal 2013). Most of the research on environmental and biodiversity safety deals with unintended effect on non-target organisms and gene flow.…”

Section: Transgenic Crops "Risks"mentioning

confidence: 99%

Genetic Engineering and Transgenic Breeding

Ríos

2015

Plant Breeding in the Omics Era

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Evaluation of designer crops for biosafety—A scientist's perspective

Cited by 28 publications

References 95 publications

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

The Omics Revolution in Agricultural Research

Genetic Engineering and Transgenic Breeding

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