Evaluation of density gradient ultracentrifugation serum lipoprotein profiles in healthy dogs and dogs with exocrine pancreatic insufficiency

Minamoto, Tomomi; Parambeth, Joseph Cyrus; Walzem, Rosemary L.; Payne, H. Ross; Lidbury, Jonathan A.; Suchodolski, Jan S.; Steiner, Jörg M.

doi:10.1177/1040638718793677

Cited by 4 publications

(9 citation statements)

References 42 publications

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“…Lipoprotein profiling was carried out using a bismuth sodium ethylenediaminetetraacetic acid (NaBiEDTA) density gradient ultracentrifugation method as previously described . This method has been validated in dogs and used to evaluate lipoprotein profiles in healthy dogs and in dogs with idiopathic hyperlipidemia and exocrine pancreatic insufficiency . This method also has been used to evaluate lipoprotein profiles in cats with hepatic lipidosis .…”

Section: Methodsmentioning

confidence: 99%

“…The methodology used in our study previously was shown to identify 11 distinct lipoprotein fractions in dogs based solely on density characteristics . Because the functional characteristics and composition of most lipoprotein density subfractions in dogs currently are unknown, all density subfractions can only be nominally assigned to traditional functional classes such as low‐density lipoproteins (LDL) or high‐density lipoproteins (HDL).…”

Section: Methodsmentioning

confidence: 99%

“…Because the functional characteristics and composition of most lipoprotein density subfractions in dogs currently are unknown, all density subfractions can only be nominally assigned to traditional functional classes such as low‐density lipoproteins (LDL) or high‐density lipoproteins (HDL). Thus, density subfraction data are reported as previously described density ranges identified as R1 to R11 where: R1 ( d < 1.017 g/mL), R2 ( d = 1.019‐1.023 g/mL), R3 ( d = 1.023‐1.029 g/mL), R4 ( d = 1.029‐1.039 g/mL), R5 ( d = 1.039‐1.050 g/mL), R6 ( d = 1.050‐1.063 g/mL), R7 ( d = 1.063‐1.091 g/mL), R8 ( d = 1.091‐1.110 g/mL), R9 ( d = 1.110‐1.133 g/mL), R10 ( d = 1.133‐1.156 g/mL), and R11 ( d = 1.156‐1.179 g/mL) . Based on a previously published classification, and based solely on their density characteristics, these fractions could be classified as: triglyceride‐rich lipoproteins (TRL; chylomicrons and very low‐density lipoproteins [VLDL]; d < 1.017 g/mL), LDL 1 ( d = 1.019‐1.023 g/mL), LDL 2 ( d = 1.023‐1.029 g/mL), LDL 3 ( d = 1.029‐1.039 g/mL), LDL 4 ( d = 1.039‐1.050 g/mL), LDL 5 ( d = 1.050‐1.063 g/mL), HDL 2b ( d = 1.063‐1.091 g/mL), HDL 2a ( d = 1.091‐1.110 g/mL), HDL 3a ( d = 1.110‐1.133 g/mL), HDL 3b ( d = 1.133‐1.156 g/mL), and HDL 3c ( d = 1.156‐1.179 g/mL), respectively .…”

Section: Methodsmentioning

confidence: 99%

“…Based on a previously published classification, and based solely on their density characteristics, these fractions could be classified as: triglyceride‐rich lipoproteins (TRL; chylomicrons and very low‐density lipoproteins [VLDL]; d < 1.017 g/mL), LDL 1 ( d = 1.019‐1.023 g/mL), LDL 2 ( d = 1.023‐1.029 g/mL), LDL 3 ( d = 1.029‐1.039 g/mL), LDL 4 ( d = 1.039‐1.050 g/mL), LDL 5 ( d = 1.050‐1.063 g/mL), HDL 2b ( d = 1.063‐1.091 g/mL), HDL 2a ( d = 1.091‐1.110 g/mL), HDL 3a ( d = 1.110‐1.133 g/mL), HDL 3b ( d = 1.133‐1.156 g/mL), and HDL 3c ( d = 1.156‐1.179 g/mL), respectively . The above‐mentioned previously reported classification scheme for lipoprotein subfractions was used in our study …”

Section: Methodsmentioning

confidence: 99%

“…19 This method has been T A B L E 1 Characteristics of dogs with pancreatitis and healthy control dogs included into the study validated in dogs 20 and used to evaluate lipoprotein profiles in healthy dogs 21 and in dogs with idiopathic hyperlipidemia 21 and exocrine pancreatic insufficiency. 20 This method also has been used to evaluate lipoprotein profiles in cats with hepatic lipidosis. 22 The sodium salt of BiEDTA has been described as a novel solute forming a self-generating density gradient during ultracentrifugation of serum samples for the separation of lipoproteins.…”

Section: Lipoprotein Profile Analysismentioning

confidence: 99%

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Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Xenoulis

Cammarata

Walzem

et al. 2020

Veterinary Internal Medicne

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Background Previous studies have reported an association between hyperlipidemia and pancreatitis in dogs, but details of this association remain poorly defined. Hypothesis/Objectives To compare serum triglyceride and cholesterol concentrations and lipoprotein profiles between dogs with naturally occurring pancreatitis and healthy dogs. Animals Seventeen dogs with a clinical diagnosis of pancreatitis (Group 1) and 53 healthy control dogs (Group 2). Methods Prospective case‐control study. Results In Group 1, 3/17 dogs (18%) had hypertriglyceridemia whereas in Group 2, 4/53 dogs (7.5%) had hypertriglyceridemia (odds ratio [OR], 2.63; 95% confidence interval [CI], 0.52‐13.14; P = .35). A significant difference was found in serum triglyceride concentrations between Group 1 (median, 67 mg/dL) and Group 2 (median, 54 mg/dL; P = .002). In Group 1, 4/17 dogs (24%) had hypercholesterolemia, whereas 1/53 (1.9%) dogs in Group 2 had hypercholesterolemia (OR, 16; 95% CI, 1.64‐155.5; P = .01). No significant difference was found in serum cholesterol concentrations between Group 1 (median, 209 mg/dL) and Group 2 (median, 227 mg/dL; P = .56). Lipoprotein profiles were significantly different between Group 1 and Group 2 dogs (Eigenvalues, 0.6719; R2 = 1.0; P = .001). Conclusions and Clinical Importance Most dogs with pancreatitis (>70%) had serum triglyceride and cholesterol concentrations within reference intervals. In the small percentage of dogs that had hypertriglyceridemia, hypercholesterolemia, or both, increases were mild. Important differences were identified in lipoprotein profiles between dogs with pancreatitis and healthy control dogs. Dogs with pancreatitis had higher low‐density lipoprotein fractions and lower triglyceride‐rich lipoprotein and high‐density lipoprotein fractions than healthy dogs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Lipoprotein Profile Analysismentioning

confidence: 99%

See 3 more Smart Citations

Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Xenoulis

Cammarata

Walzem

et al. 2020

Veterinary Internal Medicne

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Clustering analysis of lipoprotein profiles to identify subtypes of hypertriglyceridemia in Miniature Schnauzers

Tate,

Yamkate,

Xenoulis

et al. 2024

Veterinary Internal Medicne

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BackgroundHypertriglyceridemia (HTG) is prevalent in Miniature Schnauzers, predisposing them to life‐threatening diseases. Varied responses to management strategies suggest the possibility of multiple subtypes.Hypothesis/ObjectiveTo identify and characterize HTG subtypes in Miniature Schnauzers through cluster analysis of lipoprotein profiles. We hypothesize that multiple phenotypes of primary HTG exist in this breed.AnimalsTwenty Miniature Schnauzers with normal serum triglyceride concentration (NTG), 25 with primary HTG, and 5 with secondary HTG.MethodsCross‐sectional study using archived samples. Lipoprotein profiles, generated using continuous lipoprotein density profiling, were clustered with hierarchical cluster analysis. Clinical data (age, sex, body condition score, and dietary fat content) was compared between clusters.ResultsSix clusters were identified. Dogs with primary HTG were dispersed among 4 clusters. One cluster showed the highest intensities for triglyceride‐rich lipoprotein (TRL) and low‐density lipoprotein (LDL) fractions and also included 4 dogs with secondary HTG. Two clusters had moderately high TRL fraction intensities and low‐to‐intermediate LDL intensities. The fourth cluster had high LDL but variable TRL fraction intensities with equal numbers of NTG and mild HTG dogs. The final 2 clusters comprised only NTG dogs with low TRL intensities and low‐to‐intermediate LDL intensities. The clusters did not appear to be driven by differences in the clinical data.Conclusions and Clinical ImportanceThe results of this study support a spectrum of lipoprotein phenotypes within Miniature Schnauzers that cannot be predicted by triglyceride concentration alone. Lipoprotein profiling might be useful to determine if subtypes have different origins, clinical consequences, and response to treatment.

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Blood taurine concentrations in dogs with exocrine pancreatic insufficiency

Tayler¹,

Seo²,

Connolly³

et al. 2020

ajvr

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OBJECTIVE To determine whether blood taurine concentrations in dogs with exocrine pancreatic insufficiency (EPI) were lower than the reference interval (200 to 350 nmol/mL) or the cutoff used to indicate taurine deficiency (< 150 nmol/mL). ANIMALS 18 dogs with clinical or presumptive subclinical EPI with residual blood samples available for taurine concentration analysis. PROCEDURES Dogs were classified as having clinical EPI if they had a serum trypsin-like immunoreactivity concentration of < 2.0 μg/L and presumptive subclinical EPI if they had a concentration of 2.0 to 5.0 μg/L. Archived, frozen blood samples stored in EDTA were submitted for measurement of taurine concentration with an automated high-performance liquid chromatography amino acid analyzer. Medical record data were examined for associations with blood taurine concentration. RESULTS None of the 18 dogs had a blood taurine concentration < 150 nmol/mL. Two dogs had a concentration < 200 nmol/mL. No clinical signs, physical examination findings, or serum biochemical abnormalities were associated with blood taurine concentration. Eleven of the 17 dogs for which diet histories were available were not receiving a diet that met recommendations of the World Small Animal Veterinary Association Global Nutrition Committee. CONCLUSIONS AND CLINICAL RELEVANCE A low blood taurine concentration was noted in a small subset of dogs with EPI. Additional research is needed to determine whether EPI was the primary cause of this low concentration. Findings suggested the importance of obtaining complete diet histories and ensuring dietary requirements are sufficiently met in dogs with EPI. (Am J Vet Res 2020;81:958–963)

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Evaluation of density gradient ultracentrifugation serum lipoprotein profiles in healthy dogs and dogs with exocrine pancreatic insufficiency

Cited by 4 publications

References 42 publications

Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Clustering analysis of lipoprotein profiles to identify subtypes of hypertriglyceridemia in Miniature Schnauzers

Blood taurine concentrations in dogs with exocrine pancreatic insufficiency

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