Evaluation of dengue nonstructural protein 1 antigen strip for the rapid diagnosis of patients with dengue infection

Dengue virus non-structural protein NS1: Structure, intracellular trafficking and role as a diagnostic marker

“…By day 5, detection dropped to 50%, then decreased throughout the remainder of the disease period. These results are consistent with the findings of others who have used commercial NS1 capture ELISAs to detect NS1 (Bessoff et al, 2008;Blacksell et al, 2008; Dengue Diagnosis Chaiyaratana et al, 2009;Dussart et al, 2006;Dussart et al, 2008;Kumarasamy et al, 2007a;Kumarasamy et al, 2007b;Zainah et al, 2009). As with the current analysis these studies found that NS1 capture ELISA's had high sensitivity and specificity early during the acute period with detection decreasing during infection.…”

“…Since then, a number of companies have placed capture NS1 ELISA's on the market which have become the new standard for dengue diagnosis (Alcon et al, 2002;Bessoff et al, 2008;Blacksell et al, 2008;Chaiyaratana et al, 2009;Dussart et al, 2006;Dussart et al, 2008;Hsieh and Chen, 2009;Kumarasamy et al, 2007a;Kumarasamy et al, 2007b;Lapphra et al, 2008;Lemes et al, 2005;Libraty et al, 2002;Ludert et al, 2008;McBride, 2009;Phuong et al, 2009;Qiu et al, 2009;Shu et al, 2009;Simmons et al, 2007;Xu et al, 2006;Young et al, 2000;Zainah et al, 2009). What makes NS1 such an ideal target as a diagnostic tool is the fact that it is secreted at high levels into the blood of an infected individual and can be detected from the onset of symptoms, through to 9 days post disease onset.…”

Evaluation of the Dengue NS1 Ag Strip®for Detection of Dengue Virus Antigen inAedes aegypti(Diptera: Culicidae)

“…However, to date, none has been validated for DENV detection in its mosquito vectors. Recently, the Dengue NS1 Ag Strip Ò (BioRad Laboratories), a rapid immunochromatographic assay, has been developed to detect DENV NS1 antigen from acute patient serum samples (Dussart et al 2008, Chaiyaratana et al 2009, Pok et al 2010. NS1 is a structural protein that is produced in both membrane-associated and secreted forms.…”

Rapid detection of Bacillus anthracis by γ phage amplification and lateral flow immunochromatography

“…Since then, LFI devices have been utilized for detection of a large number of human bacterial pathogens, albeit with varying levels of success and/or sensitivity (Aldus et al, 2003;Clavijo et al, 2003;Kato et al, 2004;Zuiderwijk et al, 2003). LFI has also been successfully applied to detection of a number of human (Al-Yousif et al, 2002;Chaiyaratana et al, 2009;Kikuta et al, 2008;Li et al, 2009), plant (Kusano et al, 2007) and animal viral pathogens (Ferris et al, 2009;Joon Tam et al, 2004;Lyoo et al, 2005;Sithigorngul et al, 2007). Because of its wide application in a large number of bacterial and viral detection methods, LFI is an ideal platform for phage-based bacterial ID.…”