Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction

Chai, Yunsheng; Xu, Jian; Zhang, Y; Zhang, Junming; Hu, Zhiqian; Zhou, Haiyang

doi:10.1007/s10029-019-01954-4

Cited by 5 publications

(11 citation statements)

References 31 publications

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“…Although other studies have reported a significant reduction of DNA content (up to 98%) after NaOH‐based decellularization, 21‐23 the DNA content was decreased only by 41% compared to native vessels in this study (Figure 2). DNase treatment, as well as incubation with FCS, reduced the DNA content by 89% or 92%, respectively, compared to native vessels.…”

Section: Discussioncontrasting

confidence: 82%

“…For instance, Daugs et al reported that perfusion of bovine carotid arteries at high, physiological rates resulted in a better removal of cellular material compared to low-flow rates employing 1% of sodium deoxycholate solution as decellularization agent. 33 Although other studies have reported a significant reduction of DNA content (up to 98%) after NaOH-based decellularization, [21][22][23] the DNA content was decreased only by 41% compared to native vessels in this study (Figure 2). DNase treatment, as well as incubation with FCS, reduced the DNA content by 89% or 92%, respectively, compared to native vessels.…”

Section: Discussioncontrasting

confidence: 72%

“…Extensive washing removes toxicity, but does not completely eliminate detergents from the matrix 20 . Several studies have been published employing sodium hydroxide (NaOH) solution for the decellularization of diverse tissue types under varying concentrations 21‐24 . These studies presented efficient decellularization and structural preservation.…”

Section: Introductionmentioning

confidence: 99%

“…20 Several studies have been published employing sodium hydroxide (NaOH) solution for the decellularization of diverse tissue types under varying concentrations. [21][22][23][24] These studies presented efficient decellularization and structural preservation. Decellularization with NaOH solution is advantageous as it is a rather quick method leading to efficient decellularization within hours, may deactivate present pathogenic material, such as prions 25 and viruses, and is easy to neutralize and wash out leaving no harmful residues from the decellularization treatment itself behind.…”

mentioning

confidence: 99%

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Re‐endothelialization of non‐detergent decellularized porcine vessels

et al. 2020

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The optimal vascular graft should exhibit sufficient mechanical strength, low immunogenicity, high biocompatibility, and resistance to calcification, thrombosis, stenosis, and infection. 1 From a surgical perspective, it should be easy to handle, have reasonable manufacturing costs, and be readily available. Large diameter vascular grafts (>5 mm luminal diameter) have been successfully developed, but smaller vascular grafts (<5 mm luminal diameter) are difficult to generate because of blood clotting, scarring, or occlusion after implantation. 1 The materials used to construct vascular grafts fall into two categories: synthetic or biologic. 2 Synthetic scaffolds are either made from nondegradable polymers such as polytetrafluoroethylene and Dacron, which are widely used in the clinic, or biodegradable polymers such as poly(lactic-acid) and poly(glycolic-acid). 1,2 Synthetic scaffolds are advantageous with respect to the manufacturing process providing reproducible properties such as mechanical strength, size, and configuration. 1 However, current synthetic models are unable to actively respond to cellular signals and often lack the ability to host cell attachment or growth. 3,4 Biologic scaffolds are

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Section: Discussioncontrasting

confidence: 82%

Section: Discussioncontrasting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Re‐endothelialization of non‐detergent decellularized porcine vessels

et al. 2020

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“…Besides, nature SIS is composed of collagen, glycoproteins, glycosaminoglycans (GAGs), and proteoglycans should capture greater cellular signalling than other scaffold materials [11]. In the recent decade, the SIS has been used as a transplanted structural material in several clinical applications, such as for chronic wounds and burns [12], abdominal walls [13,14], cardiovascular tissue [15], and as a cell scaffold for intervertebral disc reconstructions [16]. After transplantation, the SIS was replaced by collagens [17].…”

Section: Introductionmentioning

confidence: 99%

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

et al. 2023

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Peripheral nerve regeneration (PNR) following trauma requires the reconstruction of the extracellular matrix (ECM) and the proper stimulation of growth factors. Decellularized small intestine submucosa (SIS) has been extensively used as an ECM scaffold for tissue repair, but its potential to enhance the effects of exogenous growth factors on PNR is not well understood. In this study, we evaluated the effects of SIS implantation combined with glial cell-derived growth factor (GDNF) treatment on PNR in a rat neurorrhaphy model. We found that both SIS and regenerating nerve tissue expressed syndecan-3 (SDC3), one of major heparan sulphate proteoglycans (HSPG) in nerve tissue, and that SDC3 interacted with GDNF in the regenerating nerve tissue. Importantly, the SIS-GDNF combined treatment enhanced the recovery of neuromuscular function and β3-tubulin-positive axonal outgrowth, indicating an increase in the number of functioning motor axons connecting to the muscle after neurorrhaphy. Our findings suggest that the SIS membrane offers a new microenvironment for neural tissue and promotes neural regeneration based on SDC3-GDNF signaling, providing a potential therapeutic approach for PNR.

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Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal

Tao

Mao

et al. 2021

Bioactive Materials

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Sterilization is the process of killing all microorganisms, while disinfection is the process of killing or removing all kinds of pathogenic microorganisms except bacterial spores. Biomaterials involved in cell experiments, animal experiments, and clinical applications need to be in the aseptic state, but their physical and chemical properties as well as biological activities can be affected by sterilization or disinfection. Decellularized matrix (dECM) is the low immunogenicity material obtained by removing cells from tissues, which retains many inherent components in tissues such as proteins and proteoglycans. But there are few studies concerning the effects of sterilization or disinfection on dECM, and the systematic introduction of sterilization or disinfection for dECM is even less. Therefore, this review systematically introduces and analyzes the mechanism, advantages, disadvantages, and applications of various sterilization and disinfection methods, discusses the factors influencing the selection of sterilization and disinfection methods, summarizes the sterilization and disinfection methods for various common dECM, and finally proposes a graphical route for selecting an appropriate sterilization or disinfection method for dECM and a technical route for validating the selected method, so as to provide the reference and basis for choosing more appropriate sterilization or disinfection methods of various dECM.

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Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction

Cited by 5 publications

References 31 publications

Re‐endothelialization of non‐detergent decellularized porcine vessels

Re‐endothelialization of non‐detergent decellularized porcine vessels

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*

Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal

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Evaluation of decellularization protocols for production of porcine small intestine submucosa for use in abdominal wall reconstruction

Cited by 5 publications

References 31 publications

Re‐endothelialization of non‐detergent decellularized porcine vessels

Re‐endothelialization of non‐detergent decellularized porcine vessels

Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study *

Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal

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Small intestine submucosa as a growth factor attractor promotes peripheral nerve regeneration by enhancing syndecan-3/glial cell line-derived neurotrophic factor (GDNF) signalling: in vivo study ^*